Construct: ORF TRCN0000478641

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF000884.1_s317c1
Derived from:
ccsbBroadEn_06559
DNA Barcode:
CGCTTCTGCGATGCTCAGGACAGG
Epitope Tag:
V5
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
MAP2 (4133)

Vector Information:

Vector Backbone:
pLX_317
Pol II Cassette 1:
SV40-PuroR
Pol II Cassette 2:
EF1a-TRCN0000478641
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
V5

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 4133 MAP2 microtubule associated prot... NM_001039538.1 99.9% 100% 21T>C
2 human 4133 MAP2 microtubule associated prot... XM_017004138.2 99.9% 100% 21T>C
3 human 4133 MAP2 microtubule associated prot... NM_031847.2 89.7% 89.8% 21T>C;516_517ins171
4 human 4133 MAP2 microtubule associated prot... XM_024452910.1 89.7% 89.8% 21T>C;516_517ins171
5 human 4133 MAP2 microtubule associated prot... NM_001363913.1 84.1% 84.2% 21T>C;516_517ins171;1005_1006ins93
6 human 4133 MAP2 microtubule associated prot... NM_031845.2 84.1% 84.2% 21T>C;516_517ins171;1005_1006ins93
7 human 4133 MAP2 microtubule associated prot... XM_024452911.1 84.1% 84.2% 21T>C;516_517ins171;1005_1006ins93
8 human 4133 MAP2 microtubule associated prot... XM_017004116.2 29.8% 27.9% (many diffs)
9 human 4133 MAP2 microtubule associated prot... XM_024452897.1 29.8% 27.8% (many diffs)
10 human 4133 MAP2 microtubule associated prot... XM_024452899.1 29.8% 27.8% (many diffs)
11 human 4133 MAP2 microtubule associated prot... XM_017004114.2 29.7% 27.7% (many diffs)
12 human 4133 MAP2 microtubule associated prot... XM_024452896.1 29.7% 27.7% (many diffs)
13 human 4133 MAP2 microtubule associated prot... XM_005246565.1 29.5% 25.3% (many diffs)
14 human 4133 MAP2 microtubule associated prot... XM_005246566.1 29.5% 25.3% (many diffs)
15 human 4133 MAP2 microtubule associated prot... XM_017004130.1 29.5% 25.3% (many diffs)
16 human 4133 MAP2 microtubule associated prot... XM_017004131.1 28.8% 22.1% (many diffs)
17 human 4133 MAP2 microtubule associated prot... XM_017004132.1 28.8% 22.1% (many diffs)
18 human 4133 MAP2 microtubule associated prot... XM_017004125.2 28.2% 26.2% (many diffs)
19 human 4133 MAP2 microtubule associated prot... XM_017004126.2 28.2% 26.2% (many diffs)
20 human 4133 MAP2 microtubule associated prot... XM_024452905.1 28.2% 26.2% (many diffs)
21 human 4133 MAP2 microtubule associated prot... XM_024452906.1 28.2% 26.2% (many diffs)
22 human 4133 MAP2 microtubule associated prot... NM_001363910.1 28.1% 26.2% (many diffs)
23 human 4133 MAP2 microtubule associated prot... NM_001363911.1 28.1% 26.2% (many diffs)
24 human 4133 MAP2 microtubule associated prot... XM_017004124.2 28.1% 26.2% (many diffs)
25 human 4133 MAP2 microtubule associated prot... XM_017004129.2 28.1% 26.1% (many diffs)
26 human 4133 MAP2 microtubule associated prot... XM_017004128.2 28.1% 26.1% (many diffs)
27 human 4133 MAP2 microtubule associated prot... XM_017004119.2 28.1% 26.1% (many diffs)
28 human 4133 MAP2 microtubule associated prot... XM_017004121.2 28.1% 26.1% (many diffs)
29 human 4133 MAP2 microtubule associated prot... XM_024452901.1 28.1% 26.1% (many diffs)
30 human 4133 MAP2 microtubule associated prot... XM_017004127.2 28.1% 26.1% (many diffs)
31 human 4133 MAP2 microtubule associated prot... XM_024452907.1 28.1% 26.1% (many diffs)
32 human 4133 MAP2 microtubule associated prot... NM_002374.3 28.1% 26.1% (many diffs)
33 human 4133 MAP2 microtubule associated prot... XM_017004118.2 28.1% 26.1% (many diffs)
34 human 4133 MAP2 microtubule associated prot... XM_024452900.1 28.1% 26.1% (many diffs)
