Construct: ORF TRCN0000480062
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF010358.1_s317c1
- Derived from:
- ccsbBroadEn_05061
- DNA Barcode:
- TGCATCGCAGAGCTACCTAGCGTC
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- RNF38 (152006)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000480062
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 152006 | RNF38 | ring finger protein 38 | NM_194328.2 | 100% | 100% | |
| 2 | human | 152006 | RNF38 | ring finger protein 38 | NM_194330.2 | 100% | 100% | |
| 3 | human | 152006 | RNF38 | ring finger protein 38 | NM_194332.2 | 100% | 100% | |
| 4 | human | 152006 | RNF38 | ring finger protein 38 | XM_005251366.3 | 100% | 100% | |
| 5 | human | 152006 | RNF38 | ring finger protein 38 | XM_005251367.3 | 100% | 100% | |
| 6 | human | 152006 | RNF38 | ring finger protein 38 | XM_006716721.3 | 100% | 100% | |
| 7 | human | 152006 | RNF38 | ring finger protein 38 | XM_011517712.2 | 100% | 100% | |
| 8 | human | 152006 | RNF38 | ring finger protein 38 | XM_011517713.2 | 100% | 100% | |
| 9 | human | 152006 | RNF38 | ring finger protein 38 | XM_017014294.1 | 94.1% | 84.3% | (many diffs) |
| 10 | human | 152006 | RNF38 | ring finger protein 38 | NM_194329.3 | 92.9% | 92.9% | 1_99del |
| 11 | human | 152006 | RNF38 | ring finger protein 38 | XM_017014296.1 | 92.6% | 91.8% | (many diffs) |
| 12 | human | 152006 | RNF38 | ring finger protein 38 | XM_017014297.1 | 92.6% | 91.8% | (many diffs) |
| 13 | human | 152006 | RNF38 | ring finger protein 38 | NM_022781.5 | 83.8% | 83.8% | 1_249del |
| 14 | mouse | 73469 | Rnf38 | ring finger protein 38 | NM_001038993.3 | 93.2% | 98.1% | (many diffs) |
| 15 | mouse | 73469 | Rnf38 | ring finger protein 38 | XM_006538302.3 | 93.2% | 98.1% | (many diffs) |
| 16 | mouse | 73469 | Rnf38 | ring finger protein 38 | XM_006538303.3 | 93.2% | 98.1% | (many diffs) |
| 17 | mouse | 73469 | Rnf38 | ring finger protein 38 | XM_006538304.3 | 93.2% | 98.1% | (many diffs) |
| 18 | mouse | 73469 | Rnf38 | ring finger protein 38 | XM_006538305.3 | 93.2% | 98.1% | (many diffs) |
| 19 | mouse | 73469 | Rnf38 | ring finger protein 38 | XM_006538306.3 | 93.2% | 98.1% | (many diffs) |
| 20 | mouse | 73469 | Rnf38 | ring finger protein 38 | XM_011250115.2 | 93.2% | 98.1% | (many diffs) |
| 21 | mouse | 73469 | Rnf38 | ring finger protein 38 | XM_011250116.2 | 93.2% | 98.1% | (many diffs) |
| 22 | mouse | 73469 | Rnf38 | ring finger protein 38 | XM_011250117.2 | 93.2% | 98.1% | (many diffs) |
| 23 | mouse | 73469 | Rnf38 | ring finger protein 38 | XM_011250118.2 | 93.2% | 98.1% | (many diffs) |
| 24 | mouse | 73469 | Rnf38 | ring finger protein 38 | XM_011250119.2 | 93.2% | 98.1% | (many diffs) |
| 25 | mouse | 73469 | Rnf38 | ring finger protein 38 | XM_011250120.2 | 93.2% | 98.1% | (many diffs) |
| 26 | mouse | 73469 | Rnf38 | ring finger protein 38 | NM_175201.5 | 86.8% | 91.3% | (many diffs) |
| 27 | mouse | 73469 | Rnf38 | ring finger protein 38 | XM_006538301.3 | 83.9% | 88.3% | (many diffs) |
| 28 | mouse | 73469 | Rnf38 | ring finger protein 38 | XM_006538300.3 | 83% | 87.4% | (many diffs) |
| 29 | mouse | 73469 | Rnf38 | ring finger protein 38 | XM_006538299.3 | 78.4% | 82.4% | (many diffs) |
| 30 | mouse | 73469 | Rnf38 | ring finger protein 38 | XM_006538298.3 | 69.8% | 73.4% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1362
- ORF length:
- 1296
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgcg accatgggag atgacatcaa ataggcagcc cccttcagtt cgaccaagcc 121 aacatcactt ctcaggggaa cgatgcaaca cacctgcacg caacagaaga agtcctcctg 181 tcaggcgcca gagaggaaga agggatcgtc tgtctcgaca taattccatt agtcaagatg 241 aaaactatca ccatctccct tacgcacagc agcaagcaat agaggagcct cgagccttcc 301 accctccgaa tgtatctccc cgtctgctac atcctgctgc tcatccaccc cagcagaatg 361 cagtcatggt tgacatacat gatcagctcc atcaaggaac agtccctgtt tcttacacag 421 taacaacagt ggcaccacat gggattccac tctgcacagg ccagcacatc cctgcttgta 481 gtacacagca ggtcccagga tgctctgtgg ttttcagtgg acagcacctc cctgtctgta 541 gtgtgcctcc tccaatgctt caggcatgtt cagttcagca cttaccagta ccatatgctg 601 cattcccacc ccttatttct agtgatccat ttcttataca tcctcctcac ctttctcccc 661 atcatcctcc tcatttgcca ccaccaggcc agtttgtccc tttccaaaca cagcaatcac 721 gatcgcctct gcaaaggata gaaaatgaag tggaactctt aggagaacat cttccagtag 781 gaggttttac ttaccctcca tcagcccacc ccccaacatt acctccatca gctcccttgc 841 agttcttaac acatgatcct ttgcatcagg aggtgtcctt tggagtacct tatcctccat 901 ttatgcctcg gaggcttaca ggacgtagta gataccgatc ccagcagcca ataccacctc 961 ccccttatca tcccagctta ctgccatatg tgttatcaat gcttccagtg ccaccTGCAG 1021 TGGGCCCAAC TTTCAGCTTT GAATTAGATG TAGAAGATGG AGAAGTAGAA AATTACGAGG 1081 CCCTGTTAAA CCTGGCAGAG CGACTGGGAG AGGCAAAGCC TCGTGGACTG ACTAAAGCAG 1141 ATATTGAACA ACTTCCTTCT TATCGGTTCA ATCCTAACAA CCACCAGTCA GAACAGACTT 1201 TGTGTGTAGT ATGCATGTGT GATTTTGAGT CAAGGCAGCT ACTTAGAGTC TTACCCTGTA 1261 ACCACGAGTT CCATGCCAAG TGTGTTGACA AATGGCTTAA GGCAAATCGT ACTTGCCCAA 1321 TTTGCCGAGC TGATGCTTCA GAAGTGCATC GGGATTCAGA ATGCCCAACT TTCTTGTACA 1381 AAGTGGTTGA TATCGGTAAG CCTATCCCTA ACCCTCTCCT CGGTCTCGAT TCTACGTAGT 1441 AATGAACTAG TCCGTAACTT GAAAGTATTT CGATTTCTTG GCTTTATATA TCTTGTGGAA 1501 AGGACGATGC ATCGCAGAGC TACCTAGCGT CACGCGTTAA GTCgacaatc aacctctgga 1561 ttacaaaatt tgtgaaagat t