Construct: ORF TRCN0000479398
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF008345.1_s317c1
- Derived from:
- ccsbBroadEn_09284
- DNA Barcode:
- ACTATTTAACACCTTAATACCCGG
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- UBE3B (89910)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000479398
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 89910 | UBE3B | ubiquitin protein ligase E3B | NM_130466.4 | 99.9% | 99.9% | 1037G>A |
| 2 | human | 89910 | UBE3B | ubiquitin protein ligase E3B | NM_183415.3 | 99.9% | 99.9% | 1037G>A |
| 3 | human | 89910 | UBE3B | ubiquitin protein ligase E3B | XM_005253987.2 | 99.9% | 99.9% | 1037G>A |
| 4 | human | 89910 | UBE3B | ubiquitin protein ligase E3B | XM_011538959.2 | 99.9% | 99.9% | 1037G>A |
| 5 | human | 89910 | UBE3B | ubiquitin protein ligase E3B | XM_006719681.3 | 95.5% | 93.9% | (many diffs) |
| 6 | human | 89910 | UBE3B | ubiquitin protein ligase E3B | XM_006719682.2 | 95.5% | 94% | (many diffs) |
| 7 | human | 89910 | UBE3B | ubiquitin protein ligase E3B | XM_011538961.1 | 95.2% | 94% | (many diffs) |
| 8 | human | 89910 | UBE3B | ubiquitin protein ligase E3B | XM_017020195.1 | 81.4% | 80.8% | (many diffs) |
| 9 | human | 89910 | UBE3B | ubiquitin protein ligase E3B | XM_024449269.1 | 81.4% | 80.8% | (many diffs) |
| 10 | human | 89910 | UBE3B | ubiquitin protein ligase E3B | XR_429118.3 | 69.2% | (many diffs) | |
| 11 | human | 89910 | UBE3B | ubiquitin protein ligase E3B | XR_429119.4 | 58% | (many diffs) | |
| 12 | human | 89910 | UBE3B | ubiquitin protein ligase E3B | NM_001270449.1 | 21.6% | 19.3% | (many diffs) |
| 13 | human | 89910 | UBE3B | ubiquitin protein ligase E3B | NM_001270450.1 | 21.6% | 19.3% | (many diffs) |
| 14 | human | 89910 | UBE3B | ubiquitin protein ligase E3B | NM_001270451.1 | 21.6% | 19.3% | (many diffs) |
| 15 | human | 89910 | UBE3B | ubiquitin protein ligase E3B | XM_017020196.1 | 21.6% | 19.3% | (many diffs) |
| 16 | mouse | 117146 | Ube3b | ubiquitin protein ligase E3B | NM_054093.3 | 86% | 92.2% | (many diffs) |
| 17 | mouse | 117146 | Ube3b | ubiquitin protein ligase E3B | XM_006530126.2 | 82.1% | 86.1% | (many diffs) |
| 18 | mouse | 117146 | Ube3b | ubiquitin protein ligase E3B | NM_001331219.1 | 68% | 74.1% | (many diffs) |
| 19 | mouse | 117146 | Ube3b | ubiquitin protein ligase E3B | NR_138602.1 | 53.2% | (many diffs) | |
| 20 | mouse | 117146 | Ube3b | ubiquitin protein ligase E3B | XR_387554.2 | 51.4% | (many diffs) | |
| 21 | mouse | 117146 | Ube3b | ubiquitin protein ligase E3B | XM_011248161.1 | 44.5% | 49.2% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 3273
- ORF length:
- 3204
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcaccat gttcaccctg tctcagacct cgagagcatg gttcatcgat agagcccgtc 121 aggcacgaga agaaaggctt gtgcagaagg aacgggagcg ggcagctgtt gtgatccagg 181 cccatgtccg gagttttctc tgtcggagtc gactgcagag agatatcagg agagagattg 241 atgacttttt taaagcagat gaccctgagt ccactaaaag aagtgcactt tgtattttca 301 agattgccag gaaactgctg ttcctattca gaatcaaaga ggataatgag agatttgaga 361 agttgtgtcg cagcatcctg agcagcatgg atgctgagaa tgagcctaag gtgtggtatg 421 tgtccctggc ttgttctaag gacctcaccc tcctttggat tcaacagatc aagaacattt 481 tgtggtactg ctgtgatttt ctcaagcagc tcaagcctga aatcctgcag gactcccgac 541 tcatcaccct gtacctcacg atgcttgtca ccttcacaga cacttcaacg tggaaaattc 601 ttcggggaaa aggtgaaagt cttcgaccag cgatgaacca