Construct: ORF TRCN0000478243
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF005551.1_s317c1
- Derived from:
- ccsbBroadEn_11546
- DNA Barcode:
- TCTTTAAGGATATACCGATCACAC
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- AHCYL1 (10768)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000478243
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 10768 | AHCYL1 | adenosylhomocysteinase like 1 | NM_001242673.1 | 96.6% | 96.6% | 0_1ins51 |
| 2 | human | 10768 | AHCYL1 | adenosylhomocysteinase like 1 | NM_001242674.1 | 96.6% | 96.6% | 0_1ins51 |
| 3 | human | 10768 | AHCYL1 | adenosylhomocysteinase like 1 | NM_001242675.2 | 96.6% | 96.6% | 0_1ins51 |
| 4 | human | 10768 | AHCYL1 | adenosylhomocysteinase like 1 | NM_001242676.2 | 96.6% | 96.6% | 0_1ins51 |
| 5 | human | 10768 | AHCYL1 | adenosylhomocysteinase like 1 | NM_006621.7 | 94.3% | 94.3% | 1_90del |
| 6 | human | 10768 | AHCYL1 | adenosylhomocysteinase like 1 | XM_011540535.2 | 88.3% | 86.7% | (many diffs) |
| 7 | human | 23382 | AHCYL2 | adenosylhomocysteinase like 2 | NM_001130723.3 | 77.1% | 89.3% | (many diffs) |
| 8 | human | 23382 | AHCYL2 | adenosylhomocysteinase like 2 | NM_001130722.3 | 76.7% | 89.5% | (many diffs) |
| 9 | human | 23382 | AHCYL2 | adenosylhomocysteinase like 2 | NM_015328.4 | 64.9% | 75.4% | (many diffs) |
| 10 | human | 23382 | AHCYL2 | adenosylhomocysteinase like 2 | NM_001130720.3 | 64.8% | 75.2% | (many diffs) |
| 11 | human | 23382 | AHCYL2 | adenosylhomocysteinase like 2 | XM_017011905.2 | 63.8% | 75.6% | (many diffs) |
| 12 | human | 10768 | AHCYL1 | adenosylhomocysteinase like 1 | XR_001736934.2 | 39.5% | (many diffs) | |
| 13 | human | 10768 | AHCYL1 | adenosylhomocysteinase like 1 | XR_946527.2 | 39.1% | 1_92del;1318_1493del;1769_3833del | |
| 14 | human | 10768 | AHCYL1 | adenosylhomocysteinase like 1 | XR_001736935.1 | 34.7% | (many diffs) | |
| 15 | mouse | 229709 | Ahcyl1 | S-adenosylhomocysteine hydr... | XM_006501408.3 | 90.1% | 96.6% | (many diffs) |
| 16 | mouse | 229709 | Ahcyl1 | S-adenosylhomocysteine hydr... | NM_145542.3 | 88.1% | 94.3% | (many diffs) |
| 17 | mouse | 229709 | Ahcyl1 | S-adenosylhomocysteine hydr... | XM_017319556.1 | 58.2% | 62.6% | (many diffs) |
| 18 | mouse | 229709 | Ahcyl1 | S-adenosylhomocysteine hydr... | XM_017319557.1 | 58.2% | 62.6% | (many diffs) |
| 19 | mouse | 229709 | Ahcyl1 | S-adenosylhomocysteine hydr... | XM_017319558.1 | 53.2% | 54.4% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1566
- ORF length:
- 1500
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatggc caccgtcacc aaggcgccca agaagcaaat ccagtttgct gatgacatgc 121 aggagttcac caaattcccc accaaaactg gccgaagatc tttgtctcgc tcgatctcac 181 agtcctccac tgacagctac agttcagctg catcctacac agatagctct gatgatgagg 241 tttctccccg agagaagcag caaaccaact ccaagggcag cagcaatttc tgtgtgaaga 301 acatcaagca ggcagaattt ggacgccggg agattgagat tgcagagcaa gacatgtctg 361 ctctgatttc actcaggaaa cgtgctcagg gggagaagcc cttggctggt gctaaaatag 421 tgggctgtac acacatcaca gcccagacag cggtgttgat tgagacactc tgtgccctgg 481 gggctcagtg ccgctggtct gcttgtaaca tctactcaac tcagaatgaa gtagctgcag 541 cactggctga ggctggagtt gcagtgttcg cttggaaggg cgagtcagaa gatgacttct 601 ggtggtgtat tgaccgctgt gtgaacatgg atgggtggca ggccaacatg atcctggatg 661 atgggggaga cttaacccac tgggtttata agaagtatcc aaacgtgttt aagaagatcc 721 gaggcattgt ggaagagagc gtgactggtg ttcacaggct gtatcagctc tccaaagctg 781 ggaagctctg tgttccggcc atgaacgtca atgattctgt taccaaacag aagtttgata 841 acttgtactg ctgccgagaa tccattttgg atggcctgaa gaggaccaca gatgtgatgt 901 ttggtgggaa acaagtggtg gtgtgtggct atggtgaggt aggcaagggc tgctgtgctg 961 ctctcaaagc tcttggagca attgtctaca ttaccgaaat cgaccccatc tgtgctctgc 1021 aggcctgcat ggatgggttc agggtggtaa agctaaatga agtcatccgg caagtcgatg 1081 tcgtaataac ttgcacagga aataagaatg tagtgacacg ggagcacttg gatcgcatga 1141 aaaacagttg tatcgtatgc aatatgggcc actccaacac agaaatcgat gtgaccagcc 1201 tccgcactcc ggagctgacg tgggagcgag tacgttctca ggtggaccat gtcatctggc 1261 cagatggcaa acgagttgtc ctcctggcag agggtcgtct actcaattTG AGCTGCTCCA 1321 CAGTTCCCAC CTTTGTTCTG TCCATCACAG CCACAACACA GGCTTTGGCA CTGATAGAAC 1381 TCTATAATGC ACCCGAGGGG CGATACAAGC AGGATGTGTA CTTGCTTCCT AAGAAAATGG 1441 ATGAATACGT TGCCAGCTTG CATCTGCCAT CATTTGATGC CCACCTTACA GAGCTGACAG 1501 ATGACCAAGC AAAATATCTG GGACTCAACA AAAATGGGCC ATTCAAACCT AATTATTACA 1561 GATACTACCC AACTTTCTTG TACAAAGTGG TTGATATCGG TAAGCCTATC CCTAACCCTC 1621 TCCTCGGTCT CGATTCTACG TAGTAATGAA CTAGTCCGTA ACTTGAAAGT ATTTCGATTT 1681 CTTGGCTTTA TATATCTTGT GGAAAGGACG ATCTTTAAGG ATATACCGAT CACACACGCG 1741 TTAAGTCgac aatcaacctc tggattacaa aatttgtgaa agatt