Construct: ORF TRCN0000479982

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF015375.4_s317c1
Derived from:
ccsbBroadEn_14692
DNA Barcode:
CGCACAATGCGTTGACGATATGGT
Epitope Tag:
V5
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
MAK (4117)

Vector Information:

Vector Backbone:
pLX_317
Pol II Cassette 1:
SV40-PuroR
Pol II Cassette 2:
EF1a-TRCN0000479982
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
V5

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 4117 MAK male germ cell associated k... XM_017010866.2 95.9% 96% 1317_1317delGins55
2 human 4117 MAK male germ cell associated k... XM_024446444.1 86.6% 83.2% (many diffs)
3 human 4117 MAK male germ cell associated k... NM_001242385.1 77% 75.8% (many diffs)
4 human 4117 MAK male germ cell associated k... XM_011514622.3 77% 75.8% (many diffs)
5 human 4117 MAK male germ cell associated k... XR_926220.3 73.5% (many diffs)
6 human 4117 MAK male germ cell associated k... XR_001743419.2 73.3% (many diffs)
7 human 4117 MAK male germ cell associated k... XM_017010865.1 73.3% 69% (many diffs)
8 human 4117 MAK male germ cell associated k... NM_005906.5 72.1% 71% (many diffs)
9 human 4117 MAK male germ cell associated k... XM_017010864.2 72.1% 71% (many diffs)
10 human 4117 MAK male germ cell associated k... NM_001242957.2 69.3% 68.3% (many diffs)
11 human 4117 MAK male germ cell associated k... XM_011514619.2 69.3% 68.3% (many diffs)
12 human 4117 MAK male germ cell associated k... XM_011514620.2 69.3% 68.3% (many diffs)
13 human 4117 MAK male germ cell associated k... XM_017010863.2 69.3% 68.3% (many diffs)
14 human 4117 MAK male germ cell associated k... XM_011514621.2 66% 62.2% (many diffs)
15 human 4117 MAK male germ cell associated k... XR_002956283.1 62.4% (many diffs)
16 human 4117 MAK male germ cell associated k... XR_926215.3 58.4% (many diffs)
17 human 4117 MAK male germ cell associated k... XM_024446443.1 40.3% 39.2% (many diffs)
18 human 4117 MAK male germ cell associated k... NR_134935.2 34.5% 1_266del;1638_3972del
19 human 4117 MAK male germ cell associated k... NR_134936.2 32.1% (many diffs)
20 human 4117 MAK male germ cell associated k... XM_011514624.2 29.3% 28.3% (many diffs)
21 mouse 17152 Mak male germ cell-associated k... XM_006516873.2 63.4% 64.1% (many diffs)
22 mouse 17152 Mak male germ cell-associated k... XM_006516872.2 59.4% 60.1% (many diffs)
23 mouse 17152 Mak male germ cell-associated k... NM_008547.2 59.3% 60% (many diffs)
24 mouse 17152 Mak male germ cell-associated k... XM_006516871.2 59.3% 60% (many diffs)
25 mouse 17152 Mak male germ cell-associated k... XM_006516870.2 57.5% 58.2% (many diffs)
26 mouse 17152 Mak male germ cell-associated k... NM_001145803.1 57.1% 57.8% (many diffs)
27 mouse 17152 Mak male germ cell-associated k... XM_006516868.2 57% 57.7% (many diffs)
28 mouse 17152 Mak male germ cell-associated k... XM_006516869.1 57% 57.7% (many diffs)
Download CSV

Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
66
ORF end:
1437
ORF length:
1371
Sequence:
1tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag
61ttggcatgaa ccgatacaca accatgagac agttggggga cggcacgtat gggagtgtgc
121ttatgggcaa gagtaatgaa tccggggagc tggtggccat caaaaggatg aagagaaagt
181tctattcttg ggatgaatgc atgaacttga gagaagttaa gtctctgaag aaacttaatc
241atgccaatgt tattaaattg aaagaagtta tcagagaaaa tgaccatctt tattttatat
301ttgaatatat gaaagaaaac ctctatcaat taatgaaaga cagaaacaag ttgttccctg
361aatcagtcat cagaaatatt atgtatcaaa tattgcaagg gctggctttt atccataaac
421atggcttttt tcatagggac atgaaaccag aaaacttgct ttgtatgggt ccagagcttg
481tgaaaattgc tgattttgga cttgcaagag aattaaggtc acagccacca tacactgatt
541atgtatctac cagatggtat cgtgcccctg aagttttact gagatcttca gtttatagtt
601ctcccattga tgtgtgggct gttggaagta tcatggctga actctatatg ttaaggccac
661ttttcccagg gacaagtgag gtcgatgaaa tctttaaaat ttgccaagtt ttagggactc
721ccaaaaaaag tgactggcca gaaggatacc agctggcatc ctctatgaac ttccgttttc
781cccagtgtgt tcctataaac ttaaaaactc ttattcccaa tgccagtaat gaagctattc
841agctcatgac cgaaatgttg aattgggatc caaagaaacg accgacagca agccaggcat
901tgaaacaccc atattttcaa gttggtcagg tattaggccc ttcgtcaaat catctggaat
961caaaacagtc tttaaataag cagctgcaac cattagaatc aaagccatct ttagttgagg
1021tagagcctaa gccTCTGCCG GATATAATCG ATCAGGTTGT TGGACAACCC CAGCCAAAAA
1081CTAGCCAGCA GCCACTGCAG CCCATTCAGC CGCCACAGAA CCTGAGCGTC CAGCAACCTC
1141CAAAGCAACA GAGTCAGGAG AAACCGCCAC AAACGCTATT CCCGAGCATC GTCAAAAACA
1201TGCCAACTAA GCCAAATGGC ACACTGAGTC ATAAAAGTGG TAGGAGGCGT TGGGGTCAGA
1261CTATCTTCAA GTCTGGAGAT AGCTGGGAAG AGTTGGAGGA CTATGATTTC GGAGCCTCCC
1321ATTCCAAGAA GCCAAGCATG GGTGTTTTTA AAGAAAAAAG GAAAAAAGAT TCTCCATTTC
1381GTCAACAGGT GAAGATGGCT GTTATTTCCC TCTCAGCACA CCAGTTTCCC ACGTTATGCC
1441CAACTTTCTT GTACAAAGTG GTTGATATCG GTAAGCCTAT CCCTAACCCT CTCCTCGGTC
1501TCGATTCTAC GTAGTAATGA ACTAGTCCGT AACTTGAAAG TATTTCGATT TCTTGGCTTT
1561ATATATCTTG TGGAAAGGAC GACGCACAAT GCGTTGACGA TATGGTACGC GTTAAGTCga
1621caatcaacct ctggattaca aaatttgtga aagatt

Download FASTA (ORF) (Full)