Transcript: Mouse NM_001177773.1

Mus musculus F-box and WD-40 domain protein 7 (Fbxw7), transcript variant 2, mRNA.

Source:
NCBI, updated 2017-06-25
Taxon:
Mus musculus (mouse)
Gene:
Fbxw7 (50754)
Length:
4183
CDS:
375..2507

Additional Resources:

NCBI RefSeq record:
NM_001177773.1
NBCI Gene record:
Fbxw7 (50754)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Mouse NM_001177773.1, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Mouse Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000365897 TTCAACAAGAACTTCGTAATT pLKO_005 2277 CDS 100% 13.200 18.480 N Fbxw7 n/a
2 TRCN0000373991 ACTTCTACTGTACGGTGTATG pLKO_005 1764 CDS 100% 10.800 15.120 N Fbxw7 n/a
3 TRCN0000365828 ATCCGAAACCTCGTCACATTG pLKO_005 2352 CDS 100% 10.800 15.120 N Fbxw7 n/a
4 TRCN0000012832 CGCATAGTTAGTGGTTCTGAT pLKO.1 1560 CDS 100% 4.950 6.930 N Fbxw7 n/a
5 TRCN0000012831 CGGAGGATTACATCTGTCCAA pLKO.1 1050 CDS 100% 2.640 3.696 N Fbxw7 n/a
6 TRCN0000006555 CCTAAAGAGTTGGCACTCTAT pLKO.1 1236 CDS 100% 0.495 0.693 N FBXW7 n/a
7 TRCN0000365827 CCCATGGAAGAGTGCGTATAT pLKO_005 1430 CDS 100% 13.200 9.240 N Fbxw7 n/a
8 TRCN0000365826 CTCTCAGGGTTTGGGATATTG pLKO_005 1828 CDS 100% 13.200 9.240 N Fbxw7 n/a
9 TRCN0000373989 TGTTAGTGGAGCTTATGATTT pLKO_005 1925 CDS 100% 13.200 9.240 N Fbxw7 n/a
10 TRCN0000373990 CAGCACAGAATTGATACAAAC pLKO_005 1455 CDS 100% 10.800 7.560 N Fbxw7 n/a
11 TRCN0000012828 CCAGTCATTAACGAGTGGAAT pLKO.1 2123 CDS 100% 4.950 3.465 N Fbxw7 n/a
12 TRCN0000012830 CCAGTGTTTACACGTCCTGAT pLKO.1 1856 CDS 100% 4.050 2.835 N Fbxw7 n/a
13 TRCN0000235423 TGATGGCAGGAGGGTTGTTAG pLKO_005 1910 CDS 100% 10.800 6.480 N FBXW7 n/a
14 TRCN0000087583 AGGAGGAAGAGGAAGAGGAAA pLKO.1 709 CDS 100% 4.950 2.475 Y Adam32 n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript NM_001177773.1, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_03574 pDONR223 100% 90.7% 96% None (many diffs) n/a
2 ccsbBroad304_03574 pLX_304 0% 90.7% 96% V5 (many diffs) n/a
3 TRCN0000477223 TGTCTGGTCTCAACTGTCAGACCT pLX_317 21.4% 90.7% 96% V5 (many diffs) n/a
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