Construct: ORF TRCN0000480285
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF016214.1_s317c1
- Derived from:
- ccsbBroadEn_09619
- DNA Barcode:
- TGTAGCTGACGCTCAGAAAATGGG
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- ARMH4 (145407)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000480285
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 145407 | ARMH4 | armadillo like helical doma... | NM_001001872.4 | 99.9% | 99.7% | 287C>T;1837C>G |
| 2 | human | 145407 | ARMH4 | armadillo like helical doma... | XM_017021029.1 | 99.9% | 99.7% | 287C>T;1837C>G |
| 3 | human | 145407 | ARMH4 | armadillo like helical doma... | XM_017021030.2 | 99.7% | 99.6% | 287C>T;1837C>G;2254_2255insAGC |
| 4 | human | 145407 | ARMH4 | armadillo like helical doma... | NM_001320173.2 | 82.6% | 80.6% | (many diffs) |
| 5 | human | 145407 | ARMH4 | armadillo like helical doma... | XM_011536471.3 | 80.9% | 78.2% | (many diffs) |
| 6 | human | 145407 | ARMH4 | armadillo like helical doma... | XR_943399.3 | 54.8% | (many diffs) | |
| 7 | human | 145407 | ARMH4 | armadillo like helical doma... | XR_943398.3 | 54.7% | (many diffs) | |
| 8 | human | 145407 | ARMH4 | armadillo like helical doma... | XR_943395.2 | 23.8% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 2391
- ORF length:
- 2322
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcaccat gagaggaccg attgtattgc acatttgtct ggctttctgt agccttctgc 121 ttttcagcgt tgccacacaa tgtctggcct tccccaaaat agaaaggagg agggagatag 181 cacatgttca tgcggaaaaa gggcagtccg ataagatgaa caccgatgac ctagaaaata 241 gctctgttac ctcaaagcag actccccaac tggtggtctc tgaagatcca atgatgatgt 301 cagcagtacc atcggcaaca tcattaaata aagcattctc gattaacaaa gaaatccagc 361 ctggacaagc tgggctcatg caaacagaac gccctggtgt ttccacacct actgagtcag 421 gtgtcccctc agctgaagaa gtatttggtt ccagccagcc agagagaata tctcctgaaa 481 gtggacttgc caaggccatg ttaaccattg ctatcactgc gactccttct ctgactgttg 541 atgaaaagga ggaactcctt acaagcacta actttcagcc cattgtagaa gagatcacag 601 aaaccacaaa aggttttctg aagtatatgg ataatcaatc atttgcaact gaaagtcagg 661 aaggagttgg tttgggacat tcaccttcat cctatgtgaa tactaaggaa atgctaacca 721 ccaatccaaa gactgagaaa tttgaagcag acacagacca caggacaact tcttttcctg 781 gtgctgagtc cacagcaggc agtgagcctg gaagcctcac ccctgataag gagaagcctt 841 cgcagatgac agctgataac acccaggctg ctgccaccaa gcaaccactc gaaacttccg 901 agtacaccct gagtgttgag ccagaaactg atagtctgct gggagcccca gaagtcacag 961 tgagtgtcag cacagctgtt ccagctgcct ctgccttaag tgatgagtgg gatgacacca 1021 aattagagag tgtaagccgg ataaggaccc ccaagcttgg agacaatgaa gagactcagg 1081 tgagaacgga gatgtctcag acagcacaag taagccatga gggtatggaa ggaggccagc 1141 cttggacaga ggctgcacag gtggctctgg ggctgcctga aggggaaaca cacacgggca 1201 cagccctgct aatagcgcat gggaatgaga gatcacctgc tttcactgat caaagttcct 1261 ttacccccac aagtctgatg gaagacatga aagtttccat tgtgaacttg ctccaaagta 1321 cgggagactt cacggaatcc accaaggaaa acgatgccct gtttttctta gaaaccactg 1381 tttctgtctc tgtatatgag tctgaggcag accaactgtt gggaaataca atgaaagaca 1441 tcatcactca agagatgaca acagctgttc aagagccaga tgccacttta tccatggtga 1501 cacaagagca ggttgctacc ctcgagctta tcagagacag tggcaagact gaggaagaaa 1561 aggaggaccc ctctcctgtg tctgacgttc ctggtgttac tcagctgtca agaagatggg 1621 agcctctggc cactacaatt tcaactacag tcgtcccttt gtcttttgaa gttactccca 1681 ctgtggaaga acaaatggac acagtcacag ggccaaatga ggagttcaca ccagttctgg 1741 gatctccagt gacacctcct ggaataatgg tgggggaacc cagcatttcc cctgcacttc 1801 ctgctttgga ggcatcctct gagagaagaa ctgttgttcc atctattact cgtgttaata 1861 cagctgcctc atatggcctg gaccaacttg aatctgaaga gggagaagaa gatgaggatg 1921 aagaggatga agaagatgaa gatgaagaag aggaagatga ggaagaagat gaggaagata 1981 aagatgcaga ctcgctggat gagggcttgg atggtgaCAC TGAGCTGCCA GGTTTTACCC 2041 TCCCTGGTAT CACATCCCAG GAACCAGGCT TAGAGGAGGG AAACATGGAC CTGTTGGAGG 2101 GAGCTACCTA CCAGGTGCCA GATGCCCTCG AGTGGGAACA GCAGAATCAA GGCCTGGTGA 2161 GAAGCTGGAT GGAAAAATTA AAAGACAAGG CTGGTTACAT GTCTGGGATG CTGGTGCCTG 2221 TAGGGGTTGG GATAGCTGGA GCCTTGTTCA TCTTGGGAGC CCTCTACAGC ATTAAGGTTA 2281 TGAATCGCCG AAGGAGAAAT GGCTTCAAAA GGCATAAAAG AAAGCAGAGA GAATTCAACA 2341 GCATGCAAGA TCGAGTAATG CTCTTAGCCG ACAGCTCTGA AGATGAATTT TTGCCAACTT 2401 TCTTGTACAA AGTGGTTGAT ATCGGTAAGC CTATCCCTAA CCCTCTCCTC GGTCTCGATT 2461 CTACGTAGTA ATGAACTAGT CCGTAACTTG AAAGTATTTC GATTTCTTGG CTTTATATAT 2521 CTTGTGGAAA GGACGATGTA GCTGACGCTC AGAAAATGGG ACGCGTTAAG TCgacaatca 2581 acctctggat tacaaaattt gtgaaagatt