Construct: ORF TRCN0000478285
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF016618.1_s317c1
- Derived from:
- ccsbBroadEn_00443
- DNA Barcode:
- GCAGTACTGAGTGTACAAGCGTAG
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- DHFR (1719)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000478285
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 1719 | DHFR | dihydrofolate reductase | NM_000791.4 | 100% | 100% | |
| 2 | human | 200895 | DHFR2 | dihydrofolate reductase 2 | NM_001195643.2 | 94.8% | 91.9% | (many diffs) |
| 3 | human | 200895 | DHFR2 | dihydrofolate reductase 2 | NM_176815.5 | 94.8% | 91.9% | (many diffs) |
| 4 | human | 200895 | DHFR2 | dihydrofolate reductase 2 | XM_011512537.3 | 94.8% | 91.9% | (many diffs) |
| 5 | human | 1719 | DHFR | dihydrofolate reductase | NM_001290354.2 | 72.1% | 72.1% | 0_1ins156 |
| 6 | human | 1719 | DHFR | dihydrofolate reductase | NM_001290357.2 | 68.9% | 64.7% | 369_370ins116;387_388ins58 |
| 7 | human | 1720 | DHFRP3 | dihydrofolate reductase pse... | NR_033423.1 | 53% | (many diffs) | |
| 8 | human | 1719 | DHFR | dihydrofolate reductase | NR_110936.2 | 9.7% | (many diffs) | |
| 9 | mouse | 13361 | Dhfr | dihydrofolate reductase | NM_010049.3 | 88.4% | 89.8% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 627
- ORF length:
- 561
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatggt tggttcgcta aactgcatcg tcgctgtgtc ccagaacatg ggcatcggca 121 agaacgggga cctgccctgg ccaccgctca ggaatgaatt cagatatttc cagagaatga 181 ccacaacctc ttcagtagaa ggtaaacaga atctggtgat tatgggtaag aagacctggt 241 tctccattcc tgagaagaat cgacctttaa agggtagaat taatttagtt ctcagcagag 301 aactcaagga accTCCACAA GGAGCTCATT TTCTTTCCAG AAGTCTAGAT GATGCCTTAA 361 AACTTACTGA ACAACCAGAA TTAGCAAATA AAGTAGACAT GGTCTGGATA GTTGGTGGCA 421 GTTCTGTTTA TAAGGAAGCC ATGAATCACC CAGGCCATCT TAAACTATTT GTGACAAGGA 481 TCATGCAAGA CTTTGAAAGT GACACGTTTT TTCCAGAAAT TGATTTGGAG AAATATAAAC 541 TTCTGCCAGA ATACCCAGGT GTTCTCTCTG ATGTCCAGGA GGAGAAAGGC ATTAAGTACA 601 AATTTGAAGT ATATGAGAAG AATGATTACC CAACTTTCTT GTACAAAGTG GTTGATATCG 661 GTAAGCCTAT CCCTAACCCT CTCCTCGGTC TCGATTCTAC GTAGTAATGA ACTAGTCCGT 721 AACTGAAAGT ATTTCGATTT CTTGGCTTTA TATATCTTGT GGAAAGGACG AGCAGTACTG 781 AGTGTACAAG CGTAGACGCG TTAAGTCgac aatcaacctc tggattacaa aatttgtgaa 841 agatt