Construct: ORF TRCN0000480030
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF003590.1_s317c1
- Derived from:
- ccsbBroadEn_07140
- DNA Barcode:
- GACGCCAACACATAATGATCTCAC
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- XPC (7508)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000480030
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
---|---|---|---|---|---|---|---|---|
1 | human | 7508 | XPC | XPC complex subunit, DNA da... | NM_004628.4 | 99.9% | 99.8% | 2061G>A;2815C>A |
2 | human | 7508 | XPC | XPC complex subunit, DNA da... | NM_001354729.1 | 99.2% | 99.1% | 85_86insGTGAGGAGGAGGAGGAGG;2043G>A;2797C>A |
3 | human | 7508 | XPC | XPC complex subunit, DNA da... | NM_001354727.2 | 95.9% | 94.9% | (many diffs) |
4 | human | 7508 | XPC | XPC complex subunit, DNA da... | NM_001354730.1 | 91.2% | 91.1% | 1625_1626ins246;1815G>A;2569C>A |
5 | human | 7508 | XPC | XPC complex subunit, DNA da... | NM_001354726.1 | 79.3% | 79.3% | 0_1ins579;1482G>A;2236C>A |
6 | human | 7508 | XPC | XPC complex subunit, DNA da... | NR_148950.2 | 72.7% | (many diffs) | |
7 | human | 7508 | XPC | XPC complex subunit, DNA da... | XR_001740256.2 | 71.7% | (many diffs) | |
8 | human | 7508 | XPC | XPC complex subunit, DNA da... | XR_002959580.1 | 70.4% | (many diffs) | |
9 | human | 7508 | XPC | XPC complex subunit, DNA da... | NR_148951.2 | 69.3% | (many diffs) | |
10 | human | 7508 | XPC | XPC complex subunit, DNA da... | XR_002959581.1 | 53.4% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 2886
- ORF length:
- 2820
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatggc tcggaaacgc gcggccggcg gggagccgcg gggacgcgaa ctgcgcagcc 121 agaaatccaa ggccaagagc aaggcccggc gtgaggagga ggaggaggat gcctttgaag 181 atgagaaacc cccaaagaag agccttctct ccaaagtttc acaaggaaag aggaaaagag 241 gctgcagtca tcctgggggt tcagcagatg gtccagcaaa aaagaaagtg gccaaggtga 301 ctgttaaatc tgaaaacctc aaggttataa aggatgaagc cctcagcgat ggggatgacc 361 tcagggactt tccaagtgac ctcaagaagg cacaccatct gaagagaggg gctaccatga 421 atgaagacag caatgaagaa gaggaagaaa gtgaaaatga ttgggaagag gttgaagaac 481 ttagtgagcc tgtgctgggt gacgtgagag aaagtacagc cttctctcga tctcttctgc 541 ctgtgaagcc agtggagata gagattgaaa cgccagagca ggcgaagaca agagaaagaa 601 gtgaaaagat aaaactggag tttgagacat atcttcggag ggcgatgaaa cgtttcaata 661 aaggggtcca tgaggacaca cacaaggttc accttctctg cctgctagca aatggcttct 721 atcgaaataa catctgcagc cagccagatc tgcatgctat tggcctgtcc atcatcccag 781 cccgctttac cagagtgctg cctcgagatg tggacaccta ctacctctca aacctggtga 841 agtggttcat tggaacattt acagttaatg cagaactttc agccagtgaa caagataacc 901 tgcagactac