Construct: ORF TRCN0000477691

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF016667.1_s317c1
Derived from:
ccsbBroadEn_08070
DNA Barcode:
ATACGATCGAGTTAACGTCAACCC
Epitope Tag:
V5
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
DISC1 (27185)

Vector Information:

Vector Backbone:
pLX_317
Pol II Cassette 1:
SV40-PuroR
Pol II Cassette 2:
EF1a-TRCN0000477691
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
V5

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 27185 DISC1 DISC1 scaffold protein NM_001012957.2 99.9% 99.8% 791G>A
2 human 27185 DISC1 DISC1 scaffold protein NM_018662.3 97.3% 97.3% 791G>A;2240_2305del
3 human 27185 DISC1 DISC1 scaffold protein NM_001164537.2 93.8% 93.7% 791G>A;1116_1211del;2336_2401del
4 human 27185 DISC1 DISC1 scaffold protein NM_001164538.2 92.5% 86.9% (many diffs)
5 human 27185 DISC1 DISC1 scaffold protein NM_001164539.2 86.1% 81.3% (many diffs)
6 human 27185 DISC1 DISC1 scaffold protein NM_001164541.2 83.4% 79.4% 791G>A;1980_1981ins61;2085_2086ins350
7 human 27185 DISC1 DISC1 scaffold protein NM_001164540.2 83% 82.9% 791G>A;1268_1269ins366;1874_1939del
8 human 27185 DISC1 DISC1 scaffold protein NM_001164542.2 81.7% 81.6% 791G>A;2043_2043delGins454
9 human 27185 DISC1 DISC1 scaffold protein NM_001012959.2 80.6% 79.1% (many diffs)
10 human 27185 DISC1 DISC1 scaffold protein NM_001164544.1 79.4% 79.2% (many diffs)
11 human 100303453 TSNAX-DISC1 TSNAX-DISC1 readthrough (NM... NR_028398.1 70% (many diffs)
12 human 27185 DISC1 DISC1 scaffold protein NM_001164545.1 69.5% 67.7% 791G>A;1687_1688ins103;1737_1738ins656
13 human 27185 DISC1 DISC1 scaffold protein NM_001164546.2 66.8% 65.9% (many diffs)
14 human 27185 DISC1 DISC1 scaffold protein NM_001164547.2 66.8% 65.9% (many diffs)
15 human 100303453 TSNAX-DISC1 TSNAX-DISC1 readthrough (NM... NR_028397.1 66.6% (many diffs)
16 human 27185 DISC1 DISC1 scaffold protein NM_001164548.1 66.1% 65.1% 791G>A;1632_1633ins158;1653_1654ins685
17 human 27185 DISC1 DISC1 scaffold protein NM_001164549.2 65.4% 65.2% (many diffs)
18 human 100303453 TSNAX-DISC1 TSNAX-DISC1 readthrough (NM... NR_028396.1 63.7% (many diffs)
19 human 100303453 TSNAX-DISC1 TSNAX-DISC1 readthrough (NM... NR_028394.1 61.5% (many diffs)
20 human 100303453 TSNAX-DISC1 TSNAX-DISC1 readthrough (NM... NR_028395.1 58.7% (many diffs)
21 human 27185 DISC1 DISC1 scaffold protein NM_001164551.2 50.8% 50.7% 791G>A;1269_1270ins1227
22 human 27185 DISC1 DISC1 scaffold protein NM_001164550.2 47.5% 45.2% (many diffs)
23 human 27185 DISC1 DISC1 scaffold protein NM_001164553.2 45.8% 44.5% (many diffs)
24 human 27185 DISC1 DISC1 scaffold protein NM_001164552.2 45.7% 45% (many diffs)
25 human 100303453 TSNAX-DISC1 TSNAX-DISC1 readthrough (NM... NR_028400.1 45.2% (many diffs)
26 human 27185 DISC1 DISC1 scaffold protein NM_001164554.2 44.9% 44.7% (many diffs)
27 human 27185 DISC1 DISC1 scaffold protein NM_001012958.2 43.6% 42.5% (many diffs)
28 human 27185 DISC1 DISC1 scaffold protein NM_001164555.1 42.7% 42% 791G>A;1045_1046ins70;1068_1069ins1358
29 human 100303453 TSNAX-DISC1 TSNAX-DISC1 readthrough (NM... NR_028399.1 40.4% (many diffs)
30 human 100303453 TSNAX-DISC1 TSNAX-DISC1 readthrough (NM... NR_028393.1 31.1% (many diffs)
31 human 27185 DISC1 DISC1 scaffold protein NM_001164556.1 23.3% 21.7% (many diffs)
Download CSV

Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
69
ORF end:
2565
ORF length:
2496
Sequence:
1tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag
61ttggcaccat gccaggcggg ggtcctcagg gcgccccagc cgccgccggc ggcggcggcg
121tgagccaccg cgcaggcagc cgggattgct taccacctgc agcgtgcttt cggaggcggc
181ggctggcacg gaggccgggc tacatgagaa gctcgacagg gcctgggatc gggttccttt
241ccccagcagt gggcacactg ttccggttcc caggaggggt gtctggcgag gagtcccacc
301actcggagtc cagggccaga cagtgtggcc ttgactcgag aggcctcttg gtccggagcc
361ctgtttccaa gagtgcagca gcccctactg tgacctctgt gagaggaacc tcggcgcact
421ttgggattca gctcagaggt ggcaccagat tgcctgacag gcttagctgg ccgtgtggcc
481ctgggagtgc tgggtggcag caagagtttg cagccatgga tagttctgag accctggacg
541ccagctggga ggcagcctgc agcgatggag caaggcgtgt ccgggcagca ggctctctgc
601catcagcaga gttgagtagc aacagctgca gccctggctg tggccctgag gtccccccaa
661cccctcctgg ctctcacagt gcctttacct caagctttag ctttattcgg ctctcgcttg
721gctctgccgg ggaacgtgga gaagcagaag gctgcccacc atccagagag gctgagtccc
781attgccagag cccccaggag atgggagcca aagctgccag cttggacggg cctcacgagg
841acccgcgatg tctctctcag cccttcagtc tcttggctac acgggtctct gcagacttgg
901cccaggccgc aaggaacagc tccaggccag agcgtgacat gcattcttta ccagacatgg
961accctggctc ctccagttct ctggatccct cactggctgg ctgtggtggt gatgggagca
1021gcggctcagg ggatgcccac tcttgggaca ccctgctcag gaaatgggag ccagtgctgc
1081gggactgcct gctgagaaac cggaggcaga tggaggtaat atccttaaga ttaaaacttc
1141agaaacttca ggaagatgca gttgagaatg atgattatga taaagctgag acgttacaac
1201aaagattaga agacctggaa caagagaaaa tcagcctgca ctttcaactt ccttcaaggc
1261agccagctct tagcagtttc ctgggtcacc tggcagcaca agtccaggct gccttgcgcc
1321gtggggccac tcagcaggcc agcggagatg acacccacac cccactgaga atggagccga
1381ggctgttgga acccactgct caggacagct tgcacgtgtc catcacgaga cgagactggc
1441ttcttcagga aaagcagcag ctacagaaag aaatcgaagc tctccaagca aggatgtttg
1501tgctggaagc caaagatcaa cagctgagaa gggaaataga ggagcaagag cagcaactcc
1561agtggcaggg ctgcgacctg accccactgg tgggccagct gtccctgggt cagctgcagg
1621aggtcagcaa ggccttgcag gacaccctgg cctcagccgg tcagattccc ttccatgcag
1681agccaccgga aaccataagg agcctccagg aaagaataaa atccctcaac ttgtcactta
1741aagaaatcac tactaaggtg tgtatgagtg agaaattctg cagcaccctg aggaagaaag
1801ttaacgatat tgaaacccaa ctaccagcct tgcttgaagc caaaatgcat gccatatcag
1861gaaaccattt ctggacggct aaagacctca ccgaggagat tagatcatta acatcagaga
1921gagaagggct ggagggactc ctcagcaagc tgttggtgtt gagttccagg aatgtcaaaa
1981agctgggaag tgttaaagaa gattacaaca gactgagaag agaagtggag caccaggaga
2041ctgcctatga aacaagtgtg aaggaaaata ctatgaagta catggaaaca cttaagaata
2101aactgtgcag ctgcaagtgt ccactgcttg ggaaagtgtg ggaagctgac ttggaagctt
2161gtcgattgct tatccagagc cTACAGCTCC AGGAAGCCAG GGGAAGCCTG TCTGTAGAAG
2221ATGAGAGGCA GATGGATGAC TTAGAGGGAG CTGCTCCTCC TATTCCCCCC AGGCTCCACT
2281CCGAGGATAA AAGGAAGACC CCTTTGAAGG AATCTTACAT CCTTTCTGCA GAACTTGGAG
2341AAAAGTGTGA AGACATAGGC AAGAAGCTAT TGTACTTGGA AGATCAACTT CACACAGCAA
2401TCCACAGTCA TGATGAAGAT CTCATTCAGT CTCTCAGGAG GGAGCTCCAG ATGGTGAAGG
2461AAACTCTGCA GGCCATGATC CTGCAGCTCC AGCCAGCAAA GGAGGCGGGA GAAAGAGAAG
2521CTGCAGCTTC CTGCATGACA GCTGGTGTCC ACGAAGCACA AGCCTTGCCA ACTTTCTTGT
2581ACAAAGTGGT TGATATCGGT AAGCCTATCC CTAACCCTCT CCTCGGTCTC GATTCTACGT
2641AGTAATGAAC TAGTCCGTAA CTTGAAAGTA TTTCGATTTC TTGGCTTTAT ATATCTTGTG
2701GAAAGGACGA ATACGATCGA GTTAACGTCA ACCCACGCGT TAAGTCgaca atcaacctct
2761ggattacaaa atttgtgaaa gatt

Download FASTA (ORF) (Full)