Construct: ORF TRCN0000477588
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF018326.1_s317c1
- Derived from:
- ccsbBroadEn_00411
- DNA Barcode:
- ATCTATTATTTATGACGGCGACCT
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- CYP3A4 (1576)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000477588
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 1576 | CYP3A4 | cytochrome P450 family 3 su... | NM_017460.6 | 100% | 100% | |
| 2 | human | 1576 | CYP3A4 | cytochrome P450 family 3 su... | NM_001202855.3 | 99.8% | 99.6% | 670_671insCAG |
| 3 | human | 1551 | CYP3A7 | cytochrome P450 family 3 su... | NM_000765.5 | 94.4% | 88.4% | (many diffs) |
| 4 | human | 1577 | CYP3A5 | cytochrome P450 family 3 su... | NM_000777.5 | 89.2% | 84% | (many diffs) |
| 5 | human | 100861540 | CYP3A7-CYP3A51P | CYP3A7-CYP3A51P readthrough | NM_001256497.3 | 88.6% | 82.4% | (many diffs) |
| 6 | human | 1577 | CYP3A5 | cytochrome P450 family 3 su... | NM_001291830.2 | 86% | 80.7% | (many diffs) |
| 7 | human | 1577 | CYP3A5 | cytochrome P450 family 3 su... | NM_001291829.2 | 69.8% | 66.2% | (many diffs) |
| 8 | human | 64816 | CYP3A43 | cytochrome P450 family 3 su... | XM_017012544.1 | 60.4% | 54.3% | (many diffs) |
| 9 | human | 64816 | CYP3A43 | cytochrome P450 family 3 su... | XM_017012545.1 | 60.4% | 54.3% | (many diffs) |
| 10 | human | 1577 | CYP3A5 | cytochrome P450 family 3 su... | NR_033807.3 | 40.7% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1575
- ORF length:
- 1509
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatggc tctcatccca gacttggcca tggaaacctg gcttctcctg gctgtcagcc 121 tggtgctcct ctatctatat ggaacccatt cacatggact ttttaagaag cttggaattc 181 cagggcccac acctctgcct tttttgggaa atattttgtc ctaccataag ggcttttgta 241 tgtttgacat ggaatgtcat aaaaagtatg gaaaagtgtg gggcttttat gatggtcaac 301 agcctgtgct ggctatcaca gatcctgaca tgatcaaaac agtgctagtg aaagaatgtt 361 attctgtctt cacaaaccgg aggccttttg gtccagtggg atttatgaaa agtgccatct 421 ctatagctga ggatgaagaa tggaagagat tacgatcatt gctgtctcca accttcacca 481 gtggaaaact caaggagatg gtccctatca ttgcccagta tggagatgtg ttggtgagaa 541 atctgaggcg ggaagcagag acaggcaagc ctgtcacctt gaaagacgtc tttggggcct 601 acagcatgga tgtgatcact agcacatcat ttggagtgaa catcgactct ctcaacaatc 661 cacaagaccc ctttgtggaa aacaccaaga agcttttaag atttgatttt ttggatccat 721 tctttctctc aataacagtc tttccattcc tcatcccaat tcttgaagta ttaaatatct 781 gtgtgtttcc aagagaagtt acaaattttt taagaaaatc tgtaaaaagg atgaaagaaa 841 gtcgcctcga agatacacaa aagcaccgag tggatttcct tcagctgatg attgactctc 901 agaattcaaa agaaactgag tcccacaaag ctctgtccga tctggagctc gtggcccaat 961 caattatctt tatttttgct ggctatgaaa ccacgagcag tgttctctcc ttcattatgt 1021 atgaactggc cactcaccct gatGTCCAGC AGAAACTGCA GGAGGAAATT GATGCAGTTT 1081 TACCCAATAA GGCACCACCC ACCTATGATA CTGTGCTACA GATGGAGTAT CTTGACATGG 1141 TGGTGAATGA AACGCTCAGA TTATTCCCAA TTGCTATGAG ACTTGAGAGG GTCTGCAAAA 1201 AAGATGTTGA GATCAATGGG ATGTTCATTC CCAAAGGGGT GGTGGTGATG ATTCCAAGCT 1261 ATGCTCTTCA CCGTGACCCA AAGTACTGGA CAGAGCCTGA GAAGTTCCTC CCTGAAAGAT 1321 TCAGCAAGAA GAACAAGGAC AACATAGATC CTTACATATA CACACCCTTT GGAAGTGGAC 1381 CCAGAAACTG CATTGGCATG AGGTTTGCTC TCATGAACAT GAAACTTGCT CTAATCAGAG 1441 TCCTTCAGAA CTTCTCCTTC AAACCTTGTA AAGAAACACA GATCCCCCTG AAATTAAGCT 1501 TAGGAGGACT TCTTCAACCA GAAAAACCCG TTGTTCTAAA GGTTGAGTCA AGGGATGGCA 1561 CCGTAAGTGG AGCCTGCCCA ACTTTCTTGT ACAAAGTGGT TGATATCGGT AAGCCTATCC 1621 CTAACCCTCT CCTCGGTCTC GATTCTACGT AGTAATGAAC TAGTCCGTAA CTTGAAAGTA 1681 TTTCGATTTC TTGGCTTTAT ATATCTTGTG GAAAGGACGA ATCTATTATT TATGACGGCG 1741 ACCTACGCGT TAAGTCgaca atcaacctct ggattacaaa atttgtgaaa gatt