catttgtgca aatataatgg 661 gacatctcaa ccagcatgga ttttattctg tgctgcagat attgttaacc cgtggcctgg 721 caagaccccg tccttgtcta tccaaaggca ctttaacagc agctttttct ctagcgttac 781 gccctgtgat tgctgcacag ttctcagaca atctgattcg gccgttcctc atccacatca 841 tgtctgtgcc tgctctggtg actcatctca gcacagtgac ccctgagcgc ctcactgttt 901 tagaatccca tgacatgctt cgtaaattca tcatattttt aagagaccaa gatcgatgcc 961 gtgatgtatg tgaaagttta gaaggatgcc atacgctttg tctaatgggc aacctcctac 1021 acttgggctc cctcagcccc agagtgttag aggaggagac agatgggttc gtgagtttgc 1081 tcacccagac gctgtgctac tgtcagaagt atgtgtctca gaagaagtcc aacctgaccc 1141 actggcatcc tgtccttggc tggttctccc aatctgtgga ttatggcctt aacgagtcaa 1201 tgcacttgat caccaaacag ctgcagttct tgtggggggt gcctctgatc cggatcttct 1261 tctgtgacat cctgagcaag aagctactgg agagccagga gccagcccac gcacagccag 1321 catcccctca gaatgtgctc ccagtgaaga gtctcctaaa gcgtgctttt caaaagtcgg 1381 catcagtccg gaatattctc aggcctgtcg ggggtaaacg ggtcgactct gcagaagtcc 1441 agaaggtttg caacatctgt gtcctctacc agacctcgct gacaactctc acacagattc 1501 ggctgcagat actcacaggt ctcacttacc ttgatgacct gcttcccaaa ctgtgggcat 1561 ttatctgtga gctcgggccc cacggagggt taaagctctt cttggaatgc ctgaacaatg 1621 acactgaaga gtccaagcaa ctcttggcca tgctgatgct gttctgtgac tgttcgcggc 1681 acctcatcac aatccttgat gacattgaag tttatgaaga acagatttca ttcaaactgg 1741 aagagctggt cactatctcc tctttcctga attcttttgt gtttaagatg atctgggatg 1801 gaattgtaga gaacgccaag ggtgagacct tggagctgtt ccagtctgtc cacgggtggc 1861 ttatggtgct gtacgagcgg gactgccggc ggcgcttcac ccccgaggac cactggctgc 1921 gaaaggatct caaacctagc gtgctcttcc aagaactcga cagggacaga aaacgggcac 1981 agttgatcct gcagtacatc ccacatgtca tccctcacaa aaacagagtt ctactgtttc 2041 gaaccatggt taccaaggag aaggagaaac tggggctggt ggaaaccagc tctgcctccc 2101 cgcatgtcac tcacatcacc atccgccggt ccaggatgct ggaggacggc tacgagcagc 2161 ttaggcagct ctcccagcac gccatgaagg gggtcatccg tgtgaagttt gtcaatgacc 2221 tcggggtgga cgaagcaggg attgatcaag acggtgtttt taaggagttc ttggaagaga 2281 tcatcaagag agtttttgac ccagcactca atctgttcaa gacaaccagt ggggatgaga 2341 ggctgtaccc ctcacccaca tcctacatcc atgagaatta cctgcagctc ttcgagtttg 2401 tggggaagat gctggggaag gctgtgtatg agggaattgt ggtggacgtg ccatttgcat 2461 ccttcttcct gagccaactg cttgggcacc accacagcgt cttctatagc tcggtggatg 2521 aactgccttc tctggactcc gagttctata aaaacctcac ctccatcaag cgctatgatg 2581 gggacatcac tgacctgggc ctgacgctgt cttacgacga ggacgtcatg ggtcagcttg 2641 tttgccatga actgattcct ggagggaaga ccattcctgt tacaaatgaa aataaaatta 2701 gctacatcca tctgatggca cattttcgaa tgcacactca aataaaaaac caaacagctg 2761 ccctcattag cggattccgt tccattatca aacccgagtg gatccgaatg ttctcaactc 2821 ctgaactgca gcgtctcatc tctggcgaca atgctgagat tgatctggaa gatttaaaga 2881 agcacacagt ctactacggt ggtttccatg gaagtcacag agtcatcatc tggctctggg 2941 atattctggc cTCCGACTTC ACACCGGATG AGAGAGCTAT GTTTCTGAAG TTCGTGACCA 3001 GCTGCTCCAG ACCCCCGCTC CTGGGATTCG CCTACCTCAA GCCTCCATTC TCCATCCGCT 3061 GCGTGGAGGT GTCGGACGAT CAGGACACCG GGGACACTCT GGGCAGCGTC CTCCGGGGCT 3121 TCTTCACCAT CCGCAAGCGG GAGCCAGGCG GCCGCCTGCC CACCTCCTCC ACCTGCTTCA 3181 ACCTGCTCAA GCTGCCCAAC TACAGCAAGA AGAGCGTCCT CCGCGAGAAG CTGCGCTACG 3241 CCATCAGCAT GAACACGGGC TTTGAACTCT CCTTGCCAAC TTTCTTGTAC AAAGTGGTTG 3301 ATATCGGTAA GCCTATCCCT AACCCTCTCC TCGGTCTCGA TTCTACGTAG TAATGAACTA 3361 GTCCGTAACT TGAAAGTATT TCGATTTCTT GGCTTTATAT ATCTTGTGGA AAGGACGAAC 3421 TATTTAACAC CTTAATACCC GGACGCGTTA AGTCgacaat caacctctgg attacaaaat 3481 ttgtgaaaga tt