Construct: ORF TRCN0000478142
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF004245.1_s317c1
- Derived from:
- ccsbBroadEn_13444
- DNA Barcode:
- GAGAGTCATAAAAGACCTGAGCGT
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- AKR7L (246181)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000478142
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 246181 | AKR7L | aldo-keto reductase family ... | NM_001348421.1 | 46.2% | 32.3% | 1_337del;508_704del |
| 2 | human | 22977 | AKR7A3 | aldo-keto reductase family ... | NM_012067.3 | 44.9% | 30.8% | (many diffs) |
| 3 | human | 22977 | AKR7A3 | aldo-keto reductase family ... | XM_017000714.2 | 42.6% | 18.9% | (many diffs) |
| 4 | human | 8574 | AKR7A2 | aldo-keto reductase family ... | NM_001320979.1 | 40.8% | 30.5% | (many diffs) |
| 5 | human | 8574 | AKR7A2 | aldo-keto reductase family ... | NM_003689.3 | 40.2% | 28.4% | (many diffs) |
| 6 | human | 22977 | AKR7A3 | aldo-keto reductase family ... | XM_011541046.3 | 34.7% | 15.4% | (many diffs) |
| 7 | human | 8574 | AKR7A2 | aldo-keto reductase family ... | XR_946785.2 | 34.4% | (many diffs) | |
| 8 | human | 246182 | AKR7A2P1 | aldo-keto reductase family ... | NR_002796.2 | 32.6% | (many diffs) | |
| 9 | human | 22977 | AKR7A3 | aldo-keto reductase family ... | XR_001737055.1 | 26% | (many diffs) | |
| 10 | human | 246181 | AKR7L | aldo-keto reductase family ... | NR_040289.2 | 21% | 1_385del;845_2183del | |
| 11 | human | 246181 | AKR7L | aldo-keto reductase family ... | NR_040288.2 | 19.2% | 1_385del;556_752del;1042_2380del | |
| 12 | mouse | 110198 | Akr7a5 | aldo-keto reductase family ... | NM_025337.3 | 36.6% | 25.4% | (many diffs) |
| 13 | mouse | 110198 | Akr7a5 | aldo-keto reductase family ... | XR_390721.3 | 35.8% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 528
- ORF length:
- 459
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcaccat gcacctgacc acagcgcccc ggtggaagag acactgcgtg cctgccacca 121 gctgcaccag gagggcaagt tcgtggagct tggcctctcc aactatgccg cctgggaagt 181 ggccgagatc tgtacccTCT GCAAGAGCAA CGGCTGGATC CTGCCCACTG TGTACCAGCT 241 TCTGGAAGGA GCACCACTTC GAGGGCATTG CCCTGGTGGA GAAGGCCCTG CAGGCCGCGT 301 ATGGCGCCAG CGCTCCCAGC ATGACCTCGG CCGCCCTCCG GTGGATGTAC CACCACTCAC 361 AGCTGCAGGG TGCCCACGGG GACGCGGTCA TCCTGGGCAT GTCCAGCCTG GAGCAGCTGG 421 AGCAGAACTT GGCAGCGGCA GAGGAAGGGC CCCTGGAGCC GGCTGTCGTG GACGCCTTTA 481 ATCAAGCCTG GCATTTGTTT GCCCACGAAT GTCCCAACTA CTTCATCTTG CCAACTTTCT 541 TGTACAAAGT GGTTGATATC GGTAAGCCTA TCCCTAACCC TCTCCTCGGT CTCGATTCTA 601 CGTAGTAATG AACTAGTCCG TAACTTGAAA GTATTTCGAT TTCTTGGCTT TATATATCTT 661 GTGGAAAGGA CGAGAGAGTC ATAAAAGACC TGAGCGTACG CGTTAAGTCg acaatcaacc 721 tctggattac aaaatttgtg aaagatt