Transcript: Human NR_146072.1

Homo sapiens speedy/RINGO cell cycle regulator family member E14, pseudogene (SPDYE14P), non-coding RNA.

Source:
NCBI, updated 2018-05-09
Taxon:
Homo sapiens (human)
Gene:
SPDYE14P (641776)
Length:
2332
CDS:
(non-coding)

Additional Resources:

NCBI RefSeq record:
NR_146072.1
NBCI Gene record:
SPDYE14P (641776)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the exonic sequence of this non-coding transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Human NR_146072.1, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Human Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000256763 CTTGAGGATCCTGTCATTAAA pLKO_005 546 3UTR 100% 15.000 7.500 Y SPDYE5 n/a
2 TRCN0000161349 GCAATACCAACGCATTCATTT pLKO.1 662 3UTR 100% 13.200 6.600 Y SPDYE2 n/a
3 TRCN0000149464 GCTTGAGGATCCTGTCATTAA pLKO.1 545 3UTR 100% 13.200 6.600 Y SPDYE7P n/a
4 TRCN0000163014 GCTTGAGGATCCTGTCATTAA pLKO.1 545 3UTR 100% 13.200 6.600 Y SPDYE2 n/a
5 TRCN0000337263 TGCTTGAGGATCCTGTCATTA pLKO_005 544 3UTR 100% 13.200 6.600 Y SPDYE6 n/a
6 TRCN0000256760 ATCTCCTGGCTATGGTCATAG pLKO_005 610 3UTR 100% 10.800 5.400 Y SPDYE5 n/a
7 TRCN0000337326 CAATACCAACGCATTCATTTC pLKO_005 663 3UTR 100% 10.800 5.400 Y SPDYE6 n/a
8 TRCN0000427607 TGAGGGTGTCGGACAAGTATC pLKO_005 592 3UTR 100% 10.800 5.400 Y SPDYE1 n/a
9 TRCN0000129780 CAGCAGGAACTTTATTCCAAT pLKO.1 1074 3UTR 100% 4.950 2.475 Y SPDYE7P n/a
10 TRCN0000166211 CAGGAAGAACTGCTCTCAGAT pLKO.1 836 3UTR 100% 4.950 2.475 Y SPDYE8P n/a
11 TRCN0000164079 CATTTCTTCCTGGCTCTCTAT pLKO.1 678 3UTR 100% 4.950 2.475 Y SPDYE8P n/a
12 TRCN0000162810 CCATTCTTGACAGAGCTGAAT pLKO.1 1160 3UTR 100% 4.950 2.475 Y SPDYE8P n/a
13 TRCN0000172267 CTGCTTGAGGATCCTGTCATT pLKO.1 543 3UTR 100% 4.950 2.475 Y SPDYE3 n/a
14 TRCN0000159359 GAATGTTTGGATGAATCTGAT pLKO.1 387 3UTR 100% 4.950 2.475 Y SPDYE8P n/a
15 TRCN0000165864 GATAGCCTTGTTCCGGAAGTA pLKO.1 854 3UTR 100% 4.950 2.475 Y SPDYE8P n/a
16 TRCN0000172571 GTGCCACAGACTGTAACAGAT pLKO.1 1443 3UTR 100% 4.950 2.475 Y SPDYE8P n/a
17 TRCN0000159863 GTTTCTATAAAGCTGTGTGAT pLKO.1 2086 3UTR 100% 4.950 2.475 Y SPDYE1 n/a
18 TRCN0000173018 GTGTTTCCAGTTCCACCCTTT pLKO.1 1263 3UTR 100% 4.050 2.025 Y SPDYE8P n/a
19 TRCN0000165641 GTTGGAAGAGATCCAGGCTTA pLKO.1 929 3UTR 100% 4.050 2.025 Y SPDYE8P n/a
20 TRCN0000164736 CTTGCTCAGTGAGCTTTGGTT pLKO.1 785 3UTR 100% 3.000 1.500 Y SPDYE8P n/a
21 TRCN0000172603 GAACTGCTCTCAGATAGCCTT pLKO.1 842 3UTR 100% 2.640 1.320 Y SPDYE3 n/a
22 TRCN0000165537 GCTCTCATATACCCTTGCTCA pLKO.1 772 3UTR 100% 2.640 1.320 Y SPDYE8P n/a
23 TRCN0000148586 CATTCATTTCTTCCTGGCTCT pLKO.1 674 3UTR 100% 2.160 1.080 Y SPDYE7P n/a
24 TRCN0000163412 GATGAATCTGATGATGAGCCA pLKO.1 396 3UTR 100% 0.660 0.330 Y SPDYE8P n/a
25 TRCN0000166602 CTGGCTATGGTCATAGCGTAT pLKO.1 615 3UTR 100% 0.405 0.203 Y SPDYE2 n/a
26 TRCN0000337319 GGAGGTGGTGGATGATGAAAT pLKO_005 296 3UTR 100% 13.200 6.600 Y SPDYE6 n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript NR_146072.1, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_13656 pDONR223 100% 34% None 1_200del;319T>C;996_2332del n/a
2 ccsbBroad304_13656 pLX_304 0% 34% V5 1_200del;319T>C;996_2332del n/a
3 TRCN0000475668 ACATTGGCACGGGTCGTGATGCCT pLX_317 3.7% 34% V5 1_200del;319T>C;996_2332del n/a
4 ccsbBroadEn_13698 pDONR223 100% 24.7% None (many diffs) n/a
5 ccsbBroad304_13698 pLX_304 0% 24.7% V5 (many diffs) n/a
6 TRCN0000479788 CGCTCTCGGCAACTTCTTGCATGC pLX_317 57.1% 24.7% V5 (many diffs) n/a
7 ccsbBroadEn_13699 pDONR223 100% 22.6% None (many diffs) n/a
8 ccsbBroad304_13699 pLX_304 0% 22.6% V5 (many diffs) n/a
9 TRCN0000491865 TCAAGTGTCGATCAACATATTAAG pLX_317 45.6% 22.6% V5 (many diffs) n/a
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