Construct: ORF TRCN0000477279
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF011126.1_s317c1
- Derived from:
- ccsbBroadEn_06510
- DNA Barcode:
- GGCACAGACCCCCTATCAGTTGAG
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- KRT34 (3885)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000477279
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 100653049 | LOC100653049 | keratin, type I cuticular Ha4 | XM_003403882.5 | 100% | 100% | |
| 2 | human | 3885 | KRT34 | keratin 34 | NM_021013.3 | 99.8% | 99.7% | 441C>G;839T>C |
| 3 | human | 100653049 | LOC100653049 | keratin, type I cuticular Ha4 | XM_011547005.2 | 93.2% | 91.5% | (many diffs) |
| 4 | human | 3885 | KRT34 | keratin 34 | XM_011524793.2 | 93% | 91.2% | (many diffs) |
| 5 | human | 3884 | KRT33B | keratin 33B | NM_002279.5 | 81% | 79.5% | (many diffs) |
| 6 | human | 3883 | KRT33A | keratin 33A | NM_004138.4 | 79.8% | 79.8% | (many diffs) |
| 7 | human | 3881 | KRT31 | keratin 31 | NM_002277.3 | 77.6% | 76.4% | (many diffs) |
| 8 | human | 3883 | KRT33A | keratin 33A | XM_011524786.3 | 46.1% | 43.3% | (many diffs) |
| 9 | mouse | 16672 | Krt34 | keratin 34 | NM_027563.4 | 79% | 79.1% | (many diffs) |
| 10 | mouse | 16671 | Krt33b | keratin 33B | NM_013570.1 | 75.8% | 74.6% | (many diffs) |
| 11 | mouse | 71888 | Krt33a | keratin 33A | NM_027983.3 | 75.5% | 75.1% | (many diffs) |
| 12 | mouse | 16660 | Krt31 | keratin 31 | NM_010659.2 | 72.1% | 73.1% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1374
- ORF length:
- 1308
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgct gtatgccaag cccccaccca caattaatgg tataaaagga ctgcagagga 121 aggagagact caaacctgcc cacatccacc tccagcagct tacctgcttt tccattacct 181 gttccagcac catgtcttac agttgttgcc tgcccagcct gggctgccgc accagctgct 241 cctcccggcc ctgcgtgccc cccagctgcc acggctacac cctgcctggg gcctgcaaca 301 tccccgccaa tgtgagcaac tgcaactggt tctgtgaggg ctccttcaat ggcagcgaga 361 aggagactat gcagttcctg aacgaccgcc tggccagcta cctggagaag gtgcgtcagc 421 tggagcggga caacgcggag ctggagaaac tcatccagga gcggtcccag cagcaggagc 481 ccttgctgtg ccccagctac cagtcgtact tcaagaccat tgaggagctc cagcagaaga 541 ttctgtgtgc caaggctgag aatgccaggc tggtggtgaa cattgacaat gccaagctgg 601 cctctgacga cttcagaagc aagtaccaga cggagcagtc cctgaggctg ttggtggagt 661 cggacatcaa cagcatacgc aggatcctgg atgagctgac cctctgcaag tctgacctgg 721 agtcccaggt ggagtccctg agggaggagc tgatctgctt gaagaagaac catgaggagg 781 aggttaacac cctgcgctcc cagcttggag accgcctcaa cgtggaggtg gacactgccc 841 ccactgtgga cctgaaccag gtcctgaacg agaccaggag tcagtatgag gctctggtgg 901 aaactaaccg cagggaagtg gagcaatggt tcgccacgca gaccgaggag ctgaacaagc 961 aggtggtatc cagctcagag cagctgcagt cctgccaggc ggagatcatc gagctgagac 1021 gcacagtcaa cgccctggag atcgagctgc aggcccagca caaccTGCGA GACTCTCTGG 1081 AAAACACGCT GACGGAGAGC GAGGCCCACT ACAGCTCCCA GCTGTCCCAG GTGCAGAGCC 1141 TGATCACCAA CGTGGAGTCT CAGCTGGCAG AGATCCGCTG TGACCTGGAG CGGCAGAACC 1201 AGGAGTACCA GGTGCTGCTG GACGTGCGTG CCCGGCTGGA GTGTGAGATC AACACGTACC 1261 GGAGCCTCCT GGAGAGTGAG GACTGCAAGC TCCCCTGCAA CCCATGCGCC ACCACCAATG 1321 CTAGTGGCAA CTCCTGTGGA CCCTGTGGCA CCTCTCAAAA GGGTTGCTGT AATTGCCCAA 1381 CTTTCTTGTA CAAAGTGGTT GATATCGGTA AGCCTATCCC TAACCCTCTC CTCGGTCTCG 1441 ATTCTACGTA GTAATGAACT AGTCCGTAAC TTGAAAGTAT TTCGATTTCT TGGCTTTATA 1501 TATCTTGTGG AAAGGACGAG GCACAGACCC CCTATCAGTT GAGACGCGTT AAGTCgacaa 1561 tcaacctctg gattacaaaa tttgtgaaag att