Construct: ORF TRCN0000478206
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF011147.1_s317c1
- Derived from:
- ccsbBroadEn_11339
- DNA Barcode:
- TCCATAGACGACGTCGATTACTTT
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- PRPF4 (9128)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000478206
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 9128 | PRPF4 | pre-mRNA processing factor 4 | NM_001244926.1 | 100% | 100% | |
| 2 | human | 9128 | PRPF4 | pre-mRNA processing factor 4 | NM_004697.4 | 99.8% | 99.8% | 27_29delGCA |
| 3 | human | 9128 | PRPF4 | pre-mRNA processing factor 4 | NM_001322266.1 | 52.6% | 52.2% | (many diffs) |
| 4 | human | 9128 | PRPF4 | pre-mRNA processing factor 4 | NM_001322267.1 | 52.6% | 52.2% | (many diffs) |
| 5 | human | 9128 | PRPF4 | pre-mRNA processing factor 4 | NR_136266.1 | 51.8% | 1_110del;1203_1204ins49;1625_2872del | |
| 6 | human | 9128 | PRPF4 | pre-mRNA processing factor 4 | NR_136265.1 | 51.7% | (many diffs) | |
| 7 | mouse | 70052 | Prpf4 | pre-mRNA processing factor 4 | NM_027297.3 | 90.9% | 99% | (many diffs) |
| 8 | mouse | 70052 | Prpf4 | pre-mRNA processing factor 4 | XM_006538249.2 | 48.5% | 51.7% | (many diffs) |
| 9 | mouse | 70052 | Prpf4 | pre-mRNA processing factor 4 | XR_390341.1 | 47.6% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1629
- ORF length:
- 1563
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatggc ttcctcgcga gcctcttcca cggcaaccaa aactaaagca cccgacgact 121 tagttgctcc ggtcgtgaag aaaccacaca tctattatgg aagtttggaa gagaaggaga 181 gggagcgtct ggccaaagga gagtctggga ttttggggaa agacggactt aaagcaggga 241 tcgaagctgg aaatattaat ataacctctg gagaagtgtt tgaaattgaa gagcatatca 301 gcgagcgaca ggcagaagta ttggctgagt ttgagagaag gaagcgagcc cggcagatca 361 atgtttccac agatgactca gaggtcaaag cttgccttag agccttgggg gaacccatca 421 cactttttgg agagggtcct gctgaaagaa gagaaaggtt aagaaatatc ctctcagttg 481 tcggtactga tgccttgaaa aagaccaaaa aggatgatga gaagtctaaa aagtccaaag 541 aagagtatca gcaaacctgg tatcatgaag gaccaaatag cttgaaggtg gcaagactat 601 ggattgctaa ttattcgttg cccagggcaa tgaaacgctt ggaagaggcc cgactccata 661 aggagattcc tgagacaaca aggacctccc agatgcaaga gctgcacaag tctctccggt 721 ctttgaataa tttttgcagt cagattgggg atgatcggcc tatctcctac tgtcacttta 781 gtcccaattc caagatgctg gccacagctt gttggagtgg gctttgcaag ctctggtctg 841 ttcctgattg caacctcctt cacactcttc gagggcataa cacaaatgta ggagcaattg 901 tattccatcc caaatccact gtctccttgg acccaaaaga tgtcaacctg gcctcttgtg 961 cggctgatgg ctctgtgaag ctttggagtc tcgacagtga tgaaccagtg gcagatattg 1021 aaggccatac agtgcgtgtg gcgcgggtaa tgtggcatcc ttcaggacgt ttcctgggca 1081 ccacctgcta tgaccgttca tggcgcttat gggatttgga ggctcaagag gagatcctgc 1141 atcaggaagg ccatagcatg ggtgtgtatg acattgcctt ccatcaagat ggctctttgg 1201 ctggcactgg gggactggat gcatttggtc gagtttggga cctacgcaca ggacgttgta 1261 tcatgttctt agaaggccac ctgaaagaaa tctatggaat aaatttctcc cccaatggct 1321 atcacattgc aaccggcagt ggtgacaaca cctgcaaagt gtgggaccTC CGACAGCGGC 1381 GTTGCGTCTA CACCATCCCT GCTCATCAGA ACTTAGTGAC TGGTGTCAAG TTTGAGCCTA 1441 TCCATGGGAA CTTCTTGCTT ACTGGTGCCT ATGATAACAC AGCCAAGATC TGGACGCACC 1501 CAGGCTGGTC CCCGCTGAAG ACTCTGGCTG GCCACGAAGG CAAAGTGATG GGCCTAGATA 1561 TTTCTTCCGA TGGGCAGCTC ATAGCCACTT GCTCATATGA CAGGACCTTC AAGCTGTGGA 1621 TGGCTGAATA CCCAACTTTC TTGTACAAAG TGGTTGATAT CGGTAAGCCT ATCCCTAACC 1681 CTCTCCTCGG TCTCGATTCT ACGTAGTAAT GAACTAGTCC GTAACTTGAA AGTATTTCGA 1741 TTTCTTGGCT TTATATATCT TGTGGAAAGG ACGATCCATA GACGACGTCG ATTACTTTAC 1801 GCGTTAAGTC gacaatcaac ctctggatta caaaatttgt gaaagatt