Construct: ORF TRCN0000478777
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF010545.3_s317c1
- Derived from:
- ccsbBroadEn_14753
- DNA Barcode:
- TGTCCCTAGTTAATCGCTATTCCA
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- PHKB (5257)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000478777
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 5257 | PHKB | phosphorylase kinase regula... | NM_001031835.3 | 99.8% | 99.7% | (many diffs) |
| 2 | human | 5257 | PHKB | phosphorylase kinase regula... | NM_001363837.1 | 98% | 97.9% | (many diffs) |
| 3 | human | 5257 | PHKB | phosphorylase kinase regula... | NM_000293.3 | 96.7% | 96.5% | (many diffs) |
| 4 | mouse | 102093 | Phkb | phosphorylase kinase beta | NM_199446.2 | 88.2% | 92.8% | (many diffs) |
| 5 | mouse | 102093 | Phkb | phosphorylase kinase beta | XM_006530532.4 | 88.2% | 92.8% | (many diffs) |
| 6 | mouse | 102093 | Phkb | phosphorylase kinase beta | XM_006530533.4 | 88.2% | 92.8% | (many diffs) |
| 7 | mouse | 102093 | Phkb | phosphorylase kinase beta | XM_006530534.4 | 88.2% | 92.8% | (many diffs) |
| 8 | mouse | 102093 | Phkb | phosphorylase kinase beta | NM_001364412.1 | 86.9% | 91.6% | (many diffs) |
| 9 | mouse | 102093 | Phkb | phosphorylase kinase beta | XM_006530531.4 | 85.6% | 90.3% | (many diffs) |
| 10 | mouse | 102093 | Phkb | phosphorylase kinase beta | NM_001364411.1 | 83.8% | 88.5% | (many diffs) |
| 11 | mouse | 102093 | Phkb | phosphorylase kinase beta | XM_006530537.4 | 69.9% | 73.2% | (many diffs) |
| 12 | mouse | 102093 | Phkb | phosphorylase kinase beta | XR_878767.3 | 35.1% | (many diffs) | |
| 13 | mouse | 102093 | Phkb | phosphorylase kinase beta | XR_001778386.2 | 33.7% | (many diffs) | |
| 14 | mouse | 102093 | Phkb | phosphorylase kinase beta | XR_001778385.2 | 33.5% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 3324
- ORF length:
- 3258
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatggc ctgctcacct gatgcagtcg tctctccgtc ttccgctttc ttaaggtctg 121 gctcagttta tgaacctctt aaaagcatta atcttccaag acctgataat gaaactctct 181 gggataagtt ggaccattat tacagaattg tcaagtcaac attgctgctg tatcaaagtc 241 caactaccgg tctctttccc actaaaacat gcggtggtga ccagaaggcc aagatccagg 301 acagcctata ctgcgctgct ggggcctggg ctttggctct tgcatacagg cgaattgatg 361 acgacaaggg aaggacccat gagctggagc actcagctat aaaatgcatg agaggaattc 421 tctactgcta tatgcgtcag gccgataagg tccagcagtt taagcaggat ccacgcccaa 481 caacatgtct tcactctgtt ttcaatgtgc atacaggaga tgagttgctt tcctatgagg 541 aatatggtca tcttcagata aatgcagtgt cactttatct cctttacctt gtggaaatga 601 tttcctcagg actccagatt atctacaaca ctgatgaggt ctcttttatt caaaaccttg 661 tattttgtgt ggaaagagtt taccgtgtgc ctgactttgg tgtctgggaa agaggaagca 721 aatataataa tggcagcaca gagctacatt cgagctcggt tggtttagca aaagcagctc 781 tagaagcaat taatggattc aacctttttg gcaaccaggg ctgttcgtgg tcagttatat 841 ttgtggatct cgatgctcac aatcgcaaca ggcaaacttt gtgctcgctg ttacccagag 901 aatcaagatc acataataca gatgatggcc tgctcacctg catcagttat cctgcatttg 961 ccctggatga tgaagttctt tttagccaga cacttgataa