Transcript: Human XM_011514620.2

PREDICTED: Homo sapiens male germ cell associated kinase (MAK), transcript variant X3, mRNA.

Source:
NCBI, updated 2019-09-08
Taxon:
Homo sapiens (human)
Gene:
MAK (4117)
Length:
4831
CDS:
1156..3102

Additional Resources:

NCBI RefSeq record:
XM_011514620.2
NBCI Gene record:
MAK (4117)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

Clone ID Target Seq Vector PAM Seq Cut Position Cut % of CDS Length Exon On Target Score[?] Other Matching Genes Orig. Target Gene[?] Notes Addgene[?]
1 BRDN0001145227 CTGGCCAGAAGGATACCAGC pXPR_003 TGG 685 35% 8 0.712 MAK MAK 76711
2 BRDN0001148291 TGATCGATTATATCCGGCAG pXPR_003 AGG 972 50% 9 -0.3621 MAK MAK 76709
3 BRDN0001146769 ATTCCAGATGATTTGACGAA pXPR_003 GGG 878 45% 9 -0.5720 MAK MAK 76710
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shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Human XM_011514620.2, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Human Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000001786 GATCCACTTAGCACCTCTCAA pLKO.1 2913 CDS 100% 4.950 6.930 N MAK n/a
2 TRCN0000001787 CTCTATATGTTAAGGCCACTT pLKO.1 1732 CDS 100% 4.050 5.670 N MAK n/a
3 TRCN0000001788 CGTCAAATCATCTGGAATCAA pLKO.1 2033 CDS 100% 5.625 4.500 N MAK n/a
4 TRCN0000194697 CTTGATGACTTCAATAGTAAC pLKO.1 4134 3UTR 100% 10.800 7.560 N MAK n/a
5 TRCN0000001789 CAATGCCAGTAATGAAGCTAT pLKO.1 1908 CDS 100% 4.950 3.465 N MAK n/a
6 TRCN0000195056 CGATGGTAATTTCAATCTTGA pLKO.1 3658 3UTR 100% 4.950 3.465 N MAK n/a
7 TRCN0000001785 GCTGTGGTTCTTATTTGACTA pLKO.1 3563 3UTR 100% 4.950 3.465 N MAK n/a
8 TRCN0000194976 CCATCTTTAGTTGAGGTAGAG pLKO.1 2095 CDS 100% 4.050 2.835 N MAK n/a
9 TRCN0000430981 GCCACCATGCCTGGCTAATTT pLKO_005 3835 3UTR 100% 15.000 7.500 Y GTF2IRD2 n/a
10 TRCN0000165534 GAGACAGGGTTTCACCATGTT pLKO.1 3870 3UTR 100% 4.950 2.475 Y n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript XM_011514620.2, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_10956 pDONR223 100% 69.3% 68.3% None (many diffs) n/a
2 ccsbBroad304_10956 pLX_304 0% 69.3% 68.3% V5 (many diffs) n/a
3 TRCN0000469828 TCACTAAAAGCTTCGCAACATTTC pLX_317 30.1% 69.3% 68.3% V5 (many diffs) n/a
4 ccsbBroadEn_14692 pDONR223 0% 69.3% 68.3% None (many diffs) n/a
5 ccsbBroad304_14692 pLX_304 0% 69.3% 68.3% V5 (many diffs) n/a
6 TRCN0000479982 CGCACAATGCGTTGACGATATGGT pLX_317 29.7% 69.3% 68.3% V5 (many diffs) n/a
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