Construct: ORF TRCN0000478305
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF004976.1_s317c1
- Derived from:
- ccsbBroadEn_07384
- DNA Barcode:
- CGCGCGAGCACGAGATCGCAAACA
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- PIWIL1 (9271)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000478305
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 9271 | PIWIL1 | piwi like RNA-mediated gene... | NM_004764.5 | 99.8% | 99.6% | 1473G>T;1580G>A;1724T>C |
| 2 | human | 9271 | PIWIL1 | piwi like RNA-mediated gene... | XM_011539002.3 | 99.8% | 99.6% | 1473G>T;1580G>A;1724T>C |
| 3 | human | 9271 | PIWIL1 | piwi like RNA-mediated gene... | XM_011539003.3 | 99.8% | 99.6% | 1473G>T;1580G>A;1724T>C |
| 4 | human | 9271 | PIWIL1 | piwi like RNA-mediated gene... | XM_011539004.3 | 99.8% | 99.6% | 1473G>T;1580G>A;1724T>C |
| 5 | human | 9271 | PIWIL1 | piwi like RNA-mediated gene... | XM_024449277.1 | 99.8% | 99.6% | 1473G>T;1580G>A;1724T>C |
| 6 | human | 9271 | PIWIL1 | piwi like RNA-mediated gene... | XM_017020229.1 | 96.3% | 95.3% | (many diffs) |
| 7 | human | 9271 | PIWIL1 | piwi like RNA-mediated gene... | NM_001190971.2 | 95.8% | 95.4% | (many diffs) |
| 8 | human | 9271 | PIWIL1 | piwi like RNA-mediated gene... | XM_011539005.1 | 95% | 94.6% | (many diffs) |
| 9 | human | 9271 | PIWIL1 | piwi like RNA-mediated gene... | XM_011539006.3 | 55.7% | 55.5% | (many diffs) |
| 10 | mouse | 57749 | Piwil1 | piwi-like RNA-mediated gene... | NM_021311.3 | 82% | 94.7% | (many diffs) |
| 11 | mouse | 57749 | Piwil1 | piwi-like RNA-mediated gene... | XM_006504318.1 | 82% | 94.7% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 2649
- ORF length:
- 2583
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgac tgggagagcc cgagccagag ccagaggaag ggcccgcggt caggagacag 121 cgcagctggt gggctccact gccagtcagc aacctggtta tattcagcct aggcctcagc 181 cgccaccagc agagggggaa ttatttggcc gtggacggca gagaggaaca gcaggaggaa 241 cagccaagtc acaaggactc cagatatctg ctggatttca ggagttatcg ttagcagaga 301 gaggaggtcg tcgtagagat tttcatgatc ttggtgtgaa tacaaggcag aacctagacc 361 atgttaaaga atcaaaaaca ggttcttcag gcattatagt aaggttaagc actaaccatt 421 tccggctgac atcccgtccc cagtgggcct tatatcagta tcacattgac tataacccac 481 tgatggaagc cagaagactc cgttcagctc ttctttttca acacgaagat ctaattggaa 541 agtgtcatgc ttttgatgga acgatattat ttttacctaa aagactacag caaaaggtta 601 ctgaagtttt tagtaagacc cggaatggag aggatgtgag gataacgatc actttaacaa 661 atgaacttcc acctacatca ccaacttgtt tgcagttcta taatattatt ttcaggaggc 721 ttttgaaaat catgaatttg caacaaattg gacgaaatta ttataaccca aatgacccaa 781 ttgatattcc aagtcacagg ttggtgattt ggcctggctt cactacttcc atccttcagt 841 atgaaaacag catcatgctc tgcactgacg ttagccataa agtccttcga agtgagactg 901 ttttggattt catgttcaac ttttatcatc agacagaaga acataaattt caagaacaag 961 tttccaaaga actaataggt ttagttgttc ttaccaagta taacaataag acatacagag 1021 tggatgatat tgactgggac cagaatccca agagcacctt taagaaagcc gacggctctg 1081 aagtcagctt cttagaatac tacaggaagc aatacaacca agagatcacc gacttgaagc 1141 agcctgtctt ggtcagccag cccaagagaa ggcggggccc tggggggaca ctgccagggc 1201 ctgccatgct cattcctgag ctctgctatc ttacaggtct aactgataaa atgcgtaatg 1261 attttaacgt gatgaaagac ttagccgttc atacaagact aactccagag caaaggcagc 1321 gtgaagtggg acgactcatt gattacattc ataaaaacga taatgttcaa agggagcttc 1381 gagactgggg tttgagcttt gattccaact tactgtcctt ctcaggaaga attttgcaaa 1441 cagaaaagat tcaccaaggt ggaaaaacat ttgattacaa tccacaattt gcagattggt 1501 ccaaagaaac aagaggtgca ccattaatta gtgttaatcc actagataac tggctgttga 1561 tctatacgcg aagaaattat gaagcagcca attcattgat acaaaatcta tttaaagtta 1621 caccagccat gggcatgcaa atgaaaaaag caataatgat tgaagtggat gacagaactg 1681 aagcctactt aagagtctta cagcaaaagg tcacagcaga cacccagata gttgtctgtc 1741 tgttgtcaag taatcggaag gacaaatacg atgctattaa aaaatacccg tgtacagatt 1801 gccctacccc aagtcagtgt gtggtggccc gaaccttagg caaacagcaa actgtcatgg 1861 ccattgctac aaagattgcc ctacagatga actgcaagat gggaggagag ctctggaggg 1921 tggacatccc cctgaagctc gtgatgatcg ttggcatcga ttgttaccat gacatgacag 1981 ctgggcggag gtcaatcgca ggatttgttg ccagcatcaa tgaagggatg acccgctggt 2041 tctcacgctg catatttcag gatagaggac aggagctggt agatgggctc aaagtctgcc 2101 tgcaagcggc tctgagggct tggaatagct gcaatgagta catgcccagc cggatcatcg 2161 tgtaccgcga tggcgtagga gacggccagc tgaaaacact ggtgaactac gaagtgccac 2221 agtttttgga ttgtctaaaa tccattggta gaggttacaa ccctagacta acggtaattg 2281 tggtgaagaa aagagtgaac accagatttt ttgctcagtc tggaggaaga cttcagaaTC 2341 CACTTCCTGG AACAGTTATT GATGTAGAGG TTACCAGACC AGAATGGTAT GACTTTTTTA 2401 TCGTGAGCCA GGCTGTGAGA AGTGGTAGTG TTTCTCCCAC ACATTACAAT GTCATCTATG 2461 ACAACAGCGG CCTGAAGCCA GACCACATAC AGCGCTTGAC CTACAAGCTG TGCCACATCT 2521 ATTACAACTG GCCAGGTGTC ATTCGTGTTC CTGCTCCTTG CCAGTACGCC CACAAGCTGG 2581 CTTTTCTTGT TGGCCAGAGT ATTCACAGAG AGCCAAATCT GTCACTGTCA AACCGCCTTT 2641 ACTACCTCTA CCCAACTTTC TTGTACAAAG TGGTTGATAT CGGTAAGCCT ATCCCTAACC 2701 CTCTCCTCGG TCTCGATTCT ACGTAGTAAT GAACTAGTCC GTAACTTGAA AGTATTTCGA 2761 TTTCTTGGCT TTATATATCT TGTGGAAAGG ACGACGCGCG AGCACGAGAT CGCAAACAAC 2821 GCGTTAAGTC gacaatcaac ctctggatta caaaatttgt gaaagatt