Construct: ORF TRCN0000479353
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF009123.1_s317c1
- Derived from:
- ccsbBroadEn_08516
- DNA Barcode:
- TTCGTCTGATTAGCCACAGTGCCA
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- MIS18BP1 (55320)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000479353
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 55320 | MIS18BP1 | MIS18 binding protein 1 | NM_018353.5 | 99.9% | 99.8% | 266C>T;2759G>A |
| 2 | human | 55320 | MIS18BP1 | MIS18 binding protein 1 | XM_005267833.5 | 99.9% | 99.8% | 266C>T;2759G>A |
| 3 | human | 55320 | MIS18BP1 | MIS18 binding protein 1 | XM_017021425.1 | 99.9% | 99.8% | 266C>T;2759G>A |
| 4 | human | 55320 | MIS18BP1 | MIS18 binding protein 1 | XM_011536923.1 | 65.6% | 65.6% | 0_1ins1164;1595G>A |
| 5 | human | 55320 | MIS18BP1 | MIS18 binding protein 1 | XR_002957559.1 | 55.9% | (many diffs) | |
| 6 | human | 55320 | MIS18BP1 | MIS18 binding protein 1 | XR_001750406.2 | 44.6% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 3462
- ORF length:
- 3396
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgat tgcaacacct ttgaaacatt caagaattta cttacctcca gaggcatctt 121 ctcaaaggag aaatctaccc atggatgcaa tcttttttga cagcattcct tcaggcacac 181 ttactcctgt aaaagatttg gtgaaatatc agaactcctc cttaaaattg aatgaccata 241 aaaagaatca gttcctaaaa atgacaactt ttaacaataa aaatatattt caatcaacta 301 tgctaacaga ggctactacc tctaacagtt ttcttgatat cagtgctata aagcccaaca 361 aggatggatt aaaaaataaa gcaaactatg aatcaccagg aaaaatattt ctaagaatga 421 aagaaaaagt actgcgtgac aagcaagaac agccatcaag aaacagtagt ttgttggaac 481 cacagaaaag tggaaataat gaaaccttca ctcctaacag agttgaaaaa aaaaaattgc 541 agcataccta cctatgtgaa gaaaaggaaa acaacaaatc attccagtca gatgacagtt 601 cactaagagc ctcagtccaa ggagttcctc tagaatcatc aaataatgat attttcctcc 661 cggtcaaaca aaagattcag tgccagcagg aaaagaaagc accactgcac aatttaactt 721 acgaacttcc aactctgaac caagaacagg aaaatttttt ggctgtagaa gcccgaaaca 781 agacattaac tagagctcag ttggctaaac aaatttttca ctcaaaggag agtatagttg 841 caaccactaa atccaaaaag gacacgtttg ttttagaaag cgttgattct gctgatgaac 901 aatttcaaaa tactaatgct gagactctca gtactaattg tattcctatt aaaaatggca 961 gcctgttaat ggtttctgat agtgagagga caacagaagg gacttcgcaa cagaaagtta 1021 aggaaggaaa tggaaaaaca gtgcctggag agacaggtct tccaggttcc atgaaagata 1081 catgtaaaat tgtacttgca acaccaagac ttcatataac aatacctcgg aggtcaaaaa 1141 gaaatatttc aaagctttct cctccaagaa tatttcaaac tgttacaaat ggacttaaaa 1201 aaaatcaggt agttcagcta caggaatgga tgattaaaag catcaataat aatactgcta 1261 tatgtgtaga aggaaaattg atagacgtca ctaacatata ttggcacagt aatgtaatta 1321 tagagcggat tgagcacaac aaacttagga ctatatcagg caacgtttat atattaaaag 1381 gcatgataga ccaaatttcc atgaaagaag