Transcript: Human NM_000293.3

Homo sapiens phosphorylase kinase regulatory subunit beta (PHKB), transcript variant 1, mRNA.

Source:
NCBI, updated 2019-08-22
Taxon:
Homo sapiens (human)
Gene:
PHKB (5257)
Length:
5459
CDS:
21..3302

Additional Resources:

NCBI RefSeq record:
NM_000293.3
NBCI Gene record:
PHKB (5257)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

Clone ID Target Seq Vector PAM Seq Cut Position Cut % of CDS Length Exon On Target Score[?] Other Matching Genes Orig. Target Gene[?] Notes Addgene[?]
1 BRDN0001149204 TGAGTACCTTTGATGTCCCG pXPR_003 AGG 1682 51% 17 0.258 PHKB PHKB 77846
2 BRDN0001148967 ATCAACGTAACGTGAGCATG pXPR_003 AGG 1440 44% 14 -0.1129 PHKB PHKB 77845
3 BRDN0001144975 TTTCCCACTAAAACATGCGG pXPR_003 TGG 227 7% 3 -0.6581 PHKB PHKB 77847
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shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Human NM_000293.3, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Human Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000197195 GCATGTCATAGCCAATCTAAC pLKO.1 3451 3UTR 100% 10.800 15.120 N PHKB n/a
2 TRCN0000006196 CGCTGCATGTTGTATGATCAA pLKO.1 3581 3UTR 100% 4.950 6.930 N PHKB n/a
3 TRCN0000196396 GCAGCCATTAATATACGAACT pLKO.1 3378 3UTR 100% 4.050 5.670 N PHKB n/a
4 TRCN0000006199 CCTGTCAGATATGACCATGTA pLKO.1 2924 CDS 100% 4.950 3.960 N PHKB n/a
5 TRCN0000194723 CAGCTGAAATTAAGCTATTTG pLKO.1 1075 CDS 100% 13.200 9.240 N PHKB n/a
6 TRCN0000195235 CTCATGATTATGCCAACTATA pLKO.1 3510 3UTR 100% 13.200 9.240 N PHKB n/a
7 TRCN0000196687 GACTGGTCAAAGAAGCATTTA pLKO.1 3097 CDS 100% 13.200 9.240 N PHKB n/a
8 TRCN0000196867 GAACTTCTCTTAGTGGTATTG pLKO.1 3718 3UTR 100% 10.800 7.560 N PHKB n/a
9 TRCN0000195461 GCTTAATCAAGGCAGCCATTA pLKO.1 3367 3UTR 100% 10.800 7.560 N PHKB n/a
10 TRCN0000006198 CCAGAGAATCAAGATCACATA pLKO.1 871 CDS 100% 4.950 3.465 N PHKB n/a
11 TRCN0000006197 GCTCAGTTTATGAACCTCTTA pLKO.1 97 CDS 100% 4.950 3.465 N PHKB n/a
12 TRCN0000006200 CCCAACTTCATCACAAAGGAA pLKO.1 2277 CDS 100% 3.000 2.100 N PHKB n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript NM_000293.3, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_14754 pDONR223 0% 96.8% 96.7% None (many diffs) n/a
2 ccsbBroad304_14754 pLX_304 0% 96.8% 96.7% V5 (many diffs) n/a
3 TRCN0000474290 CTTATGACAAAAGCCCCCCGACTC pLX_317 13.7% 96.8% 96.7% V5 (many diffs) n/a
4 ccsbBroadEn_14753 pDONR223 74.1% 96.7% 96.5% None (many diffs) n/a
5 ccsbBroad304_14753 pLX_304 0% 96.7% 96.5% V5 (many diffs) n/a
6 TRCN0000478777 TGTCCCTAGTTAATCGCTATTCCA pLX_317 9.7% 96.7% 96.5% V5 (many diffs) n/a
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