Construct: ORF TRCN0000478101
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF017702.1_s317c1
- Derived from:
- ccsbBroadEn_06249
- DNA Barcode:
- CATCGACAATAGGTAGTCAAACGT
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- GAGE4 (2576)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000478101
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 2543 | GAGE1 | G antigen 1 | NM_001040663.2 | 99.7% | 99.1% | 343T>A |
| 2 | human | 2576 | GAGE4 | G antigen 4 | NM_001474.1 | 99.1% | 98.2% | 337_338delAAinsGG;343T>A |
| 3 | human | 100008586 | GAGE12F | G antigen 12F | NM_001098405.2 | 98.8% | 97.4% | 223C>G;337_338delAAinsGG;343T>A |
| 4 | human | 645073 | GAGE12G | G antigen 12G | NM_001098409.2 | 98.8% | 97.4% | 223C>G;337_338delAAinsGG;343T>A |
| 5 | human | 729428 | GAGE12B | G antigen 12B | NM_001127345.2 | 98.8% | 97.4% | 223C>G;337_338delAAinsGG;343T>A |
| 6 | human | 2577 | GAGE5 | G antigen 5 | NM_001475.1 | 98.8% | 97.4% | 67G>A;337_338delAAinsGG;343T>A |
| 7 | human | 26748 | GAGE12I | G antigen 12I | NM_001477.1 | 98.8% | 97.4% | 223C>G;337_338delAAinsGG;343T>A |
| 8 | human | 2579 | GAGE7 | G antigen 7 | NM_021123.3 | 98.8% | 97.4% | 223C>G;337_338delAAinsGG;343T>A |
| 9 | human | 729396 | GAGE12J | G antigen 12J | NM_001098406.2 | 98.5% | 97.4% | (many diffs) |
| 10 | human | 729422 | GAGE12C | G antigen 12C | NM_001098408.1 | 98.5% | 96.5% | (many diffs) |
| 11 | human | 729431 | GAGE12E | G antigen 12E | NM_001098418.1 | 98.5% | 96.5% | (many diffs) |
| 12 | human | 100132399 | GAGE12D | G antigen 12D | NM_001127199.1 | 98.5% | 96.5% | (many diffs) |
| 13 | human | 2578 | GAGE6 | G antigen 6 | NM_001476.1 | 98.5% | 96.5% | (many diffs) |
| 14 | human | 645051 | GAGE13 | G antigen 13 | NM_001098412.2 | 98.2% | 97.4% | (many diffs) |
| 15 | human | 729442 | GAGE12H | G antigen 12H | NM_001098410.1 | 98.2% | 95.7% | (many diffs) |
| 16 | human | 729408 | GAGE2D | G antigen 2D | NM_001098407.1 | 97.1% | 95.7% | (many diffs) |
| 17 | human | 26749 | GAGE2E | G antigen 2E | NM_001127200.3 | 97.1% | 95.7% | (many diffs) |
| 18 | human | 100101629 | GAGE8 | G antigen 8 | NM_012196.1 | 97.1% | 95.7% | (many diffs) |
| 19 | human | 645037 | GAGE2B | G antigen 2B | NM_001098411.3 | 96.8% | 94.8% | (many diffs) |
| 20 | human | 2574 | GAGE2C | G antigen 2C | NM_001472.2 | 96.8% | 94.8% | (many diffs) |
| 21 | human | 729447 | GAGE2A | G antigen 2A | NM_001127212.1 | 96.5% | 94% | (many diffs) |
| 22 | human | 102724473 | GAGE10 | G antigen 10 | NM_001098413.3 | 94.5% | 89.7% | (many diffs) |
| 23 | human | 2543 | GAGE1 | G antigen 1 | XM_011543896.2 | 90.3% | 94.1% | 333_334insGAAGGGCAAACACAG;337_357del |
| 24 | human | 102724473 | GAGE10 | G antigen 10 | XM_024452325.1 | 79.7% | 71.7% | (many diffs) |
| 25 | human | 645073 | GAGE12G | G antigen 12G | NM_001354422.1 | 63.5% | 61.5% | (many diffs) |
| 26 | human | 2543 | GAGE1 | G antigen 1 | NR_102272.1 | 11% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 420
- ORF length:
- 351
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcaccat gagttggcga ggaagatcga cctattattg gcctagacca aggcgctatg 121 tacagcctcc tgaaatgatt gggcctatgc ggcccgagca gttcagtgat gaagtggaac 181 cagcaacacc tgaagaaggg gaaccagcaa ctcaacgtca ggatcctgca gctgctcagg 241 agggagagga tgagggagca tctgcaggtc aagggccGAA GCCTGAAGCT GATAGCCAGG 301 AACAGGGTCA CCCACAGACT GGGTGTGAGT GTGAAGATGG TCCTGATGGG CAGGAGATGG 361 ACCCGCCAAA TCCAGAGGAG GTGAAAACGC CTGAAGAAGG TGAAGGGCAA ACACAGTGTT 421 TGCCAACTTT CTTGTACAAA GTGGTTGATA TCGGTAAGCC TATCCCTAAC CCTCTCCTCG 481 GTCTCGATTC TACGTAGTAA TGAACTAGTC CGTAACTTGA AAGTATTTCG ATTTCTTGGC 541 TTTATATATC TTGTGGAAAG GACGACATCG ACAATAGGTA GTCAAACGTA CGCGTTAAGT 601 Cgacaatcaa cctctggatt acaaaatttg tgaaagatt