Construct: ORF TRCN0000477986
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF014217.1_s317c1
- Derived from:
- ccsbBroadEn_14032
- DNA Barcode:
- TCTGCATGAAGTGAAGCCCGGGGC
- Epitope Tag:
- V5 (not translated due to prior stop codon)
- Notes:
- Early stop codon detected
Originally Annotated References:
- Gene:
- SGSM2 (9905)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000477986
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 9905 | SGSM2 | small G protein signaling m... | NM_014853.3 | 99.6% | 16.9% | (many diffs) |
| 2 | human | 9905 | SGSM2 | small G protein signaling m... | XM_011524106.1 | 98.5% | 16.8% | (many diffs) |
| 3 | human | 9905 | SGSM2 | small G protein signaling m... | XM_011524102.3 | 95.5% | 16.1% | (many diffs) |
| 4 | human | 9905 | SGSM2 | small G protein signaling m... | NM_001098509.2 | 95.4% | 17.6% | (many diffs) |
| 5 | human | 9905 | SGSM2 | small G protein signaling m... | XM_011524103.3 | 94.9% | 16.2% | (many diffs) |
| 6 | human | 9905 | SGSM2 | small G protein signaling m... | XM_011524101.3 | 94.4% | 16% | (many diffs) |
| 7 | human | 9905 | SGSM2 | small G protein signaling m... | XM_011524108.1 | 94.3% | 17.5% | (many diffs) |
| 8 | human | 9905 | SGSM2 | small G protein signaling m... | NM_001346700.2 | 93% | 18% | (many diffs) |
| 9 | human | 9905 | SGSM2 | small G protein signaling m... | XM_011524104.2 | 92.8% | 14.3% | (many diffs) |
| 10 | human | 9905 | SGSM2 | small G protein signaling m... | XM_017025474.2 | 91.4% | 16.8% | (many diffs) |
| 11 | human | 9905 | SGSM2 | small G protein signaling m... | XM_017025475.2 | 90.8% | 16.9% | (many diffs) |
| 12 | human | 9905 | SGSM2 | small G protein signaling m... | XM_011524105.3 | 90.3% | 16.7% | (many diffs) |
| 13 | human | 9905 | SGSM2 | small G protein signaling m... | XM_011524107.3 | 88.9% | 16.9% | (many diffs) |
| 14 | human | 9905 | SGSM2 | small G protein signaling m... | XM_017025476.1 | 72.9% | 5% | (many diffs) |
| 15 | human | 9905 | SGSM2 | small G protein signaling m... | XR_002958096.1 | 54.4% | (many diffs) | |
| 16 | human | 9905 | SGSM2 | small G protein signaling m... | XR_934127.3 | 51.9% | (many diffs) | |
| 17 | human | 9905 | SGSM2 | small G protein signaling m... | XR_934129.3 | 37.9% | (many diffs) | |
| 18 | mouse | 97761 | Sgsm2 | small G protein signaling m... | XM_006534581.3 | 88.6% | 16% | (many diffs) |
| 19 | mouse | 97761 | Sgsm2 | small G protein signaling m... | NM_197943.2 | 84.4% | 16.7% | (many diffs) |
| 20 | mouse | 97761 | Sgsm2 | small G protein signaling m... | XM_006534583.3 | 80.7% | 14% | (many diffs) |
| 21 | mouse | 97761 | Sgsm2 | small G protein signaling m... | XM_006534584.3 | 78.9% | 15.6% | (many diffs) |
| 22 | mouse | 97761 | Sgsm2 | small G protein signaling m... | XM_006534585.3 | 71.6% | 5.1% | (many diffs) |
| 23 | mouse | 97761 | Sgsm2 | small G protein signaling m... | XM_006534587.2 | 68.2% | 5.3% | (many diffs) |
| 24 | mouse | 97761 | Sgsm2 | small G protein signaling m... | XM_006534589.3 | 57.4% | 6.3% | (many diffs) |
| 25 | mouse | 97761 | Sgsm2 | small G protein signaling m... | XM_017314863.1 | 57.4% | 6.3% | (many diffs) |
| 26 | mouse | 97761 | Sgsm2 | small G protein signaling m... | XM_017314864.1 | 57.4% | 6.3% | (many diffs) |
| 27 | mouse | 97761 | Sgsm2 | small G protein signaling m... | XR_388560.3 | 52.1% | (many diffs) | |
| 28 | mouse | 97761 | Sgsm2 | small G protein signaling m... | XR_001780082.1 | 51.8% | (many diffs) | |
| 29 | mouse | 97761 | Sgsm2 | small G protein signaling m... | XR_001780084.1 | 51.7% | (many diffs) | |
| 30 | mouse | 97761 | Sgsm2 | small G protein signaling m... | XR_001780085.1 | 49.6% | (many diffs) | |
| 31 | mouse | 97761 | Sgsm2 | small G protein signaling m... | XR_001780083.1 | 49.3% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 618
- ORF length:
- 552
