Construct: ORF TRCN0000479788
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF006266.1_s317c1
- Derived from:
- ccsbBroadEn_13698
- DNA Barcode:
- CGCTCTCGGCAACTTCTTGCATGC
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- SPDYE7P (441251)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000479788
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 285955 | SPDYE1 | speedy/RINGO cell cycle reg... | XM_017012016.2 | 73.9% | 71.5% | (many diffs) |
| 2 | human | 100505767 | SPDYE18 | speedy/RINGO cell cycle reg... | NM_001351348.1 | 71.8% | 64.5% | (many diffs) |
| 3 | human | 100996746 | SPDYE11 | speedy/RINGO cell cycle reg... | NM_001351349.1 | 70% | 64.5% | (many diffs) |
| 4 | human | 102723849 | SPDYE17 | speedy/RINGO cell cycle reg... | NM_001351351.1 | 70% | 64.5% | (many diffs) |
| 5 | human | 285955 | SPDYE1 | speedy/RINGO cell cycle reg... | XM_017012017.2 | 68.9% | 57.7% | (many diffs) |
| 6 | human | 102723555 | SPDYE16 | speedy/RINGO cell cycle reg... | NM_001351597.1 | 65.4% | 63.1% | (many diffs) |
| 7 | human | 102723555 | SPDYE16 | speedy/RINGO cell cycle reg... | XM_017012887.2 | 65.4% | 63.1% | (many diffs) |
| 8 | human | 388333 | SPDYE4 | speedy/RINGO cell cycle reg... | NM_001128076.3 | 60.3% | 53% | (many diffs) |
| 9 | human | 388333 | SPDYE4 | speedy/RINGO cell cycle reg... | XM_005256633.4 | 60.3% | 53% | (many diffs) |
| 10 | human | 285955 | SPDYE1 | speedy/RINGO cell cycle reg... | NM_175064.2 | 60.1% | 58.1% | (many diffs) |
| 11 | human | 100996746 | SPDYE11 | speedy/RINGO cell cycle reg... | XM_024446630.1 | 59.2% | 48.4% | (many diffs) |
| 12 | human | 441251 | SPDYE7P | speedy/RINGO cell cycle reg... | NR_003666.2 | 58.6% | 1_144del;769_1064del | |
| 13 | human | 285955 | SPDYE1 | speedy/RINGO cell cycle reg... | XM_005249719.3 | 53.7% | 51.9% | (many diffs) |
| 14 | human | 285955 | SPDYE1 | speedy/RINGO cell cycle reg... | XM_017012015.1 | 53.7% | 51.9% | (many diffs) |
| 15 | human | 442590 | SPDYE5 | speedy/RINGO cell cycle reg... | NM_001306141.1 | 50.5% | 48.2% | (many diffs) |
| 16 | human | 441273 | SPDYE2 | speedy/RINGO cell cycle reg... | NM_001031618.3 | 50.2% | 48% | (many diffs) |
| 17 | human | 100310812 | SPDYE2B | speedy/RINGO cell cycle reg... | NM_001166339.1 | 50.2% | 48% | (many diffs) |
| 18 | human | 100310812 | SPDYE2B | speedy/RINGO cell cycle reg... | XM_005250093.4 | 50.2% | 48% | (many diffs) |
| 19 | human | 441273 | SPDYE2 | speedy/RINGO cell cycle reg... | XM_017012223.1 | 50.2% | 48% | (many diffs) |
| 20 | human | 729597 | SPDYE6 | speedy/RINGO cell cycle reg... | NM_001146210.4 | 50.1% | 48% | (many diffs) |
| 21 | human | 100310812 | SPDYE2B | speedy/RINGO cell cycle reg... | XM_011515702.3 | 47.1% | 43.4% | (many diffs) |
| 22 | human | 441273 | SPDYE2 | speedy/RINGO cell cycle reg... | XM_011516237.3 | 47.1% | 43.4% | (many diffs) |
| 23 | human | 441272 | SPDYE3 | speedy/RINGO cell cycle reg... | NM_001004351.5 | 34.4% | 31.4% | (many diffs) |
| 24 | human | 441272 | SPDYE3 | speedy/RINGO cell cycle reg... | XM_011516234.3 | 31.6% | 25.9% | (many diffs) |
| 25 | human | 441272 | SPDYE3 | speedy/RINGO cell cycle reg... | XM_011516235.3 | 31.6% | 25.9% | (many diffs) |
| 26 | human | 641776 | SPDYE14P | speedy/RINGO cell cycle reg... | NR_146072.1 | 24.7% | (many diffs) | |
| 27 | human | 105180390 | SPDYE13P | speedy/RINGO cell cycle reg... | NR_146073.1 | 24.7% | (many diffs) | |
| 28 | human | 105180391 | SPDYE15P | speedy/RINGO cell cycle reg... | NR_146074.1 | 24.7% | (many diffs) | |
| 29 | human | 643862 | SPDYE10P | speedy/RINGO cell cycle reg... | NR_146079.1 | 19.7% | (many diffs) | |
| 30 | human | 728524 | SPDYE8P | speedy/RINGO cell cycle reg... | NR_003664.2 | 19.5% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 693
- ORF length:
- 624
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcaccat ggagtggtgg gacaaatctg aggagtcgtt ggaggaggag ccacggaagg 121 tgctcgcccc tgagcctgag gagatctggg tggcggagat gctgtgtggc ctcaagatga 181 agctgaagcg acggcgagtg tcgctcgtgc tccctgagca ccacgaggcc ttcaacaggc 241 tgcttgagga tcctgtcatt aaaagattcc tggcctggga caaaggtctg agggtgtcgg 301 acaagtatct cctggctatg gtcatagtgt attTCAGCCG GGCCGGCCTC CCCTCCTGGC 361 AATACCAATG CATTCATTTC TTCCTGGCTC TCTACCTGGC CAATGACATG GAGGAGGACG 421 ACGAGGACCC CAAACAAAAC ATCTTCTACT TCCTGTATGG GAAGACCCGC TCTCGCATAC 481 CCTTGCTCCG TAAGCGTCGG TTCCAGTTAT GCCGTTGCAT GAACCCGAGG GCCAGGAAGA 541 ACCGCTCTCA GATAGTCCTG TTCCAGAAAC TTCGGTTCCA GTTCTTCTGT TCCATGAGCT 601 GCAGGGCTTG GGTTTCCCCG GAGGAGTTGG AGGAGATCCA GGCTTATGAC CCAGAGCACT 661 GGGTGTGGGC GCGAGATCGC GCTCGCCTTT CCTTGCCAAC TTTCTTGTAC AAAGTGGTTG 721 ATATCGGTAA GCCTATCCCT AACCCTCTCC TCGGTCTCGA TTCTACGTAG TAATGAACTA 781 GTCCGTAACT TGAAAGTATT TCGATTTCTT GGCTTTATAT ATCTTGTGGA AAGGACGACG 841 CTCTCGGCAA CTTCTTGCAT GCACGCGTTA AGTCgacaat caacctctgg attacaaaat 901 ttgtgaaaga tt