Construct: ORF TRCN0000477592

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF007847.1_s317c1
Derived from:
ccsbBroadEn_12238
DNA Barcode:
CCTTTCAAACCCGATGTTAGGCCA
Epitope Tag:
V5
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
SMPD4 (55627)

Vector Information:

Vector Backbone:
pLX_317
Pol II Cassette 1:
SV40-PuroR
Pol II Cassette 2:
EF1a-TRCN0000477592
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
V5

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 55627 SMPD4 sphingomyelin phosphodieste... XM_011511445.2 99.9% 100% 459G>A
2 human 55627 SMPD4 sphingomyelin phosphodieste... NM_001171083.2 87.5% 85.3% (many diffs)
3 human 55627 SMPD4 sphingomyelin phosphodieste... NM_017951.4 84.8% 84.8% 1_393del;852G>A
4 human 55627 SMPD4 sphingomyelin phosphodieste... NM_017751.4 81.4% 81.5% 1_393del;852G>A;981_982ins87
5 human 55627 SMPD4 sphingomyelin phosphodieste... XM_011511443.2 77.8% 77.8% 1_627del;1086G>A
6 human 55627 SMPD4 sphingomyelin phosphodieste... XM_017004448.2 76% 71.3% 0_1ins466;210_291del
7 human 55627 SMPD4 sphingomyelin phosphodieste... XM_024452978.1 76% 71.3% 0_1ins466;210_291del
8 human 55627 SMPD4 sphingomyelin phosphodieste... XM_011511444.3 74.7% 74.7% 1_627del;1086G>A;1215_1216ins87
9 human 55627 SMPD4 sphingomyelin phosphodieste... NR_033231.3 61.1% 1_251del;710G>A;2457_3606del
10 human 55627 SMPD4 sphingomyelin phosphodieste... XM_017004449.2 58.2% 58.2% 0_1ins921
11 human 55627 SMPD4 sphingomyelin phosphodieste... NR_033232.3 57.1% (many diffs)
12 human 150776 LOC150776 sphingomyelin phosphodieste... NR_026922.1 48.4% (many diffs)
13 human 55627 SMPD4 sphingomyelin phosphodieste... XR_002959315.1 27.2% (many diffs)
14 mouse 77626 Smpd4 sphingomyelin phosphodieste... NM_029945.3 75.4% 79.3% (many diffs)
15 mouse 77626 Smpd4 sphingomyelin phosphodieste... XM_006522722.2 75.4% 79.3% (many diffs)
16 mouse 77626 Smpd4 sphingomyelin phosphodieste... XM_006522720.3 72.8% 76.5% (many diffs)
17 mouse 77626 Smpd4 sphingomyelin phosphodieste... NM_001164610.1 72.1% 76% (many diffs)
18 mouse 77626 Smpd4 sphingomyelin phosphodieste... NM_001164611.1 72% 75.9% (many diffs)
19 mouse 77626 Smpd4 sphingomyelin phosphodieste... NM_001164609.1 71% 74.8% (many diffs)
20 mouse 77626 Smpd4 sphingomyelin phosphodieste... XM_006522721.3 69.6% 73.3% (many diffs)
21 mouse 77626 Smpd4 sphingomyelin phosphodieste... XM_006522725.2 65.6% 59.8% (many diffs)
22 mouse 77626 Smpd4 sphingomyelin phosphodieste... XR_001781871.1 53.5% (many diffs)
23 mouse 77626 Smpd4 sphingomyelin phosphodieste... XR_001781872.1 50.5% (many diffs)
Download CSV

Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
66
ORF end:
2271
ORF length:
2205
Sequence:
1tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag
61ttggcatgat gaagttggtt tataagcttc aagctgaaga ctataagttc gactttcctg
121tctcctactt gcctggtcct gtgaaggcgt ccatccagga gtgcatcctc cctgacagtc
181ctctgtacca caacaaggtc cagttcaccc ctactggggg ccttggtctg aacttggccc
241tgaatccgtt cgagtattac atattcttct ttgccttgag cctcatcact cagaagccac
301ttcctgtgtc cctccacgtc cgtacttcag actgtgccta tttcatcctg gtggacaggt
361acctgtcatg gttcctgccc accgaaggca gtgtgccccc accactctcc tccagcccag
421gggggaccag cccctcacca cctcccagga caccagccat accctttgct tcctatggcc
481tccaccacac tagcctccta aagcgacaca tctctcatca gacatctgtg aatgcagacc
541ccgcctccca cgagatctgg aggtcagaaa ctctgctcca ggtttttgtt gaaatgtggc
601ttcatcacta ttccttggag atgtatcaaa aaatgcagtc ccctcatgcc aagctggagg
661ttctgcacta ccgactcagt gtctccagcg ccctctacag ccccgcccaa cccagcctcc
721aggccctcca cgcctaccaa gagtcgttca cgcctactga ggagcatgtg ttggtggtgc
781gcctgctgct gaagcacctg cacgcctttg ccaacagcct gaagccagag caggcctcac
841cctccgccca ctcccacgcc accagccccc tggaggagtt caaacgggct gctgtcccga
901ggttcgtcca gcagaaactc tacctcttct tgcagcattg ctttggccac tggcccctgg
961acgcatcgtt cagagctgtc ctggagatgt ggctgagcta cctgcagccg tggcggtacg
1021cgcctgacaa gcaggctccg ggcagcgact cccagccccg gtgtgtgtcg gagaaatggg
1081caccctttgt ccaggagaac ctgctgatgt acaccaagtt gtttgtgggc tttctgaacc
1141gcgcgctccg cacagacctg gtcagcccca agcacgcgct catggtgttc cgagtggcca
1201aagtctttgc ccagcccaac ctggctgaga tgattcagaa aggtgagcag ctattcctgg
1261agccagagct ggtcatcccc caccgccagc accgactctt cacggccccc acattcactg
1321ggagcttcct gtcaccctgg ccaccagcgg tcactgatgc ctccttcaag gtgaagagcc
1381acgtctacag cctggagggc caggactgca agtacacccc gatgtttggg cccgaggccc
1441gcaccctggt cctgcgcctc gctcagctca tcacacaggc caaacacaca gccaagtcca
1501tctccgacca gtgtgcggag agcccggctg gccactcctt cctctcatgg ctgggcttta
1561gctccatgga caccaatggc tcctacacag ccaacgacct ggacgagatg gggcaagaca
1621gtgtccggaa gacagatgaa tacctggaga aggccctgga gtacctgcgc cagatattcc
1681ggctcagcga agcgcagctc aggcagttca cactcgcctt gggcaccacc caggatgaga
1741atggaaaaaa gcaactcccc gactgcatcg tgggtgagga cggactcatc cttacgcccc
1801tggggcggta ccagatcatc aatgggctgc gaaggtttga aattgagtac cagggggacc
1861cggagctgca gcccatccgg agctatgaga tcgccagctt ggtccgcaca ctctttaggc
1921tgtcgtctgc catcaaccac agatttgcag gacagatggc ggctctgtgt tcccgggatg
1981acttcctcgg cagcttctgt cgctaccacc tcacagaacc tgggctggcc agcaggcacc
2041tgctgagccc tgtggggcgg aggcaggtgg ccggccacac ccgcggcccc aggctcagcc
2101tgcgcttcct gggcagttac cggacgctgg tctcgctgct gctggccttc ttcgtggcct
2161ctctgttcTG CGTCGGGCCC CTCCCATGCA CGCTGCTGCT CACCCTGGGC TATGTCCTCT
2221ACGCCTCTGC CATGACACTG CTGACCGAGC GGGGGAAGCT GCACCAGCCC TGCCCAACTT
2281TCTTGTACAA AGTGGTTGAT ATCGGTAAGC CTATCCCTAA CCCTCTCCTC GGTCTCGATT
2341CTACGTAGTA ATGAACTAGT CCGTAACTTG AAAGTATTTC GATTTCTTGG CTTTATATAT
2401CTTGTGGAAA GGACGACCTT TCAAACCCGA TGTTAGGCCA ACGCGTTAAG TCgacaatca
2461acctctggat tacaaaattt gtgaaagatt

Download FASTA (ORF) (Full)