Construct: ORF TRCN0000480095
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF017005.1_s317c1
- Derived from:
- ccsbBroadEn_11496
- DNA Barcode:
- ACAACAACATTCGCGCAAGGGGGT
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- TUBGCP3 (10426)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000480095
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 10426 | TUBGCP3 | tubulin gamma complex assoc... | NM_001286279.1 | 98.8% | 98.8% | 1197_1211del |
| 2 | human | 10426 | TUBGCP3 | tubulin gamma complex assoc... | XM_005268293.2 | 51.4% | 49.1% | (many diffs) |
| 3 | human | 10426 | TUBGCP3 | tubulin gamma complex assoc... | NM_001286278.2 | 51% | 48.7% | (many diffs) |
| 4 | human | 10426 | TUBGCP3 | tubulin gamma complex assoc... | XM_011537462.2 | 50.1% | 45.2% | (many diffs) |
| 5 | human | 10426 | TUBGCP3 | tubulin gamma complex assoc... | NM_006322.6 | 46.3% | 44.3% | (many diffs) |
| 6 | human | 10426 | TUBGCP3 | tubulin gamma complex assoc... | NM_001286277.2 | 45.2% | 43.2% | (many diffs) |
| 7 | human | 10426 | TUBGCP3 | tubulin gamma complex assoc... | XR_001749455.2 | 30.7% | (many diffs) | |
| 8 | human | 10426 | TUBGCP3 | tubulin gamma complex assoc... | XM_017020323.2 | 21.1% | 19% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1368
- ORF length:
- 1302
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatggc gaccccggac cagaagtcgc cgaacgttct gctgcagaac ctgtgctgca 121 ggatcctggg caggagcgaa gctgatgtag cccagcagtt ccagtatgct gtgcgggtga 181 ttggcagcaa cttcgcccca actgttgaaa gagatgaatt tttagtagct gaaaaaatca 241 agaaagagct tattcgacaa cgaagagaag cagatgctgc attattttca gaactccaca 301 gaaaacttca ttcacaggga gttttgaaaa ataaatggtc aatactctac ctcttgctga 361 gcctcagtga ggacccacgc aggcagccaa gcaaggtttc tagctatgct acgttatttg 421 ctcaggcctt accaagagat gcccactcaa ccccttacta ctatgccagg cctcagaccc 481 ttcccctgag ctaccaagat cggagtgccc agtcagccca gagctccggc agcgtgggca 541 gcagtggcat cagcagcatt ggcctgtgtg ccctcagtgg ccccgcgcct gcgccacaat 601 ctctcctccc aggacagtct aatcaagctc caggagtagg agattgcctt cgacagcagt 661 tggggtcacg actcgcatgg actttaactg caaatcagcc ttcttcacaa gccactacct 721 caaaaggtgt ccccagtgct gtgtctcgca acatgacaag gtccaggaga gaaggggata 781 cgggtggtac tatggaaatt acagaagcag ctctggtaag ggacattttg tacgtctttc 841 agggcataga tggcaaaaac atcaaaatga acaacactga aaattgttac aaagtagaag 901 gaaaggcaaa tctaagtagg tctttgagag acacagcagt caggctttct gagttgggat 961 ggttgcataa taaaaTCAGA AGATACACGG ACCAGAGGAG CCTGGACCGC TCATTCGGAC 1021 TCGTCGGGCA GAGCTTTTGT GCTGCCTTGC ACCAGGAACT CAGAGAATAC TATCGATTGC 1081 TCTCTGTTTT ACATTCTCAG CTACAACTAG AGGATGACCA GGGTGTGAAT TTGGGACTTG 1141 AGAGTAGTTT AACACTTCGG CGCCTCCTGG TTTGGACCTA TGATCCCAAA ATACGACTGA 1201 AGACCCTTGC GGCCCTAGTG GACCACTGCC AAGGTCCCAC GCGCGTCTTT CCCACGCACG 1261 TCTTTCCCAC GCGCGACTTT CCCACGCGCG ACTTTCCCAT GCACGTCTTT CCCACGCGCG 1321 TCTTTCCCAC GCGCGTCTGG CACTCGCTTT GTTTCAGAAC TCGTTTGTAC CCAACTTTCT 1381 TGTACAAAGT GGTTGATATC GGTAAGCCTA TCCCTAACCC TCTCCTCGGT CTCGATTCTA 1441 CGTAGTAATG AACTAGTCCG TAACTTGAAA GTATTTCGAT TTCTTGGCTT TATATATCTT 1501 GTGGAAAGGA CGAACAACAA CATTCGCGCA AGGGGGTACG CGTTAAGTCg acaatcaacc 1561 tctggattac aaaatttgtg aaagatt