Construct: ORF TRCN0000479470
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF017490.1_s317c1
- Derived from:
- ccsbBroadEn_11376
- DNA Barcode:
- ACCGACTGCAGAGTCTGTATAGTC
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- SLC4A8 (9498)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000479470
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 9498 | SLC4A8 | solute carrier family 4 mem... | NM_001258402.2 | 100% | 100% | |
| 2 | human | 9498 | SLC4A8 | solute carrier family 4 mem... | NM_001267615.1 | 92.3% | 92.3% | 0_1ins159 |
| 3 | human | 9498 | SLC4A8 | solute carrier family 4 mem... | XM_006719700.1 | 85.2% | 83.3% | (many diffs) |
| 4 | human | 9498 | SLC4A8 | solute carrier family 4 mem... | NM_001258403.1 | 78.6% | 76.7% | (many diffs) |
| 5 | human | 9498 | SLC4A8 | solute carrier family 4 mem... | XM_017020241.2 | 63% | 61.1% | (many diffs) |
| 6 | human | 9498 | SLC4A8 | solute carrier family 4 mem... | NM_001039960.3 | 62.4% | 61.1% | (many diffs) |
| 7 | human | 9498 | SLC4A8 | solute carrier family 4 mem... | XM_011539010.1 | 61.4% | 60.1% | (many diffs) |
| 8 | human | 9498 | SLC4A8 | solute carrier family 4 mem... | XM_017020240.1 | 59.4% | 55.1% | (many diffs) |
| 9 | human | 9498 | SLC4A8 | solute carrier family 4 mem... | NM_001258401.2 | 57.5% | 56.3% | (many diffs) |
| 10 | human | 9498 | SLC4A8 | solute carrier family 4 mem... | XM_011539011.1 | 56.6% | 55.4% | (many diffs) |
| 11 | human | 9498 | SLC4A8 | solute carrier family 4 mem... | XM_017020242.1 | 56.6% | 55.4% | (many diffs) |
| 12 | human | 9498 | SLC4A8 | solute carrier family 4 mem... | XM_024449282.1 | 56.6% | 55.4% | (many diffs) |
| 13 | human | 9498 | SLC4A8 | solute carrier family 4 mem... | XM_011539014.3 | 17.5% | 16.3% | (many diffs) |
| 14 | mouse | 59033 | Slc4a8 | solute carrier family 4 (an... | NM_021530.2 | 55% | 58.8% | (many diffs) |
| 15 | mouse | 59033 | Slc4a8 | solute carrier family 4 (an... | NM_001347102.1 | 50.5% | 54% | (many diffs) |
| 16 | mouse | 59033 | Slc4a8 | solute carrier family 4 (an... | XM_017316718.1 | 50.5% | 54% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 2139
- ORF length:
- 2073
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgcc ggccgccggg agtaacgagc cggacggcgt cctcagctat cagagaccag 121 atgaagaagc tgtggtggat cagggtggga ccagtacaat tctcaacatt cactatgaaa 181 aagaagagct ggaaggtcac agaactctgt atgtgggagt tcggatgccg cttggccggc 241 agagccatcg gcatcaccgc actcatggcc agaagcaccg gagacgaggg cggggcaaag 301 gagccagcca gggggaggaa ggcctggaag ccctggccca cgacacacca tctcagcgtg 361 ttcagttcat tcttggcacc gaggaagatg aagagcatgt gcctcatgag ctgtttacag 421 agctggatga gatctgtatg aaagagggag aagatgctga gtggaaggaa acagccaggt 481 ggctgaagtt tgaagaagat gttgaagatg ggggagaacg ctggagcaag ccttatgtgg 541 caaccctttc attgcacagc ctgtttgagc taaggagctg ccttattaat ggaacagtcc 601 tcctggatat gcatgcaaat agcatagaag aaatttcaga cctgatcctg gatcagcaag 661 aactgtccag tgacctgaat gacagcatga gggttaaagt gcgggaagcc cttctcaaaa 721 agcatcatca tcagaatgaa aagaagagaa acaacctcat tcccattgtt cgctcctttg 781 ctgaggttgg caagaagcag tctgatcctc atttgatgga taaacatggt caaaccgtgt 841 ctcctcagtc tgttccaact acaaatcttg aagtaaaaaa tggagtgaat tgtgaacata 901 gtcctgtgga tttaagcaag gtagaccttc atttcatgaa aaaaattcct actggggccg 961 aggcctccaa tgtcctggtt ggagaggtgg atattttgga ccgtcccatt gttgcctttg 1021 tgaggctgtc tccagctgtt cttctctcag gcctaacaga agtgccaatc ccaacaagat 1081 ttttgtttat cttattgggt ccagtaggga aaggtcagca gtaccatgag attggcagat 1141 ccatggccac catcatgaca gatgagattt ttcatgacgt agcatataag gcaaaagagc 1201 gagatgatct cctggcgggg attgatgagt tcctagacca ggtgacggtg ctccctccag 1261 gagagtggga tccctccatt agaattgagc cacccaaaaa tgtcccttcc caggagaaaa 1321 ggaaaatgcc tggagttcca aatggaaatg tttgccacat agaacaggaa ccacatgggg 1381 gtcacagtgg gccagaactt cagcgcactg ggcggctatt tgggggcttg gtgctggaca 1441 tcaagcggaa ggccccctgg tactggagcg actaccgaga tgcactcagc ttacagtgtt 1501 tggcttcctt tctgttcctg tactgtgcct gcatgtcacc tgtcatcacc tttgggggac 1561 tgcttggaga agccactgag ggacgcataa gtgcaattga atccttgttt ggagcttcca 1621 tgactgggat tgcttattcc ttgtttgcgg gacaggctct caccatcctg ggaagtactg 1681 gaccagtgct tgtgtttgaa aagattttgt tcaaattctg caaagactat gctctttcat 1741 acctctcccT GCGAGCTTGT ATTGGACTGT GGACCGCTTT CCTGTGTATT GTCCTTGTGG 1801 CAACTGATGC CAGTTCCCTT GTCTGCTACA TTACCCGTTT CACTGAAGAA GCATTTGCCT 1861 CCCTAATTTG CATTATTTTC ATCTATGAAG CAATAGAAAA ACTGATTCAC CTGGCAGAGA 1921 CCTACCCCAT CCACATGCAC AGCCAGCTGG ACCACCTTAG CCTCTATTAC TGCAGGTGTA 1981 CTCTGCCAGA GAATCCAAAC AATCACACCC TCCAGTACTG GAAGGACCAC AACATCGTGA 2041 CAGCAGAAGT CCACTGGGCT AACCTGACTG TCAGTGTAAG TCTGGGAGCT GCCAGATGTC 2101 CTAGCATTGT GACCACAGGT TTAGGAGGGA CTTCTAAGTG CCCAACTTTC TTGTACAAAG 2161 TGGTTGATAT CGGTAAGCCT ATCCCTAACC CTCTCCTCGG TCTCGATTCT ACGTAGTAAT 2221 GAACTAGTCC GTAACTTGAA AGTATTTCGA TTTCTTGGCT TTATATATCT TGTGGAAAGG 2281 ACGAACCGAC TGCAGAGTCT GTATAGTCAC GCGTTAAGTC gacaatcaac ctctggatta 2341 caaaatttgt gaaagatt