Construct: ORF TRCN0000477691
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF016667.1_s317c1
- Derived from:
- ccsbBroadEn_08070
- DNA Barcode:
- ATACGATCGAGTTAACGTCAACCC
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- DISC1 (27185)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000477691
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 27185 | DISC1 | DISC1 scaffold protein | NM_001012957.2 | 99.9% | 99.8% | 791G>A |
| 2 | human | 27185 | DISC1 | DISC1 scaffold protein | NM_018662.3 | 97.3% | 97.3% | 791G>A;2240_2305del |
| 3 | human | 27185 | DISC1 | DISC1 scaffold protein | NM_001164537.2 | 93.8% | 93.7% | 791G>A;1116_1211del;2336_2401del |
| 4 | human | 27185 | DISC1 | DISC1 scaffold protein | NM_001164538.2 | 92.5% | 86.9% | (many diffs) |
| 5 | human | 27185 | DISC1 | DISC1 scaffold protein | NM_001164539.2 | 86.1% | 81.3% | (many diffs) |
| 6 | human | 27185 | DISC1 | DISC1 scaffold protein | NM_001164541.2 | 83.4% | 79.4% | 791G>A;1980_1981ins61;2085_2086ins350 |
| 7 | human | 27185 | DISC1 | DISC1 scaffold protein | NM_001164540.2 | 83% | 82.9% | 791G>A;1268_1269ins366;1874_1939del |
| 8 | human | 27185 | DISC1 | DISC1 scaffold protein | NM_001164542.2 | 81.7% | 81.6% | 791G>A;2043_2043delGins454 |
| 9 | human | 27185 | DISC1 | DISC1 scaffold protein | NM_001012959.2 | 80.6% | 79.1% | (many diffs) |
| 10 | human | 27185 | DISC1 | DISC1 scaffold protein | NM_001164544.1 | 79.4% | 79.2% | (many diffs) |
| 11 | human | 100303453 | TSNAX-DISC1 | TSNAX-DISC1 readthrough (NM... | NR_028398.1 | 70% | (many diffs) | |
| 12 | human | 27185 | DISC1 | DISC1 scaffold protein | NM_001164545.1 | 69.5% | 67.7% | 791G>A;1687_1688ins103;1737_1738ins656 |
| 13 | human | 27185 | DISC1 | DISC1 scaffold protein | NM_001164546.2 | 66.8% | 65.9% | (many diffs) |
| 14 | human | 27185 | DISC1 | DISC1 scaffold protein | NM_001164547.2 | 66.8% | 65.9% | (many diffs) |
| 15 | human | 100303453 | TSNAX-DISC1 | TSNAX-DISC1 readthrough (NM... | NR_028397.1 | 66.6% | (many diffs) | |
| 16 | human | 27185 | DISC1 | DISC1 scaffold protein | NM_001164548.1 | 66.1% | 65.1% | 791G>A;1632_1633ins158;1653_1654ins685 |
| 17 | human | 27185 | DISC1 | DISC1 scaffold protein | NM_001164549.2 | 65.4% | 65.2% | (many diffs) |
| 18 | human | 100303453 | TSNAX-DISC1 | TSNAX-DISC1 readthrough (NM... | NR_028396.1 | 63.7% | (many diffs) | |
| 19 | human | 100303453 | TSNAX-DISC1 | TSNAX-DISC1 readthrough (NM... | NR_028394.1 | 61.5% | (many diffs) | |
| 20 | human | 100303453 | TSNAX-DISC1 | TSNAX-DISC1 readthrough (NM... | NR_028395.1 | 58.7% | (many diffs) | |
| 21 | human | 27185 | DISC1 | DISC1 scaffold protein | NM_001164551.2 | 50.8% | 50.7% | 791G>A;1269_1270ins1227 |
| 22 | human | 27185 | DISC1 | DISC1 scaffold protein | NM_001164550.2 | 47.5% | 45.2% | (many diffs) |
| 23 | human | 27185 | DISC1 | DISC1 scaffold protein | NM_001164553.2 | 45.8% | 44.5% | (many diffs) |
| 24 | human | 27185 | DISC1 | DISC1 scaffold protein | NM_001164552.2 | 45.7% | 45% | (many diffs) |
| 25 | human | 100303453 | TSNAX-DISC1 | TSNAX-DISC1 readthrough (NM... | NR_028400.1 | 45.2% | (many diffs) | |
| 26 | human | 27185 | DISC1 | DISC1 scaffold protein | NM_001164554.2 | 44.9% | 44.7% | (many diffs) |
| 27 | human | 27185 | DISC1 | DISC1 scaffold protein | NM_001012958.2 | 43.6% | 42.5% | (many diffs) |
| 28 | human | 27185 | DISC1 | DISC1 scaffold protein | NM_001164555.1 | 42.7% | 42% | 791G>A;1045_1046ins70;1068_1069ins1358 |
| 29 | human | 100303453 | TSNAX-DISC1 | TSNAX-DISC1 readthrough (NM... | NR_028399.1 | 40.4% | (many diffs) | |