35 human 4133 MAP2 microtubule associated prot... XM_017004120.2 28% 26% (many diffs)
36 human 4133 MAP2 microtubule associated prot... XM_017004122.2 28% 26% (many diffs)
37 human 4133 MAP2 microtubule associated prot... XM_024452902.1 28% 26% (many diffs)
38 human 4133 MAP2 microtubule associated prot... XM_017004137.1 27.1% 20.3% (many diffs)
39 human 4133 MAP2 microtubule associated prot... XM_011511197.3 27.1% 29.1% (many diffs)
40 human 4133 MAP2 microtubule associated prot... XM_017004113.2 27.1% 29.1% (many diffs)
41 human 4133 MAP2 microtubule associated prot... XM_024452895.1 27.1% 29.1% (many diffs)
42 human 4133 MAP2 microtubule associated prot... XM_011511196.3 27.1% 29.2% (many diffs)
43 human 4133 MAP2 microtubule associated prot... XM_024452894.1 27.1% 29.2% (many diffs)
44 human 4133 MAP2 microtubule associated prot... XM_011511195.2 27.1% 29.1% (many diffs)
45 human 4133 MAP2 microtubule associated prot... XM_017004112.2 27% 29.1% (many diffs)
46 human 4133 MAP2 microtubule associated prot... XM_024452891.1 27% 29.1% (many diffs)
47 human 4133 MAP2 microtubule associated prot... XM_024452893.1 27% 29.1% (many diffs)
48 human 4133 MAP2 microtubule associated prot... XM_017004136.1 27% 20.3% (many diffs)
49 human 4133 MAP2 microtubule associated prot... XM_017004133.1 27% 20.3% (many diffs)
50 human 4133 MAP2 microtubule associated prot... XM_017004134.1 27% 20.3% (many diffs)
51 mouse 17756 Map2 microtubule-associated prot... NM_001039934.1 79.3% 84.6% (many diffs)
52 mouse 17756 Map2 microtubule-associated prot... XM_006495759.4 79.3% 84.6% (many diffs)
53 mouse 17756 Map2 microtubule-associated prot... XM_006495760.2 79.3% 84.6% (many diffs)
54 mouse 17756 Map2 microtubule-associated prot... XM_030251982.1 79.3% 84.6% (many diffs)
55 mouse 17756 Map2 microtubule-associated prot... XM_030251986.1 79.3% 84.6% (many diffs)
56 mouse 17756 Map2 microtubule-associated prot... XM_006495761.4 79.1% 84.4% (many diffs)
57 mouse 17756 Map2 microtubule-associated prot... XM_030251989.1 79.1% 84.4% (many diffs)
58 mouse 17756 Map2 microtubule-associated prot... XM_030251990.1 79.1% 84.4% (many diffs)
59 mouse 17756 Map2 microtubule-associated prot... XM_030251993.1 79.1% 84.4% (many diffs)
60 mouse 17756 Map2 microtubule-associated prot... NM_008632.2 73.9% 79% (many diffs)
61 mouse 17756 Map2 microtubule-associated prot... XM_006495762.4 73.9% 79% (many diffs)
62 mouse 17756 Map2 microtubule-associated prot... XM_017319194.2 73.9% 79% (many diffs)
63 mouse 17756 Map2 microtubule-associated prot... XM_030251996.1 73.9% 79% (many diffs)
64 mouse 17756 Map2 microtubule-associated prot... XM_006495753.3 24.2% 26.7% (many diffs)
65 mouse 17756 Map2 microtubule-associated prot... XM_030251971.1 24.2% 26.7% (many diffs)
66 mouse 17756 Map2 microtubule-associated prot... XM_006495751.3 24.1% 26.6% (many diffs)
67 mouse 17756 Map2 microtubule-associated prot... XM_017319165.2 24.1% 26.6% (many diffs)
68 mouse 17756 Map2 microtubule-associated prot... XM_006495746.3 24.1% 26.8% (many diffs)
69 mouse 17756 Map2 microtubule-associated prot... XM_006495747.4 24.1% 26.8% (many diffs)
70 mouse 17756 Map2 microtubule-associated prot... XM_006495748.4 24.1% 26.8% (many diffs)
71 mouse 17756 Map2 microtubule-associated prot... XM_006495749.4 24.1% 26.8% (many diffs)
72 mouse 17756 Map2 microtubule-associated prot... XM_011238448.2 24.1% 26.8% (many diffs)
73 mouse 17756 Map2 microtubule-associated prot... XM_006495752.3 24% 26.7% (many diffs)
74 mouse 17756 Map2 microtubule-associated prot... XM_006495750.3 24% 26.8% (many diffs)
Download CSV

Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
66
ORF end:
1743
ORF length:
1677
Sequence:
1tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag
61ttggcatggc agatgaacgg aaagacgaag caaaggcacc tcactggacc tcagcaccgc
121taacagaggc atctgcacac tcacatccac ctgagattaa ggatcaaggc ggagcagggg
181aaggacttgt ccgaagcgcc aatggattcc catacaggga ggatgaagag ggtgcctttg
241gagagcatgg gtcacagggc acctattcaa ataccaaaga gaatgggatc aacggagagc
301tgacctcagc tgacagagaa acagcagagg aggtgtctgc aaggatagtt caagtagtca
361ctgctgaggc tgtagcagtc ctgaaaggtg aacaagagaa agaagctcaa cataaagacc
421agactgcagc tctgccttta gcagctgaag aaacagctaa tctgcctcct tctccacccc
481catcacctgc ctcagaacag actgtcacag tggaggaagc agcaggtggg gaatcagctc
541tggctcccag tgtatttaaa caggcaaagg acaaagtctc taattctacc ttgtcaaaga
601ttcctgcttt acagggtagc acaaagtccc caagatacag ctcagcctgc cctagcacga
661ctaaaagggc tacattttct gacagtttat taatacagcc cacctcagca ggctccacag
721accgtttgcc atactcaaaa tcagggaaca aggacggagt aaccaagagc ccagaaaagc
781gctcttctct cccaagacct tcctccattc tccctcctcg gcgaggtgtg tcaggagaca
841gagatgagaa ttccttctct ctcaacagtt ctatctcttc ttcagcacgg cggaccacca
901ggtcagagcc aattcgcaga gcagggaaga gtggtacctc aacacccact acccctgggt
961ctactgccat cactcctggc accccaccaa gttattcttc acgcacacca ggcactcctg
1021gaacccctag ctatcccagg acccctcaca caccaggaac ccccaagtct gccatcttgg
1081tgccgagtga gaagaaggtc gccatcatac gtactcctcc aaaatctcct gcgactccca
1141agcagcttcg gcttattaac caaccactgc cagacctgaa gaatgtcaaa tccaaaatcg
1201gatcaacaga caacatcaaa taccagccta aaggggggca ggttaggatt ttaaacaaga
1261agatcgattt tagcaaagtt cagtccagat gtggttccaa ggataacatc aaacattcgg
1321ctgggggcgg aaatgtacaa attgttacca agaaaataga ccTAAGCCAT GTGACATCCA
1381AATGTGGCTC TCTGAAGAAC ATCCGCCACA GGCCAGGTGG CGGACGTGTG AAAATTGAGA
1441GTGTAAAACT AGATTTCAAA GAAAAGGCCC AAGCTAAAGT TGGTTCTCTT GATAATGCTC
1501ATCATGTACC TGGAGGTGGT AATGTCAAGA TTGACAGCCA AAAGTTGAAC TTCAGAGAGC
1561ATGCTAAAGC CCGTGTGGAC CATGGGGCTG AGATCATTAC ACAGTCCCCA GGCAGATCCA
1621GCGTGGCATC ACCCCGACGA CTCAGCAATG TCTCCTCGTC TGGAAGCATC AACCTGCTCG
1681AATCTCCTCA GCTTGCCACT TTGGCTGAGG ATGTCACTGC TGCACTCGCT AAGCAGGGCT
1741TGTGCCCAAC TTTCTTGTAC AAAGTGGTTG ATATCGGTAA GCCTATCCCT AACCCTCTCC
1801TCGGTCTCGA TTCTACGTAG TAATGAACTA GTCCGTAACT TGAAAGTATT TCGATTTCTT
1861GGCTTTATAT ATCTTGTGGA AAGGACGACG CTTCTGCGAT GCTCAGGACA GGACGCGTTA
1921AGTCgacaat caacctctgg attacaaaat ttgtgaaaga tt

Download FASTA (ORF) (Full)