Construct: ORF TRCN0000479005
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF000090.1_s317c1
- Derived from:
- ccsbBroadEn_05974
- DNA Barcode:
- CGACCGTGTACTGCATGTCGAGAT
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- CDH8 (1006)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000479005
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 1006 | CDH8 | cadherin 8 | NM_001796.5 | 99.9% | 100% | 2211G>A |
| 2 | human | 1006 | CDH8 | cadherin 8 | XM_005255760.4 | 88.1% | 87.7% | (many diffs) |
| 3 | human | 1006 | CDH8 | cadherin 8 | XR_001751811.2 | 51.3% | (many diffs) | |
| 4 | human | 1006 | CDH8 | cadherin 8 | XR_001751813.2 | 49.9% | (many diffs) | |
| 5 | human | 1006 | CDH8 | cadherin 8 | XR_001751812.2 | 22.1% | (many diffs) | |
| 6 | human | 1006 | CDH8 | cadherin 8 | XR_001751814.2 | 21.2% | (many diffs) | |
| 7 | mouse | 12564 | Cdh8 | cadherin 8 | NM_007667.3 | 91.3% | 96.9% | (many diffs) |
| 8 | mouse | 12564 | Cdh8 | cadherin 8 | XM_006530631.2 | 91.3% | 96.9% | (many diffs) |
| 9 | mouse | 12564 | Cdh8 | cadherin 8 | XM_006530634.3 | 83.5% | 84.6% | (many diffs) |
| 10 | mouse | 12564 | Cdh8 | cadherin 8 | NM_001285913.1 | 81.9% | 87.5% | (many diffs) |
| 11 | mouse | 12564 | Cdh8 | cadherin 8 | NM_001039154.2 | 80.7% | 84.1% | (many diffs) |
| 12 | mouse | 12564 | Cdh8 | cadherin 8 | NM_001285914.1 | 80.2% | 84.3% | (many diffs) |
| 13 | mouse | 12564 | Cdh8 | cadherin 8 | NR_133570.1 | 43.2% | (many diffs) | |
| 14 | mouse | 12564 | Cdh8 | cadherin 8 | XM_017312551.1 | 28.9% | 30.9% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 2466
- ORF length:
- 2397
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcaccat gccagaacgg ctagcggaaa tgctcttgga tctctggact ccattaataa 121 tattatggat tactcttccc ccttgcattt acatggctcc gatgaatcag tctcaagttt 181 taatgagtgg atcccctttg gaactaaaca gtctgggtga agaacagcga attttgaacc 241 gctccaaaag aggctgggtt tggaatcaaa tgtttgtcct ggaagagttt tctggacctg 301 aaccgattct tgttggccgg ctacacacag acctggatcc tgggagcaaa aaaatcaagt 361 atatcctatc aggtgatgga gctgggacca tatttcaaat aaatgatgta actggagata 421 tccatgctat aaaaagactt gaccgggagg aaaaggctga gtatacccta acagctcaag 481 cagtggactg ggagacaagc aaacctctgg agcctccttc tgaatttatt attaaagttc 541 aagacatcaa tgacaatgca ccagagtttc ttaatggacc ctatcatgct actgtgccag 601 aaatgtccat tttgggtaca tctgtcacta acgtcactgc gaccgacgct gatgacccag 661 tttatggaaa cagtgcaaag ttggtttata gtatattgga agggcagcct tatttttcca 721 ttgagcctga aacagctatt ataaaaactg cccttcccaa catggacaga gaagccaagg 781 aggagtacct ggttgttatc caagccaaag atatgggtgg acactctggt ggcctgtctg 841 ggaccacgac acttacagtg actcttactg atgttaatga caatcctcca aaatttgcac 901 agagcctgta tcacttctca gtaccggaag atgtggttct tggcactgca ataggaaggg 961 tgaaggccaa tgatcaggat attggtgaaa atgcacagtc atcatatgat atcatcgatg 1021 gagatggaac agcacttttt gaaatcactt ctgatgccca ggcccaggat ggcattataa 1081 ggctaagaaa acctctggac tttgagacca aaaaatccta tacgctaaag gtagaggcag 1141 ccaatgtcca tattgaccca cgcttcagtg gcagggggcc ctttaaagac acggcgacag 1201 tcaaaatcgt ggttgaagat gctgatgagc ctccggtctt ctcttcaccg acttacctac 1261 ttgaagttca tgaaaatgct gctctaaact ccgtgattgg gcaagtgact gctcgtgacc 1321 ctgatatcac ttccagtcct ataaggtttt ccatcgaccg gcacactgac ctggagaggc 1381 agttcaacat taatgcagac gatgggaaga taacgctggc aacaccactt gacagagaat 1441 taagtgtatg gcacaacata acaatcattg ctactgaaat taggaaccac agtcagatat 1501 cacgagtacc tgttgctatt aaagtgctgg atgtcaatga caacgcccct gaattcgcat 1561 ccgaatatga ggcattttta tgtgaaaatg gaaaacccgg ccaagtcatt caaactgtta 1621 gcgccatgga caaagatgat cccaaaaacg gacattattt cttatacagt ctccttccag 1681 aaatggtcaa caatccgaat ttcaccatca agaaaaatga agataattcc ctcagtattt 1741 tggcaaagca taatggattc aaccgccaga agcaagaagt ctatctttta ccaatcataa 1801 tcagtgatag tggaaatcct ccactgagca gcactagcac cttgacaatc agggtctgtg 1861 gctgcagcaa tgacggtgtc gtccagtctt gcaatgtcga agcttatgtc cttccaattg 1921 gactcagtat gggcgcctta attgccatat tagcatgcat cattttgctg ttagtcatcg 1981 tggtgctgtt tgtaactcta cggcggcata aaaatgaacc attaattatc aaagatgatg 2041 aagacgttcg agaaAACATC ATTCGCTACG ATGATGAAGG AGGAGGGGAG GAGGACACAG 2101 AGGCTTTTGA CATTGCAACT TTACAAAATC CAGATGGAAT TAATGGATTT TTACCCCGTA 2161 AGGATATTAA ACCAGATTTG CAGTTTATGC CAAGGCAAGG GCTTGCTCCA GTTCCAAATG 2221 GTGTTGATGT CGATGAATTT ATAAATGTAA GGCTGCATGA GGCAGATAAT GATCCCACAG 2281 CCCCGCCATA TGACTCCATT CAGATATATG GCTATGAAGG CCGAGGGTCA GTGGCTGGCT 2341 CCCTCAGCTC CTTGGAGTCC ACCACATCAG ACTCAGACCA GAATTTTGAC TACCTCAGTG 2401 ACTGGGGTCC CCGCTTTAAG AGACTGGGCG AACTCTACTC TGTTGGTGAA AGTGACAAAG 2461 AAACTTTGCC AACTTTCTTG TACAAAGTGG TTGATATCGG TAAGCCTATC CCTAACCCTC 2521 TCCTCGGTCT CGATTCTACG TAGTAATGAA CTAGTCCGTA ACTTGAAAGT ATTTCGATTT 2581 CTTGGCTTTA TATATCTTGT GGAAAGGACG ACGACCGTGT ACTGCATGTC GAGATACGCG 2641 TTAAGTCgac aatcaacctc tggattacaa aatttgtgaa agatt