caggatatcc aaattatctc ataaggaaat 1441 ttatgtttgg atttccagaa aattggaaag agcacattga taattttctg gaacaattaa 1501 gggctggtga aaagaacagg gaaaagacca aacaaaaaca gaaaactgga agatctgtcc 1561 gtgacataag gaaatcaatg aaaaatgatg cacgagaaaa ccaaacagat actgctcaaa 1621 gagccaccac cacttacgat tttgattgtg ataatttgga actgaagagt aataagcaca 1681 gtgagtcacc aggagctaca gaattaaaca tgtgccacag taattgccaa aataaaccaa 1741 cattaaggtt cccagatgac caagtaaata atactattca aaatggagga ggagatgact 1801 tatctaatca ggaattaatt ggaaaaaaag aatataaaat gtcttcaaag aaactaaaaa 1861 ttggtgaaag aacaaatgaa aggataataa aaagtcagaa gcaagagaca actgaagaat 1921 tggatgtatc cattgatatt ctaacctcaa gggaacagtt tttctcagat gaagaaagaa 1981 aatacatggc catcaatcag aagaaagctt atattttagt aacaccactt aaatctagaa 2041 aagtgataga gcaaagatgc atgaggtata atctgtccgc tggcaccatc aaagcagtaa 2101 cagattttgt aataccagag tgtcaaaaaa aaagtcccat cagcaagtcc atggggactt 2161 tagaaaatac atttgaaggt cataaaagta aaaacaagga agattgcgat gaacgtgact 2221 tacttactgt caaccggaaa ataaaaatat ctaaccttga aaaggaacaa atgctcacct 2281 ctgactttaa gaaaaatacc agactattac caaaattgaa gaaaatagaa aatcaggtag 2341 ctatgtcatt ttataagcat cagtcctcac cagatttgtc aagtgaagaa agtgaaacag 2401 aaaaggaaat taaaaggaaa gctgaagtta agaaaaccaa agcaggaaac accaaagaag 2461 cagtggttca cctgagaaag agcacaagaa acacaagtaa tattccagtg attttggaac 2521 ctgaaactga agaaagtgaa aatgaatttt atatcaaaca aaagaaagct agaccttccg 2581 tcaaagaaac tcttcagaag tctggtgtta ggaaagagtt tccaattact gaggcagtag 2641 gatctgataa gacaaatagg catcccttag aatgcttacc tggtttaatt caggataagg 2701 aatggaatga gaaggagtta cagaaacttc attgtgcttt tgcatctctt ccaaagcaca 2761 aacctggttt ctggtcagag gtagctgcgg ctgtaggttc tcgatctcct gaagaatgcc 2821 agaagaaata catggaaaat cccagaggaa aaggatccca gaaacatgtc actaagaaga 2881 agccagccaa ttccaaaggc caaaatggca agagaggtga tgctgatcag aaaCAAACTA 2941 TTAAGATAAC TGCCAAAGTG GGAACTCTTA AAAGGAAGCA ACAGATGAGG GAATTTCTGG 3001 AACAGTTGCC AAAAGATGAC CATGATGATT TTTTCAGTAC AACACCTTTA CAGCATCAAA 3061 GAATACTGTT GCCAAGTTTC CAGGACAGTG AAGATGATGA TGATATTCTG CCAAATATGG 3121 ACAAAAATCC AACAACTCCA TCATCAGTTA TCTTTCCATT GGTAAAAACT CCTCAATGTC 3181 AGCATGTCAG TCCTGGCATG CTAGGTTCTA TAAATAGGAA TGACTGTGAT AAATATGTTT 3241 TTCGTATGCA AAAATATCAT AAAAGTAATG GTGGTATTGT CTGGGGCAAC ATCAAGAAAA 3301 AATTAGTTGA AACTGATTTC TCAACTCCAA CACCAAGAAG GAAAACCCCA TTTAACACAG 3361 ACTTAGGAGA AAACTCTGGT ATTGGAAAAC TTTTCACTAA TGCTGTGGAA TCTTTAGATG 3421 AAGAAGAGAA AGATTATTAT TTTTCGAACT CTGATTCTGC ATACCCAACT TTCTTGTACA 3481 AAGTGGTTGA TATCGGTAAG CCTATCCCTA ACCCTCTCCT CGGTCTCGAT TCTACGTAGT 3541 AATGAACTAG TCCGTAACTT GAAAGTATTT CGATTTCTTG GCTTTATATA TCTTGTGGAA 3601 AGGACGATTC GTCTGATTAG CCACAGTGCC AACGCGTTAA GTCgacaatc aacctctgga 3661 ttacaaaatt tgtgaaagat t