attggaaagg agatttgcta tttactctgc tcgagatgat gaggaattgg 961 tccatatatt cttactgatt ctccgggctc tgcagctctt gacccggctg gtattgtctc 1021 tacagccaat tcctctgaag tcagcaacag caaagggaaa gaaaccttcc aaggaaagat 1081 tgactgcgga tccaggaggc tcctcagaaa cttccagcca agttctagaa aaccacacca 1141 aaccaaagac cagcaaagga accaaacaag aggaaacctt tgctaagggc acctgcaggc 1201 caagtgccaa agggaagagg aacaagggag gcagaaagaa acggagcaag ccctcctcca 1261 gcgaggaaga tgagggccca ggagacaagc aggagaaggc aacccagcga cgtccgcatg 1321 gccgggagcg gcgggtggcc tccagggtgt cttataaaga ggagagtggg agtgatgagg 1381 ctggcagcgg ctctgatttt gagctctcca gtggagaagc ctctgatccc tctgatgagg 1441 attccgaacc tggccctcca aagcagagga aagcccccgc tcctcagagg acaaaggctg 1501 ggtccaagag tgcctccagg acccatcgtg ggagccatcg taaggaccca agcttgccag 1561 cggcatcctc aagctcttca agcagtaaaa gaggcaagaa aatgtgcagc gatggtgaga 1621 aggcagaaaa aagaagcata gctggtatag accagtggct agaggtgttc tgtgagcagg 1681 aggaaaagtg ggtatgtgta gactgtgtgc acggtgtggt gggccagcct ctgacctgtt 1741 acaagtacgc caccaagccc atgacctatg tggtgggcat tgacagtgac ggctgggtcc 1801 gagatgtcac acagaggtac gacccagtct ggatgacagt gacccgcaag tgccgggttg 1861 atgctgagtg gtgggccgag accttgagac cataccagag cccatttatg gacagggaga 1921 agaaagaaga cttggagttt caggcaaaac acatggacca gcctttgccc actgccattg 1981 gcttatataa gaaccaccct ctgtatgccc tgaagcggca tctcctgaaa tatgaggcca 2041 tctatcccga gacagctgcc atccttgggt attgtcgtgg agaagcggtc tactccaggg 2101 attgtgtgca cactctgcat tccagagaca cgtggctgaa gaaagcaaga gtggtgaggc 2161 ttggagaagt accctacaag atggtgaaag gcttttctaa ccgtgctcgg aaagcccgac 2221 ttgctgagcc ccagctgcgg gaagaaaatg acctgggcct gtttggctac tggcagacag 2281 aggagtatca gcccccagtg gccgtggacg ggaaggtgcc ccggaacgag tttgggaatg 2341 tgtacctctt cctgcccagc atgatgccta ttggctgtgt ccagctgaac ctgcccaatc 2401 tacaccgcgt ggcccgcaag ctggacatcg actgtgtcca ggccatcact ggctttgatt 2461 tccatggcgg ctactcccat cccgtgactg atggatacat cgtctgcgag gaattcaaag 2521 acgtgctcct gactgcctgg GAAAATGAGC AGGCAGTCAT TGAAAGGAAG GAGAAGGAGA 2581 AAAAGGAGAA GCGGGCTCTA GGGAACTGGA AGTTGCTGGC CAAAGGTCTG CTCATCAGGG 2641 AGAGGCTGAA GCGTCGCTAC GGGCCCAAGA GTGAGGCAGC AGCTCCCCAC ACAGATGCAG 2701 GAGGTGGACT CTCTTCTGAT GAAGAGGAGG GGACCAGCTC TCAAGCAGAA GCGGCCAGGA 2761 TACTGGCTGC CTCCTGGCCT CAAAACCGAG AAGATGAAGA AAAGCAGAAG CTGAAGGGTG 2821 GGCCCAAGAA GACCAAAAGG GAAAAGAAAG CAGCAGCTTC CCACCTGTTC CCATTTGAGA 2881 AGCTGTGCCC AACTTTCTTG TACAAAGTGG TTGATATCGG TAAGCCTATC CCTAACCCTC 2941 TCCTCGGTCT CGATTCTACG TAGTAATGAA CTAGTCCGTA ACTTGAAAGT ATTTCGATTT 3001 CTTGGCTTTA TATATCTTGT GGAAAGGACG AGACGCCAAC ACATAATGAT CTCACACGCG 3061 TTAAGTCgac aatcaacctc tggattacaa aatttgtgaa agatt