| 30 | human | 100303453 | TSNAX-DISC1 | TSNAX-DISC1 readthrough (NM... | NR_028393.1 | 31.1% | (many diffs) | |
| 31 | human | 27185 | DISC1 | DISC1 scaffold protein | NM_001164556.1 | 23.3% | 21.7% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 2565
- ORF length:
- 2496
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcaccat gccaggcggg ggtcctcagg gcgccccagc cgccgccggc ggcggcggcg 121 tgagccaccg cgcaggcagc cgggattgct taccacctgc agcgtgcttt cggaggcggc 181 ggctggcacg gaggccgggc tacatgagaa gctcgacagg gcctgggatc gggttccttt 241 ccccagcagt gggcacactg ttccggttcc caggaggggt gtctggcgag gagtcccacc 301 actcggagtc cagggccaga cagtgtggcc ttgactcgag aggcctcttg gtccggagcc 361 ctgtttccaa gagtgcagca gcccctactg tgacctctgt gagaggaacc tcggcgcact 421 ttgggattca gctcagaggt ggcaccagat tgcctgacag gcttagctgg ccgtgtggcc 481 ctgggagtgc tgggtggcag caagagtttg cagccatgga tagttctgag accctggacg 541 ccagctggga ggcagcctgc agcgatggag caaggcgtgt ccgggcagca ggctctctgc 601 catcagcaga gttgagtagc aacagctgca gccctggctg tggccctgag gtccccccaa 661 cccctcctgg ctctcacagt gcctttacct caagctttag ctttattcgg ctctcgcttg 721 gctctgccgg ggaacgtgga gaagcagaag gctgcccacc atccagagag gctgagtccc 781 attgccagag cccccaggag atgggagcca aagctgccag cttggacggg cctcacgagg 841 acccgcgatg tctctctcag cccttcagtc tcttggctac acgggtctct gcagacttgg 901 cccaggccgc aaggaacagc tccaggccag agcgtgacat gcattcttta ccagacatgg 961 accctggctc ctccagttct ctggatccct cactggctgg ctgtggtggt gatgggagca 1021 gcggctcagg ggatgcccac tcttgggaca ccctgctcag gaaatgggag ccagtgctgc 1081 gggactgcct gctgagaaac cggaggcaga tggaggtaat atccttaaga ttaaaacttc 1141 agaaacttca ggaagatgca gttgagaatg atgattatga taaagctgag acgttacaac 1201 aaagattaga agacctggaa caagagaaaa tcagcctgca ctttcaactt ccttcaaggc 1261 agccagctct tagcagtttc ctgggtcacc tggcagcaca agtccaggct gccttgcgcc 1321 gtggggccac tcagcaggcc agcggagatg acacccacac cccactgaga atggagccga 1381 ggctgttgga acccactgct caggacagct tgcacgtgtc catcacgaga cgagactggc 1441 ttcttcagga aaagcagcag ctacagaaag aaatcgaagc tctccaagca aggatgtttg 1501 tgctggaagc caaagatcaa cagctgagaa gggaaataga ggagcaagag cagcaactcc 1561 agtggcaggg ctgcgacctg accccactgg tgggccagct gtccctgggt cagctgcagg 1621 aggtcagcaa ggccttgcag gacaccctgg cctcagccgg tcagattccc ttccatgcag 1681 agccaccgga aaccataagg agcctccagg aaagaataaa atccctcaac ttgtcactta 1741 aagaaatcac tactaaggtg tgtatgagtg agaaattctg cagcaccctg aggaagaaag 1801 ttaacgatat tgaaacccaa ctaccagcct tgcttgaagc caaaatgcat gccatatcag 1861 gaaaccattt ctggacggct aaagacctca ccgaggagat tagatcatta acatcagaga 1921 gagaagggct ggagggactc ctcagcaagc tgttggtgtt gagttccagg aatgtcaaaa 1981 agctgggaag tgttaaagaa gattacaaca gactgagaag agaagtggag caccaggaga 2041 ctgcctatga aacaagtgtg aaggaaaata ctatgaagta catggaaaca cttaagaata 2101 aactgtgcag ctgcaagtgt ccactgcttg ggaaagtgtg ggaagctgac ttggaagctt 2161 gtcgattgct tatccagagc cTACAGCTCC AGGAAGCCAG GGGAAGCCTG TCTGTAGAAG 2221 ATGAGAGGCA GATGGATGAC TTAGAGGGAG CTGCTCCTCC TATTCCCCCC AGGCTCCACT 2281 CCGAGGATAA AAGGAAGACC CCTTTGAAGG AATCTTACAT CCTTTCTGCA GAACTTGGAG 2341 AAAAGTGTGA AGACATAGGC AAGAAGCTAT TGTACTTGGA AGATCAACTT CACACAGCAA 2401 TCCACAGTCA TGATGAAGAT CTCATTCAGT CTCTCAGGAG GGAGCTCCAG ATGGTGAAGG 2461 AAACTCTGCA GGCCATGATC CTGCAGCTCC AGCCAGCAAA GGAGGCGGGA GAAAGAGAAG 2521 CTGCAGCTTC CTGCATGACA GCTGGTGTCC ACGAAGCACA AGCCTTGCCA ACTTTCTTGT 2581 ACAAAGTGGT TGATATCGGT AAGCCTATCC CTAACCCTCT CCTCGGTCTC GATTCTACGT 2641 AGTAATGAAC TAGTCCGTAA CTTGAAAGTA TTTCGATTTC TTGGCTTTAT ATATCTTGTG 2701 GAAAGGACGA ATACGATCGA GTTAACGTCA ACCCACGCGT TAAGTCgaca atcaacctct 2761 ggattacaaa atttgtgaaa gatt