Construct: ORF TRCN0000477930
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF015382.1_s317c1
- Derived from:
- ccsbBroadEn_01377
- DNA Barcode:
- AAATCGCTCGATATGCAACCGACA
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- RARG (5916)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000477930
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 5916 | RARG | retinoic acid receptor gamma | NM_000966.6 | 100% | 100% | |
| 2 | human | 5916 | RARG | retinoic acid receptor gamma | XM_024449113.1 | 100% | 100% | |
| 3 | human | 5916 | RARG | retinoic acid receptor gamma | NM_001042728.3 | 91.2% | 88.7% | (many diffs) |
| 4 | human | 5916 | RARG | retinoic acid receptor gamma | NM_001243730.1 | 84.1% | 84.1% | 0_1ins216 |
| 5 | human | 5916 | RARG | retinoic acid receptor gamma | XM_024449114.1 | 84.1% | 84.1% | 0_1ins216 |
| 6 | human | 5916 | RARG | retinoic acid receptor gamma | XM_024449112.1 | 83.9% | 83.9% | 1_261del |
| 7 | human | 5916 | RARG | retinoic acid receptor gamma | NM_001243732.2 | 82.7% | 77.8% | (many diffs) |
| 8 | human | 5916 | RARG | retinoic acid receptor gamma | NM_001243731.2 | 73.3% | 73.3% | 0_1ins363 |
| 9 | mouse | 19411 | Rarg | retinoic acid receptor, gamma | NM_011244.4 | 88.9% | 97.1% | (many diffs) |
| 10 | mouse | 19411 | Rarg | retinoic acid receptor, gamma | XM_006520650.1 | 88.9% | 97.1% | (many diffs) |
| 11 | mouse | 19411 | Rarg | retinoic acid receptor, gamma | XM_011245525.2 | 88.9% | 97.1% | (many diffs) |
| 12 | mouse | 19411 | Rarg | retinoic acid receptor, gamma | NM_001042727.2 | 81.9% | 85.8% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 1431
- ORF length:
- 1362
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcaccat ggccaccaat aaggagcgac tctttgcggc tggtgccctg gggcctggat 121 ctggctaccc aggggcaggt ttccccttcg ccttcccagg ggcactcagg gggtctccgc 181 ctttcgagat gctgagccct agcttccggg gcctgggcca gcctgacctc cccaaggaga 241 tggcctctct gtcggtggag acacagagca ccagctcaga ggagatggtg cccagctcgc 301 cctcgccccc tccgcctcct cgggtctaca agccatgctt cgtgtgcaat gacaagtcct 361 ctggctacca ctatggggtc agctcttgtg aaggctgcaa gggcttcttt cgccgaagca 421 tccagaagaa catggtgtac acgtgtcacc gcgacaaaaa ctgtatcatc aacaaggtga 481 ccaggaatcg ctgccagtac tgccggctac agaagtgctt cgaagtgggc atgtccaagg 541 aagctgtgcg aaatgaccgg aacaagaaga agaaagaggt gaaggaagaa gggtcacctg 601 acagctatga gctgagccct cagttagaag agctcatcac caaggtcagc aaagcccatc 661 aggagacttt cccctcgctc tgccagctgg gcaagtatac cacgaactcc agtgcagacc 721 accgcgtgca gctggatctg gggctgtggg acaagttcag tgagctggct accaagtgca 781 tcatcaagat cgtggagttt gccaagcggt tgcctggctt tacagggctc agcattgctg 841 accagatcac tctgctcaaa gctgcctgcc tagatatcct gatgctgcgt atctgcacaa 901 ggtacacccc agagcaggac accatgacct tctccgacgg gctgaccctg aaccggaccc 961 agatgcacaa tgccggcttc gggcccctca cagaccttgt ctttgccttt gctgggcagc 1021 tcctgcccct ggagatggat gacaccgaga cagggctgct cagcgccatc tgccTCATCT 1081 GCGGAGACCG CATGGACCTG GAGGAGCCCG AAAAAGTGGA CAAGCTGCAG GAGCCACTGC 1141 TGGAAGCCCT GAGGCTGTAC GCCCGGCGCC GGCGGCCCAG CCAGCCCTAC ATGTTCCCAA 1201 GGATGCTAAT GAAAATCACC GACCTCCGGG GCATCAGCAC TAAGGGAGCT GAAAGGGCCA 1261 TTACTCTGAA GATGGAGATT CCAGGCCCGA TGCCTCCCTT AATCCGAGAG ATGCTGGAGA 1321 ACCCTGAAAT GTTTGAGGAT GACTCCTCGC AGCCTGGTCC CCACCCCAAT GCCTCTAGCG 1381 AGGATGAGGT TCCTGGGGGC CAGGGCAAAG GGGGCCTGAA GTCCCCAGCC TTGCCAACTT 1441 TCTTGTACAA AGTGGTTGAT ATCGGTAAGC CTATCCCTAA CCCTCTCCTC GGTCTCGATT 1501 CTACGTAGTA ATGAACTAGT CCGTAACTTG AAAGTATTTC GATTTCTTGG CTTTATATAT 1561 CTTGGGAAAG GACGAAAATC GCTCGATATG CAACCGACAA CGCGTTAAGT Cgacaatcaa 1621 cctctggatt acaaaatttg tgaaagatt