Construct: ORF TRCN0000478305

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF004976.1_s317c1
Derived from:
ccsbBroadEn_07384
DNA Barcode:
CGCGCGAGCACGAGATCGCAAACA
Epitope Tag:
V5
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
PIWIL1 (9271)

Vector Information:

Vector Backbone:
pLX_317
Pol II Cassette 1:
SV40-PuroR
Pol II Cassette 2:
EF1a-TRCN0000478305
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
n/a

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 9271 PIWIL1 piwi like RNA-mediated gene... NM_004764.5 99.8% 99.6% 1473G>T;1580G>A;1724T>C
2 human 9271 PIWIL1 piwi like RNA-mediated gene... XM_011539002.3 99.8% 99.6% 1473G>T;1580G>A;1724T>C
3 human 9271 PIWIL1 piwi like RNA-mediated gene... XM_011539003.3 99.8% 99.6% 1473G>T;1580G>A;1724T>C
4 human 9271 PIWIL1 piwi like RNA-mediated gene... XM_011539004.3 99.8% 99.6% 1473G>T;1580G>A;1724T>C
5 human 9271 PIWIL1 piwi like RNA-mediated gene... XM_024449277.1 99.8% 99.6% 1473G>T;1580G>A;1724T>C
6 human 9271 PIWIL1 piwi like RNA-mediated gene... XM_017020229.1 96.3% 95.3% (many diffs)
7 human 9271 PIWIL1 piwi like RNA-mediated gene... NM_001190971.2 95.8% 95.4% (many diffs)
8 human 9271 PIWIL1 piwi like RNA-mediated gene... XM_011539005.1 95% 94.6% (many diffs)
9 human 9271 PIWIL1 piwi like RNA-mediated gene... XM_011539006.3 55.7% 55.5% (many diffs)
10 mouse 57749 Piwil1 piwi-like RNA-mediated gene... NM_021311.3 82% 94.7% (many diffs)
11 mouse 57749 Piwil1 piwi-like RNA-mediated gene... XM_006504318.1 82% 94.7% (many diffs)
Download CSV

Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
66
ORF end:
2649
ORF length:
2583
Sequence:
1tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag
61ttggcatgac tgggagagcc cgagccagag ccagaggaag ggcccgcggt caggagacag
121cgcagctggt gggctccact gccagtcagc aacctggtta tattcagcct aggcctcagc
181cgccaccagc agagggggaa ttatttggcc gtggacggca gagaggaaca gcaggaggaa
241cagccaagtc acaaggactc cagatatctg ctggatttca ggagttatcg ttagcagaga
301gaggaggtcg tcgtagagat tttcatgatc ttggtgtgaa tacaaggcag aacctagacc
361atgttaaaga atcaaaaaca ggttcttcag gcattatagt aaggttaagc actaaccatt
421tccggctgac atcccgtccc cagtgggcct tatatcagta tcacattgac tataacccac
481tgatggaagc cagaagactc cgttcagctc ttctttttca acacgaagat ctaattggaa
541agtgtcatgc ttttgatgga acgatattat ttttacctaa aagactacag caaaaggtta
601ctgaagtttt tagtaagacc cggaatggag aggatgtgag gataacgatc actttaacaa
661atgaacttcc acctacatca ccaacttgtt tgcagttcta taatattatt ttcaggaggc
721ttttgaaaat catgaatttg caacaaattg gacgaaatta ttataaccca aatgacccaa
781ttgatattcc aagtcacagg ttggtgattt ggcctggctt cactacttcc atccttcagt
841atgaaaacag catcatgctc tgcactgacg ttagccataa agtccttcga agtgagactg
901ttttggattt catgttcaac ttttatcatc agacagaaga acataaattt caagaacaag
961tttccaaaga actaataggt ttagttgttc ttaccaagta taacaataag acatacagag
1021tggatgatat tgactgggac cagaatccca agagcacctt taagaaagcc gacggctctg
1081aagtcagctt cttagaatac tacaggaagc aatacaacca agagatcacc gacttgaagc
1141agcctgtctt ggtcagccag cccaagagaa ggcggggccc tggggggaca ctgccagggc
1201ctgccatgct cattcctgag ctctgctatc ttacaggtct aactgataaa atgcgtaatg
1261attttaacgt gatgaaagac ttagccgttc atacaagact aactccagag caaaggcagc
1321gtgaagtggg acgactcatt gattacattc ataaaaacga taatgttcaa agggagcttc
1381gagactgggg tttgagcttt gattccaact tactgtcctt ctcaggaaga attttgcaaa
1441cagaaaagat tcaccaaggt ggaaaaacat ttgattacaa tccacaattt gcagattggt
1501ccaaagaaac aagaggtgca ccattaatta gtgttaatcc actagataac tggctgttga
1561tctatacgcg aagaaattat gaagcagcca attcattgat acaaaatcta tttaaagtta
1621caccagccat gggcatgcaa atgaaaaaag caataatgat tgaagtggat gacagaactg
1681aagcctactt aagagtctta cagcaaaagg tcacagcaga cacccagata gttgtctgtc
1741tgttgtcaag taatcggaag gacaaatacg atgctattaa aaaatacccg tgtacagatt
1801gccctacccc aagtcagtgt gtggtggccc gaaccttagg caaacagcaa actgtcatgg
1861ccattgctac aaagattgcc ctacagatga actgcaagat gggaggagag ctctggaggg
1921tggacatccc cctgaagctc gtgatgatcg ttggcatcga ttgttaccat gacatgacag
1981ctgggcggag gtcaatcgca ggatttgttg ccagcatcaa tgaagggatg acccgctggt
2041tctcacgctg catatttcag gatagaggac aggagctggt agatgggctc aaagtctgcc
2101tgcaagcggc tctgagggct tggaatagct gcaatgagta catgcccagc cggatcatcg
2161tgtaccgcga tggcgtagga gacggccagc tgaaaacact ggtgaactac gaagtgccac
2221agtttttgga ttgtctaaaa tccattggta gaggttacaa ccctagacta acggtaattg
2281tggtgaagaa aagagtgaac accagatttt ttgctcagtc tggaggaaga cttcagaaTC
2341CACTTCCTGG AACAGTTATT GATGTAGAGG TTACCAGACC AGAATGGTAT GACTTTTTTA
2401TCGTGAGCCA GGCTGTGAGA AGTGGTAGTG TTTCTCCCAC ACATTACAAT GTCATCTATG
2461ACAACAGCGG CCTGAAGCCA GACCACATAC AGCGCTTGAC CTACAAGCTG TGCCACATCT
2521ATTACAACTG GCCAGGTGTC ATTCGTGTTC CTGCTCCTTG CCAGTACGCC CACAAGCTGG
2581CTTTTCTTGT TGGCCAGAGT ATTCACAGAG AGCCAAATCT GTCACTGTCA AACCGCCTTT
2641ACTACCTCTA CCCAACTTTC TTGTACAAAG TGGTTGATAT CGGTAAGCCT ATCCCTAACC
2701CTCTCCTCGG TCTCGATTCT ACGTAGTAAT GAACTAGTCC GTAACTTGAA AGTATTTCGA
2761TTTCTTGGCT TTATATATCT TGTGGAAAGG ACGACGCGCG AGCACGAGAT CGCAAACAAC
2821GCGTTAAGTC gacaatcaac ctctggatta caaaatttgt gaaagatt

Download FASTA (ORF) (Full)

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