Construct: ORF TRCN0000477946

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF005662.1_s317c1
Derived from:
ccsbBroadEn_08879
DNA Barcode:
TTTTGACAAAGTAGCTATTCAATA
Epitope Tag:
V5
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
CASD1 (64921)

Vector Information:

Vector Backbone:
pLX_317
Pol II Cassette 1:
SV40-PuroR
Pol II Cassette 2:
EF1a-TRCN0000477946
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
V5

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 64921 CASD1 CAS1 domain containing 1 NM_022900.5 99.8% 99.8% 1156C>A;1464T>C;1857T>C
2 human 64921 CASD1 CAS1 domain containing 1 XM_011516495.2 90.1% 88.8% (many diffs)
3 human 64921 CASD1 CAS1 domain containing 1 NM_001363426.1 81.9% 81.9% (many diffs)
4 human 64921 CASD1 CAS1 domain containing 1 NM_001363428.1 76.6% 76.6% (many diffs)
5 human 64921 CASD1 CAS1 domain containing 1 NM_001363427.1 65.2% 64.6% (many diffs)
6 human 64921 CASD1 CAS1 domain containing 1 XM_017012546.2 65.2% 64.6% (many diffs)
7 human 64921 CASD1 CAS1 domain containing 1 XM_006716093.3 58.2% 57.2% (many diffs)
8 mouse 213819 Casd1 CAS1 domain containing 1 NM_145398.2 89.6% 94.7% (many diffs)
9 mouse 213819 Casd1 CAS1 domain containing 1 XM_006505029.2 73.3% 77.3% (many diffs)
10 mouse 213819 Casd1 CAS1 domain containing 1 XM_006505030.2 64.4% 67.5% (many diffs)
Download CSV

Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
66
ORF end:
2457
ORF length:
2391
Sequence:
1tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag
61ttggcatggc ggctctggcc tacaacctgg gcaagcggga gatcaaccac tacttcagcg
121tgaggagcgc caaggtgctg gcgctggtgg ccgtgctgct gctcgcagcg tgccacctcg
181cctcccgccg ctaccgaggc aatgattcgt gtgaatacct tctctcaagt ggcagatttc
241ttggagagaa agtttggcaa cctcacagtt gtatgatgca taaatacaaa atcagtgaag
301caaagaactg ccttgtagat aaacatattg catttattgg agattccaga attcgtcaat
361tgttttattc ttttgtaaaa ataattaatc cccaattcaa agaagaagga aataagcatg
421aaaacattcc ttttgaagac aagactgcat cagttaaagt ggattttctg tggcatcctg
481aagttaatgg ttctatgaaa cagtgtatca aagtgtggac tgaggattcc attgcaaagc
541cacatgtgat tgtagcagga gctgccacat ggtccatcaa gattcacaat ggtagcagtg
601aagcgctttc tcaatataaa atgaacatca cctccatagc accactttta gaaaaattgg
661caaagactag tgatgtttat tgggtcttac aagatcctgt ttatgaagat ctattaagtg
721aaaataggaa gatgatcact aatgagaaga tagatgctta caatgaagct gcagtcagta
781ttttgaatag tagcaccaga aattctaaat caaatgttaa gatgttcagt gtttccaaat
841taattgctca agaaaccatc atggaatctt tggatggctt acatcttcct gaatcgagca
901gagaaactac tgcaatgatt cttatgaatg tgtattgcaa taagattttg aagcctgtag
961atgggtcctg ttgtcaacct cggcctcctg ttactctcat acagaagcta gctgcttgtt
1021ttttcacttt atctattatc ggatatttaa ttttttacat aattcatcgt aatgctcatc
1081ggaagaataa gccgtgtact gatttggaaa gtggagagga aaagaaaaat attatcaata
1141cccctgtgtc ttcattagaa atacttttac aatctttctg caaacttggc ctgattatgg
1201catatttcta tatgtgtgac agtgcaaatc tgttcatgaa ggaaaacaaa ttttatacac
1261attcatcttt ctttattcca attatctaca ttttggtttt gggagtattt tataatgaaa
1321atactaaaga gactaaagta ttaaatagag aacaaacaga cgaatggaaa ggctggatgc
1381aacttgtgat tttgatttat cacatttctg gagcaagtac atttttgcct gtatacatgc
1441acattcgagt tctggttgct gcatatttat ttcagacagg gtatgggcat ttctcatact
1501tttggataaa aggagatttt ggaatctaca gagtatgtca ggttttattt cgtctcaatt
1561tcctggtagt ggtgttatgt atagtaatgg atcgacctta tcaattctat tactttgtcc
1621ccttggtcac tgtatggttc atggtcatat atgttacttt agcactatgg ccacaaataa
1681tccaaaaaaa agcaaacgga aattgtttct ggcattttgg cttactgttg aaactaggct
1741ttttgctgtt attcatatgt tttttggcat attctcaggg tgcatttgag aagatctttt
1801ctctttggcc attgtccaag tgttttgaac tgaaagggaa tgtatatgaa tggtggttca
1861gatggaggtt agaccgttat gtagttttcc acggaatgct gtttgctttt atttatctgg
1921ccttgcagaa gcgtcaaata ctttctgaag gaaagggtga acctcttttt tcaaacaaaa
1981tttcaaattt tctgttgttt atttcagtag tttctttctt gacctattcc atctgggcta
2041gcagttgtaa aaacaaagca gagtgcaatg aactccatcc gtctgtttct gtggtacaga
2101ttttagcctt catcctaata agaaacatcc ctggatatgc ccgttcagtt tacagttcat
2161tttttgcttg gtttGGAAAA ATTTCATTAG AGCTATTTAT TTGCCAGTAT CACATATGGC
2221TGGCAGCGGA CACAAGGGGT ATCTTGGTAC TGATACCTGG AAACCCTATG CTCAACATCA
2281TTGTCAGCAC TTTCATATTT GTTTGTGTGG CACATGAAAT TTCTCAGATC ACTAATGATC
2341TTGCACAGAT TATTATTCCT AAAGATAACT CATCTCTCTT GAAAAGGTTG GCATGTATAG
2401CTGCATTTTT TTGTGGACTC CTCATCTTAT CATCCATTCA AGATAAATCA AAACATTACC
2461CAACTTTCTT GTACAAAGTG GTTGATATCG GTAAGCCTAT CCCTAACCCT CTCCTCGGTC
2521TCGATTCTAC GTAGTAATGA ACTAGTCCGT AACTTGAAAG TATTTCGATT TCTTGGCTTT
2581ATATATCTTG TGGAAAGGAC GATTTTGACA AAGTAGCTAT TCAATAACGC GTTAAGTCga
2641caatcaacct ctggattaca aaatttgtga aagatt

Download FASTA (ORF) (Full)