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatggg cagcgcagag gacgcagtca aagagaaact gctgtggaac gtgaagaagg 121 aggtgaagca aatcatggag gaggctgtca ccaggaagtt tgtgcatgaa gacagcagcc 181 acatcattgc tttatgtggt gcagtggagg cttgcctctt gcatcagctg agacgccgtg 241 ccgctggctt catgcgcagt gacaagatgg cagccctgtt caccaaggtg gggaagacgt 301 gcccagtggc gggggagatt tgccacaagg tacaggagct gcagcaacaa gcagagggca 361 ggaaaccctc aggggtcagc caggaggccc tgcggagaca gggctcagcc agcgggaagg 421 ccccggccct cagccctcag gccttgaaac acgtatgggt acgcacggcg ctcatcgaga 481 aagttctgga caaggtcgtg caatacctgg cggaaaactg cagcaagtac tacgagaagg 541 aggcactgct ggcagaccct gtgttcggcc cgatcctggc ctctcttcta gtgggaccct 601 ggtgccttgg aatacactaa gctcaagaca gccgatcact actggactga cccctctgct 661 gatgagctgg tccagcggca ccgcatccgg ggtccaccta ctcgccagga ctcccctgca 721 aagcgcccag ccctggggat ccggaaacgg cactcaagcg gcagcgcgtc ggaggacaag 781 ctggctgcct gcgccagcga gtgtgtggag tccctgcacc agaactcacg gacgcggctg 841 ctctatggca agaaccacgt gctggtgcag ccgaaggagg atatggaggc ggtccctggc 901 tacctctccc tgcaccagtc tgcagagagc ctgactctga agtggacccc caaccagctc 961 atgaatggga ctctggggga ctccgagctg gaaaagagcg tttactggga ctatgccctc 1021 gtggtgccct tcagccaggt cgtgtgcatc cgctgccacc agcaaaagag cggtggcacg 1081 cttgtgctgg tgagccagga tggcatccag aggccgccgc tgcatttccc acagggagga 1141 cacctgctgt cctttctgtc ctgtctggag aatgggctgc tgcctcaggg acagctagag 1201 cccccgctgt ggacccagca agggaagggg aaagtgttcc ccaagctacg gaaacgaagc 1261 agcattcgct ccgtggatat ggaggagatg ggcacggggc gggccaccga ctatgtgttc 1321 cggatcatct accccggcca caggcacgag cacatcacta ttaactacca ccacctagcg 1381 gccagccgcg cggcctcggt ggacgatgat gaggaagagg aggataaact gcacgcgatg 1441 ctctcaatga tctgctcgcg gaacctcaca gctcccaatc cgatgaaaga cgctggtgac 1501 atgatcgaga tgcagggctt tgggcccagc ctgccagcct ggcacctgga gcccctgtgc 1561 agtcagggct cctcctgcct ctcctgctcc tccagcagct ccccacatgc aacccccagc 1621 cactgtagct gcatccccga ccggttgccg ctcaggctac tgtgtgagag tatgaagagg 1681 cagatcgtgt cccgggcctt ctacggctgg ctggcacact gccgccacct gtccacggtg 1741 cggacccacc tgtcggcgct ggtgcaccat agcgttatcc cacctgaccg gcccccgggg 1801 gcctccgcgg gcctcaccaa ggacgtgtgg agcaagtatc agaaggacaa aaagaactac 1861 aaagagctgg agctgctgcg gcaagtttac tacggaggca tagagcacga gatccgcaag 1921 gacgtctggc cctttctgct tggccactac aagttcggca tgagcaagaa ggagatggag 1981 caggtggacg cagtggtggc agcaaggtac cagcaggtgt tggcagagtg gaaggcctgc 2041 gaggtggtgg tgaggcagcg ggagcgggag gcccacccag ccacacgcac caagttctcc 2101 tcaggcagca gcatcgacag ccacgtgcag cgcctcatcc accgagactc caccatcagc 2161 aacgatgtgt ttatctcagt ggatgatctg gaacccccgg agccccagga ccctgaagat 2221 tcaagaccaa aacctgagca ggaagcagga cccgggactc cgggcaccgc cgtggtggag 2281 cagcagcatt ccgtggagtt cgactctcca gactcaggac tgccctcctc tcgcaattac 2341 tccgtggcct cgggcatcca gtcaagccta gatgaggggc agagcgtggg cttcgaagag 2401 gaggacggcg gtggggagga aggctccagt gggcccggcc ctgcagctca cactttgagg 2461 gagccccagg atcccagcca ggagaagcct caggccggag aactggaggc cggagaggag 2521 cttgcggctg tgtgtgcggc tgcctacact atagaattac tggatactgt ggccttaaac 2581 ctgcaccgca tagacaagga tgtgcagagg tgtgaccgca actactggta cttcacgccc 2641 cccaacctcg agaggctcag agacgtcatg tgcagctacg tgtgggagca cctggacgtg 2701 ggctatgtgc agggcatgtg cgatctgctg gcgcctctcc tggtcaccct cgacaatgat 2761 cagctggcct acagctgctt cagccacctc atgaagagga tgagccagaa cttccccaac 2821 gggggtgcca tggacaccca ctttgccaac atgcgctccc tcatccagat cctggactca 2881 gagctgtttg agctgatgca tcagaatgga gactacaccc acttctactt ctgttatcGC 2941 TGGTTCCTGC TGGATTTTAA GAGAGAACTG CTGTATGAGG ATGTGTTTGC TGTGTGGGAG 3001 GTGATCTGGG CAGCCAGGCA CATCTCATCG GAGCACTTTG TCCTGTTCAT CGCCCTCGCC 3061 CTGGTGGAGG CCTACCGAGA GATCATCCGT GACAACAACA TGGTCTTCAC TGACATCATC 3121 AAGTTTTTCA ATGAACGTGC TGAGCATCAC GATGCCCAGG AGATCCTGCG GATTGCCCGG 3181 GACCTCGTCC ACAAGGTGCA GATGCTCATA GAGAACAAGT GCCCAACTTT CTTGTACAAA 3241 GTGGTTGATA TCGGTAAGCC TATCCCTAAC CCTCTCCTCG GTCTCGATTC TACGTAGTAA 3301 TGAACTAGTC CGTAACTTGA AAGTATTTCG ATTTCTTGGC TTTATATATC TTGTGGAAAG 3361 GACGATCTGC ATGAAGTGAA GCCCGGGGCA CGCGTTAAGT Cgacaatcaa cctctggatt 3421 acaaaatttg tgaaagatt