agtggttaga aaattaaaag 1021 gaaaatatgg atttaaacgt ttcttgagag atgggtatag aacatcattg gaagatccca 1081 acagatgcta ctacaagcca gctgaaatta agctatttga tggcattgaa tgtgaatttc 1141 ccatattttt cctttatatg atgattgatg gagtttttag aggcaatcct aagcaagtac 1201 aggaatatca ggatcttttg actccagtac ttcatcatac cacagaagga tatcctgttg 1261 taccaaagta ctattatgtg ccagctgact ttgtagaata tgaaaaaaat aaccctggta 1321 gtcaaaaacg atttcctagc aactgtggcc gtgatggaaa actgtttctt tggggacaag 1381 cactttatat catcgcaaaa ctcctggctg atgaacttat tagtcctaaa gacattgatc 1441 ctgtccagcg ctatgtccca ctaaaggatc aacgtaacgt gagcatgagg ttttccaatc 1501 agggcccact ggaaaatgac ttggtagttc atgtggcact tatagcagaa agccaaagac 1561 ttcaagtttt tctgaacaca tatggtattc aaactcaaac tcctcaacaa gtagaaccca 1621 ttcagatatg gcctcagcag gagcttgtga aagcttattt gcagctgggt atcaatgaaa 1681 agttaggact ctctggaagg ccagacaggc ccattggctg cctcgggaca tcaaagattt 1741 atcgcattct aggaaagact gtggtttgtt acccgattat tttcgaccta agtgatttct 1801 acatgtctca ggatgttttc ctgctgatag atgacataaa gaatgcgctg cagttcatta 1861 aacaatattg gaaaatgcat ggacgtccac ttttccttgt tctcatccgg gaagacaata 1921 taagaggtag ccggttcaac cccatattag atatgctggc agcccttaaa aaaggaataa 1981 ttggaggagt caaagttcat gtggatcgtc tacagacact aatatctgga gctgtggtag 2041 aacaacttga tttcctacga atcagtgaca cagaagagct tccagaattt aagagttttg 2101 aggaactaga acctcccaaa cattcaaaag tcaaacggca aagcagcacc cctagtgctc 2161 ctgaactggg acagcagccg gatgtcaaca ttagtgaatg gaaggacaaa cccacccatg 2221 aaattcttca aaaactgaat gattgcagtt gtctggctag ccaagccatc ctgctgggta 2281 tactgctcaa aagagaaggc cccaacttca tcacaaagga aggtaccgtt tctgatcaca 2341 ttgagagagt ctatagaaga gctggcagcc aaaaactttg gtcggttgta cgccgtgcag 2401 caagtctttt aagtaaagta gtggacagcc tggccccatc cattactaat gttttagtgc 2461 agggcaaaca ggtaactctg ggtgcctttg ggcatgaaga agaagttatc tctaatcctt 2521 tgtctccaag agtgattcaa aacatcatct attataagtg taacacccat gatgagaggg 2581 aagcggtcat tcagcaagaa ctggtcatcc atattggctg gatcatctcc aataaccctg 2641 agttattcag tggcatgctg aaaatacgaa tcgggtggat catccatgcc atggagtatg 2701 aacttcagat ccgtggcgga gacaagccag ccttggactt gtatcagctg tcacctagtg 2761 aagttaaaca gcttctgctg gatattctgc agcctcaaca gaatggaaga tgttggctga 2821 acaggcgtca gatcgatggg tctttgaata gaactcccac cgggttctat gaccgagtgt 2881 ggcagattct ggagcgcacg cccaatggga tcattgttgc tgggaagcat ttgcctcagc 2941 aaccaaccct gtcagatatg accatgtatg agatgaattt ctctctcctt gttgaagaca 3001 cgttgggaaa tattGACCAG CCACAGTACA GACAGATCGT TGTAGAGTTA CTTATGGTTG 3061 TATCCATTGT ACTGGAAAGA AACCCCGAGC TAGAATTTCA AGACAAAGTA GATCTAGACA 3121 GACTGGTCAA AGAAGCATTT AATGAATTTC AAAAAGATCA GAGTCGGCTA AAGGAAATTG 3181 AAAAACAAGA TGACATGACT TCCTTTTACA ACACTCCTCC CCTGGGAAAA AGAGGAACAT 3241 GCAGCTATTT GACAAAGGCG GTGATGAATC TGCTGCTGGA AGGAGAAGTC AAGCCAAACA 3301 ATGATGACCC GTGTCTGATT AGCTACCCAA CTTTCTTGTA CAAAGTGGTT GATATCGGTA 3361 AGCCTATCCC TAACCCTCTC CTCGGTCTCG ATTCTACGTA GTAATGAACT AGTCCGTAAC 3421 TTGAAAGTAT TTCGATTTCT TGGCTTTATA TATCTTGTGG AAAGGACGAT GTCCCTAGTT 3481 AATCGCTATT CCAACGCGTT AAGTCgacaa tcaacctctg gattacaaaa tttgtgaaag 3541 att