Transcript: Human XM_017012546.2

PREDICTED: Homo sapiens CAS1 domain containing 1 (CASD1), transcript variant X5, mRNA.

Source:
NCBI, updated 2019-09-08
Taxon:
Homo sapiens (human)
Gene:
CASD1 (64921)
Length:
3454
CDS:
673..2244

Additional Resources:

NCBI RefSeq record:
XM_017012546.2
NBCI Gene record:
CASD1 (64921)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Human XM_017012546.2, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Human Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000417982 AGACTGTCTGCACCTGTATTC pLKO_005 2645 3UTR 100% 10.800 15.120 N CASD1 n/a
2 TRCN0000420817 TAGTAATGGATCGACCTTATC pLKO_005 1367 CDS 100% 10.800 15.120 N CASD1 n/a
3 TRCN0000035666 CCCGTTCAGTTTACAGTTCAT pLKO.1 1925 CDS 100% 4.950 6.930 N CASD1 n/a
4 TRCN0000035664 CGGAAATTGTTTCTGGCATTT pLKO.1 1482 CDS 100% 10.800 8.640 N CASD1 n/a
5 TRCN0000035665 CGAGGCAATGATTCGTGTGAA pLKO.1 195 5UTR 100% 4.950 3.960 N CASD1 n/a
6 TRCN0000416130 AGTTCTGGTTGCTGCATATTT pLKO_005 1233 CDS 100% 15.000 10.500 N CASD1 n/a
7 TRCN0000428880 ATGATCTTGCACAGATTATTA pLKO_005 2120 CDS 100% 15.000 10.500 N CASD1 n/a
8 TRCN0000414174 CATCTATCTGGAGATATAAAT pLKO_005 2310 3UTR 100% 15.000 10.500 N CASD1 n/a
9 TRCN0000428103 ATGAAGGCAGGGAAATGATTA pLKO_005 2542 3UTR 100% 13.200 9.240 N CASD1 n/a
10 TRCN0000035668 GCTGGATGCAACTTGTGATTT pLKO.1 1157 CDS 100% 13.200 9.240 N CASD1 n/a
11 TRCN0000421353 GGCTTTGCAGAAGCGTCAAAT pLKO_005 1704 CDS 100% 13.200 9.240 N CASD1 n/a
12 TRCN0000121046 CTGCATCAGTTAAAGTGGATT pLKO.1 445 5UTR 100% 4.950 3.465 N Casd1 n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript XM_017012546.2, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_08879 pDONR223 100% 65.2% 64.6% None (many diffs) n/a
2 ccsbBroad304_08879 pLX_304 0% 65.2% 64.6% V5 (many diffs) n/a
3 TRCN0000477946 TTTTGACAAAGTAGCTATTCAATA pLX_317 12.1% 65.2% 64.6% V5 (many diffs) n/a
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