Construct: ORF TRCN0000478243

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF005551.1_s317c1
Derived from:
ccsbBroadEn_11546
DNA Barcode:
TCTTTAAGGATATACCGATCACAC
Epitope Tag:
V5
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
AHCYL1 (10768)

Vector Information:

Vector Backbone:
pLX_317
Pol II Cassette 1:
SV40-PuroR
Pol II Cassette 2:
EF1a-TRCN0000478243
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
V5

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 10768 AHCYL1 adenosylhomocysteinase like 1 NM_001242673.1 96.6% 96.6% 0_1ins51
2 human 10768 AHCYL1 adenosylhomocysteinase like 1 NM_001242674.1 96.6% 96.6% 0_1ins51
3 human 10768 AHCYL1 adenosylhomocysteinase like 1 NM_001242675.2 96.6% 96.6% 0_1ins51
4 human 10768 AHCYL1 adenosylhomocysteinase like 1 NM_001242676.2 96.6% 96.6% 0_1ins51
5 human 10768 AHCYL1 adenosylhomocysteinase like 1 NM_006621.7 94.3% 94.3% 1_90del
6 human 10768 AHCYL1 adenosylhomocysteinase like 1 XM_011540535.2 88.3% 86.7% (many diffs)
7 human 23382 AHCYL2 adenosylhomocysteinase like 2 NM_001130723.3 77.1% 89.3% (many diffs)
8 human 23382 AHCYL2 adenosylhomocysteinase like 2 NM_001130722.3 76.7% 89.5% (many diffs)
9 human 23382 AHCYL2 adenosylhomocysteinase like 2 NM_015328.4 64.9% 75.4% (many diffs)
10 human 23382 AHCYL2 adenosylhomocysteinase like 2 NM_001130720.3 64.8% 75.2% (many diffs)
11 human 23382 AHCYL2 adenosylhomocysteinase like 2 XM_017011905.2 63.8% 75.6% (many diffs)
12 human 10768 AHCYL1 adenosylhomocysteinase like 1 XR_001736934.2 39.5% (many diffs)
13 human 10768 AHCYL1 adenosylhomocysteinase like 1 XR_946527.2 39.1% 1_92del;1318_1493del;1769_3833del
14 human 10768 AHCYL1 adenosylhomocysteinase like 1 XR_001736935.1 34.7% (many diffs)
15 mouse 229709 Ahcyl1 S-adenosylhomocysteine hydr... XM_006501408.3 90.1% 96.6% (many diffs)
16 mouse 229709 Ahcyl1 S-adenosylhomocysteine hydr... NM_145542.3 88.1% 94.3% (many diffs)
17 mouse 229709 Ahcyl1 S-adenosylhomocysteine hydr... XM_017319556.1 58.2% 62.6% (many diffs)
18 mouse 229709 Ahcyl1 S-adenosylhomocysteine hydr... XM_017319557.1 58.2% 62.6% (many diffs)
19 mouse 229709 Ahcyl1 S-adenosylhomocysteine hydr... XM_017319558.1 53.2% 54.4% (many diffs)
Download CSV

Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
66
ORF end:
1566
ORF length:
1500
Sequence:
1tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag
61ttggcatggc caccgtcacc aaggcgccca agaagcaaat ccagtttgct gatgacatgc
121aggagttcac caaattcccc accaaaactg gccgaagatc tttgtctcgc tcgatctcac
181agtcctccac tgacagctac agttcagctg catcctacac agatagctct gatgatgagg
241tttctccccg agagaagcag caaaccaact ccaagggcag cagcaatttc tgtgtgaaga
301acatcaagca ggcagaattt ggacgccggg agattgagat tgcagagcaa gacatgtctg
361ctctgatttc actcaggaaa cgtgctcagg gggagaagcc cttggctggt gctaaaatag
421tgggctgtac acacatcaca gcccagacag cggtgttgat tgagacactc tgtgccctgg
481gggctcagtg ccgctggtct gcttgtaaca tctactcaac tcagaatgaa gtagctgcag
541cactggctga ggctggagtt gcagtgttcg cttggaaggg cgagtcagaa gatgacttct
601ggtggtgtat tgaccgctgt gtgaacatgg atgggtggca ggccaacatg atcctggatg
661atgggggaga cttaacccac tgggtttata agaagtatcc aaacgtgttt aagaagatcc
721gaggcattgt ggaagagagc gtgactggtg ttcacaggct gtatcagctc tccaaagctg
781ggaagctctg tgttccggcc atgaacgtca atgattctgt taccaaacag aagtttgata
841acttgtactg ctgccgagaa tccattttgg atggcctgaa gaggaccaca gatgtgatgt
901ttggtgggaa acaagtggtg gtgtgtggct atggtgaggt aggcaagggc tgctgtgctg
961ctctcaaagc tcttggagca attgtctaca ttaccgaaat cgaccccatc tgtgctctgc
1021aggcctgcat ggatgggttc agggtggtaa agctaaatga agtcatccgg caagtcgatg
1081tcgtaataac ttgcacagga aataagaatg tagtgacacg ggagcacttg gatcgcatga
1141aaaacagttg tatcgtatgc aatatgggcc actccaacac agaaatcgat gtgaccagcc
1201tccgcactcc ggagctgacg tgggagcgag tacgttctca ggtggaccat gtcatctggc
1261cagatggcaa acgagttgtc ctcctggcag agggtcgtct actcaattTG AGCTGCTCCA
1321CAGTTCCCAC CTTTGTTCTG TCCATCACAG CCACAACACA GGCTTTGGCA CTGATAGAAC
1381TCTATAATGC ACCCGAGGGG CGATACAAGC AGGATGTGTA CTTGCTTCCT AAGAAAATGG
1441ATGAATACGT TGCCAGCTTG CATCTGCCAT CATTTGATGC CCACCTTACA GAGCTGACAG
1501ATGACCAAGC AAAATATCTG GGACTCAACA AAAATGGGCC ATTCAAACCT AATTATTACA
1561GATACTACCC AACTTTCTTG TACAAAGTGG TTGATATCGG TAAGCCTATC CCTAACCCTC
1621TCCTCGGTCT CGATTCTACG TAGTAATGAA CTAGTCCGTA ACTTGAAAGT ATTTCGATTT
1681CTTGGCTTTA TATATCTTGT GGAAAGGACG ATCTTTAAGG ATATACCGAT CACACACGCG
1741TTAAGTCgac aatcaacctc tggattacaa aatttgtgaa agatt

Download FASTA (ORF) (Full)