Construct: ORF TRCN0000480068
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF000159.1_s317c1
- Derived from:
- ccsbBroadEn_08495
- DNA Barcode:
- AGGCTCTTTTCCCGTCTAAGCATA
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- UQCC1 (55245)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000480068
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 55245 | UQCC1 | ubiquinol-cytochrome c redu... | NM_018244.5 | 99.8% | 99.6% | 152G>A |
| 2 | human | 55245 | UQCC1 | ubiquinol-cytochrome c redu... | XM_011528877.1 | 95.4% | 95.2% | 127_168del;194G>A |
| 3 | human | 55245 | UQCC1 | ubiquinol-cytochrome c redu... | NM_199487.2 | 91.1% | 90.9% | 152G>A;572_573ins78 |
| 4 | human | 55245 | UQCC1 | ubiquinol-cytochrome c redu... | XM_011528878.2 | 89.2% | 89.2% | 127_128ins96 |
| 5 | human | 55245 | UQCC1 | ubiquinol-cytochrome c redu... | XM_011528879.1 | 84.5% | 84.2% | 0_1ins138;14G>A |
| 6 | human | 55245 | UQCC1 | ubiquinol-cytochrome c redu... | XM_011528880.2 | 84.5% | 84.2% | 0_1ins138;14G>A |
| 7 | human | 55245 | UQCC1 | ubiquinol-cytochrome c redu... | NM_001184977.1 | 77.2% | 77.2% | 128_129ins204 |
| 8 | human | 55245 | UQCC1 | ubiquinol-cytochrome c redu... | XM_011528881.3 | 66.8% | 66.8% | 0_1ins297 |
| 9 | human | 55245 | UQCC1 | ubiquinol-cytochrome c redu... | XM_011528882.2 | 54.6% | 54.5% | 0_1ins405;2_4delTGG |
| 10 | human | 55245 | UQCC1 | ubiquinol-cytochrome c redu... | XM_011528883.2 | 54.6% | 54.5% | 0_1ins405;2_4delTGG |
| 11 | human | 55245 | UQCC1 | ubiquinol-cytochrome c redu... | XM_011528884.2 | 54.6% | 54.5% | 0_1ins405;2_4delTGG |
| 12 | mouse | 56046 | Uqcc1 | ubiquinol-cytochrome c redu... | NM_018888.4 | 87% | 87.2% | (many diffs) |
| 13 | mouse | 56046 | Uqcc1 | ubiquinol-cytochrome c redu... | XM_011239704.2 | 81.6% | 79.6% | (many diffs) |
| 14 | mouse | 56046 | Uqcc1 | ubiquinol-cytochrome c redu... | XM_011239707.2 | 41.7% | 37.6% | (many diffs) |
| 15 | mouse | 56046 | Uqcc1 | ubiquinol-cytochrome c redu... | NR_024487.2 | 29.1% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 966
- ORF length:
- 897
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcaccat ggcgttgctg gtgcgagtcc ttaggaacca gactagcatt tctcagtggg 121 ttccagtatg cagccgattg atacctgtgt ctcctaccca aggacagggg gacagggctc 181 tgtctcgcac ttcccagtgg ccccagatga gccagtccca agcatgtggt ggatcagaac 241 agattcctgg aatagacata cagctgaata ggaagtatca caccacacgt aagctttcta 301 ctaccaaaga ttccccacag cctgttgagg agaaggttgg tgctttcaca aagataatag 361 aagccatggg attcacggga cctttgaaat acagtaaatg gaagattaag attgcggccc 421 tgcgcatgta tactagctgt gtggagaaaa ctgacttcga ggaattcttt ctaaggtgtc 481 agatgcctga tacattcaat tcatggtttc ttataaccct actccacgtc tggatgtgtc 541 tagtccgaat gaagcaggaa ggccggagtg ggaagtacat gtgtcgtatc atagttcatt 601 ttatgtggga ggatgttcag cagcgcggca gagtcatggg ggttaatccc tatatcctga 661 agaagaacat gatcctcatg acaaatcatt tctatgcagc gatcttggga tatgatgagg 721 ggatcctttc agatgatcat gggctggccg ctgccctctg gagaaccttc ttcaaccgga 781 aatgtgaaga ccctcgacat CTTGAATTGC TGGTAGAGTA TGTGAGGAAA CAGATACAGT 841 ACCTGGACTC CATGAACGGG GAGGATCTGC TTCTGACAGG GGAGGTGAGC TGGCGCCCTC 901 TAGTGGAGAA GAATCCTCAG AGCATCCTGA AGCCCCATTC TCCGACTTAC AACGACGAGG 961 GACTTTTGCC AACTTTCTTG TACAAAGTGG TTGATATCGG TAAGCCTATC CCTAACCCTC 1021 TCCTCGGTCT CGATTCTACG TAGTAATGAA CTAGTCCGTA ACTTGAAAGT ATTTCGATTT 1081 CTTGGCTTTA TATATCTTGT GGAAAGGACG AAGGCTCTTT TCCCGTCTAA GCATAACGCG 1141 TTAAGTCgac aatcaacctc tggattacaa aatttgtgaa agatt