Construct: ORF TRCN0000480176

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF014392.2_s317c1
Derived from:
ccsbBroadEn_09684
DNA Barcode:
TTCCTACGGGACCTCAATCGAACG
Epitope Tag:
V5
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
IGSF11 (152404)

Vector Information:

Vector Backbone:
pLX_317
Pol II Cassette 1:
SV40-PuroR
Pol II Cassette 2:
EF1a-TRCN0000480176
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
V5

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 152404 IGSF11 immunoglobulin superfamily ... NM_001353320.2 99.8% 99.5% 112C>A;1282T>A
2 human 152404 IGSF11 immunoglobulin superfamily ... NM_152538.4 99.8% 99.5% 112C>A;1282T>A
3 human 152404 IGSF11 immunoglobulin superfamily ... XM_011512469.1 99.8% 99.5% 112C>A;1282T>A
4 human 152404 IGSF11 immunoglobulin superfamily ... NM_001015887.3 97.4% 95.8% (many diffs)
5 human 152404 IGSF11 immunoglobulin superfamily ... XM_024453376.1 95% 93.4% (many diffs)
6 human 152404 IGSF11 immunoglobulin superfamily ... NM_001353322.2 94.2% 93.7% 112C>A;628_629ins72;1210T>A
7 human 152404 IGSF11 immunoglobulin superfamily ... NM_001353321.2 91.9% 90.1% (many diffs)
8 human 152404 IGSF11 immunoglobulin superfamily ... NM_001353323.2 91% 89.1% (many diffs)
9 human 152404 IGSF11 immunoglobulin superfamily ... XM_011512463.1 89.4% 88.9% 50_199del;262C>A;1432T>A
10 human 152404 IGSF11 immunoglobulin superfamily ... XM_011512464.2 89.4% 88.9% 50_199del;262C>A;1432T>A
11 human 152404 IGSF11 immunoglobulin superfamily ... XM_011512465.2 89.4% 88.9% 50_199del;262C>A;1432T>A
12 human 152404 IGSF11 immunoglobulin superfamily ... NM_001353318.2 87.5% 86% (many diffs)
13 human 152404 IGSF11 immunoglobulin superfamily ... NM_001353325.2 86.4% 86.2% 0_1ins174;1108T>A
14 human 152404 IGSF11 immunoglobulin superfamily ... XM_017005789.1 86.4% 86.2% 0_1ins174;1108T>A
15 human 152404 IGSF11 immunoglobulin superfamily ... NM_001353324.2 85.5% 83.9% (many diffs)
16 human 152404 IGSF11 immunoglobulin superfamily ... XM_011512466.2 82.5% 80.8% (many diffs)
17 human 152404 IGSF11 immunoglobulin superfamily ... NM_001353319.2 81.7% 80% (many diffs)
18 human 152404 IGSF11 immunoglobulin superfamily ... NM_001353326.2 80.8% 80.4% 0_1ins174;454_455ins72;1036T>A
19 human 152404 IGSF11 immunoglobulin superfamily ... XM_011512471.3 76.8% 75.2% (many diffs)
20 human 152404 IGSF11 immunoglobulin superfamily ... XM_024453377.1 57.4% 54.7% (many diffs)
21 human 152404 IGSF11 immunoglobulin superfamily ... XM_011512474.3 51.7% 48.8% (many diffs)
22 mouse 207683 Igsf11 immunoglobulin superfamily,... NM_170599.2 84.9% 85.4% (many diffs)
23 mouse 207683 Igsf11 immunoglobulin superfamily,... XM_006521892.2 83.8% 86.4% (many diffs)
24 mouse 207683 Igsf11 immunoglobulin superfamily,... XM_006521893.2 83.6% 86.5% (many diffs)
25 mouse 207683 Igsf11 immunoglobulin superfamily,... XM_011245851.1 74.4% 77.9% (many diffs)
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Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
66
ORF end:
1356
ORF length:
1290
Sequence:
1tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag
61ttggcatgtc tctggtggaa cttttgctct ggtggaactg cttttctaga actggtgttg
121cagcatccct ggaagtgtca gagagccctg ggagtatcca ggtggcccgg ggtcagacag
181cagtcctgcc ctgcactttc actaccagcg ctgccctcat taacctcaat gtcatttgga
241tggtcactcc tctctccaat gccaaccaac ctgaacaggt catcctgtat cagggtggac
301agatgtttga tggtgccccc cggttccacg gtagggtagg atttacaggc accatgccag
361ctaccaatgt ctctatcttc attaataaca ctcagttatc agacactggc acctaccagt
421gcctggtcaa caaccttcca gacatagggg gcaggaacat tggggtcacc ggtctcacag
481tgttagttcc cccttctgcc ccacactgcc aaatccaagg atcccaggat attggcagcg
541atgtcatcct gctctgtagc tcagaggaag gcattcctcg accaacttac ctttgggaga
601agttagacaa taccctcaaa ctacctccaa cagctactca ggaccaggtc cagggaacag
661tcaccatccg gaacatcagt gccctgtctt caggtttgta ccagtgcgtg gcttctaatg
721ctattggaac cagcacctgt cttctggatc tccaggttat ttcaccccag cccaggaaca
781ttggactaat agctggagcc attggcactg gtgcagttat tatcattttt tgcattgcac
841taattttagg ggcattcttt tactggagaa gcaaaaataa agaggaggaa gaagaagaaa
901ttcctaatga aataagagag gatgatcttc cacccaagtg ttcttctgcc aaagcatttc
961acactgagat ttcctcctcg gacaacaaca cactaacctc ttccaatgcc tacaacagtc
1021gatactGGAG CAACAATCCA AAAGTTCATA GAAACACAGA GTCAGTCAGC CACTTCAGTG
1081ACTTGGGCCA ATCTTTCTCT TTCCACTCAG GCAATGCCAA CATACCATCC ATTTATGCTA
1141ATGGGACCCA TCTGGTCCCG GGTCAACATA AGACTCTGGT AGTGACAGCC AACAGAGGGT
1201CATCACCACA GGTGATGTCC AGGAGCAATG GCTCAGTCAG TAGGAAGCCT CGGCCTCCAC
1261ACACTCATTC CTACACCATC AGCCACGCAA CACTGGAACG AATTGGTGCA GTACCTGTCA
1321TGGTACCAGC CCAGAGTCGG GCCGGGACCT TGGTATACCC AACTTTCTTG TACAAAGTGG
1381TTGATATCGG TAAGCCTATC CCTAACCCTC TCCTCGGTCT CGATTCTACG TAGTAATGAA
1441CTAGTCCGTA ACTTGAAAGT ATTTCGATTT CTTGGCTTTA TATATCTTGT GGAAAGGACG
1501ATTCCTACGG GACCTCAATC GAACGACGCG TTAAGTCgac aatcaacctc tggattacaa
1561aatttgtgaa agatt

Download FASTA (ORF) (Full)