Construct: ORF TRCN0000480322
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF012280.1_s317c1
- Derived from:
- ccsbBroadEn_15453
- DNA Barcode:
- AAACGCCAACCAAGTTGCGGTACA
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- AGFG1 (3267)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000480322
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 3267 | AGFG1 | ArfGAP with FG repeats 1 | NM_004504.4 | 99.8% | 100% | 270A>G;1416A>G |
| 2 | human | 3267 | AGFG1 | ArfGAP with FG repeats 1 | NM_001135188.1 | 99.5% | 99.6% | 270A>G;1416A>G;1536_1537insGGTGCA |
| 3 | human | 3267 | AGFG1 | ArfGAP with FG repeats 1 | XM_006712480.3 | 97.1% | 97.2% | 270A>G;1378_1425del;1464A>G |
| 4 | human | 3267 | AGFG1 | ArfGAP with FG repeats 1 | XM_005246516.4 | 95.7% | 95.7% | 270A>G;815_886del;1488A>G |
| 5 | human | 3267 | AGFG1 | ArfGAP with FG repeats 1 | NM_001135187.1 | 95.4% | 95.3% | (many diffs) |
| 6 | human | 3267 | AGFG1 | ArfGAP with FG repeats 1 | XM_006712478.4 | 93.2% | 93.1% | (many diffs) |
| 7 | human | 3267 | AGFG1 | ArfGAP with FG repeats 1 | XM_006712479.4 | 92.9% | 92.8% | (many diffs) |
| 8 | human | 3267 | AGFG1 | ArfGAP with FG repeats 1 | NM_001135189.1 | 92.7% | 92.8% | 270A>G;691_692ins120;1296A>G |
| 9 | human | 3267 | AGFG1 | ArfGAP with FG repeats 1 | XM_017003954.1 | 59.9% | 59.4% | (many diffs) |
| 10 | human | 3267 | AGFG1 | ArfGAP with FG repeats 1 | XM_006712481.3 | 56% | 55.3% | (many diffs) |
| 11 | mouse | 15463 | Agfg1 | ArfGAP with FG repeats 1 | XM_006496433.3 | 92.7% | 96.7% | (many diffs) |
| 12 | mouse | 15463 | Agfg1 | ArfGAP with FG repeats 1 | XM_006496434.3 | 92.5% | 96.7% | (many diffs) |
| 13 | mouse | 15463 | Agfg1 | ArfGAP with FG repeats 1 | NM_010472.3 | 92.3% | 96.4% | (many diffs) |
| 14 | mouse | 15463 | Agfg1 | ArfGAP with FG repeats 1 | XM_006496435.3 | 92.2% | 96.4% | (many diffs) |
| 15 | mouse | 15463 | Agfg1 | ArfGAP with FG repeats 1 | XM_006496430.3 | 90.1% | 94.1% | (many diffs) |
| 16 | mouse | 15463 | Agfg1 | ArfGAP with FG repeats 1 | XM_006496431.3 | 90% | 94.1% | (many diffs) |
| 17 | mouse | 15463 | Agfg1 | ArfGAP with FG repeats 1 | XM_006496432.3 | 89.7% | 93.7% | (many diffs) |
| 18 | mouse | 15463 | Agfg1 | ArfGAP with FG repeats 1 | NM_001310713.1 | 87.4% | 91.6% | (many diffs) |
| 19 | mouse | 15463 | Agfg1 | ArfGAP with FG repeats 1 | XM_006496438.3 | 87.3% | 91.6% | (many diffs) |
| 20 | mouse | 15463 | Agfg1 | ArfGAP with FG repeats 1 | XM_006496439.3 | 87.1% | 91.2% | (many diffs) |
| 21 | mouse | 15463 | Agfg1 | ArfGAP with FG repeats 1 | XM_006496440.3 | 85.7% | 89.8% | (many diffs) |
| 22 | mouse | 15463 | Agfg1 | ArfGAP with FG repeats 1 | NM_001347077.1 | 83.3% | 87.3% | (many diffs) |
| 23 | mouse | 15463 | Agfg1 | ArfGAP with FG repeats 1 | XM_017316914.1 | 82.9% | 87% | (many diffs) |
| 24 | mouse | 15463 | Agfg1 | ArfGAP with FG repeats 1 | XR_373537.3 | 56.9% | (many diffs) | |
| 25 | mouse | 15463 | Agfg1 | ArfGAP with FG repeats 1 | XR_001781822.1 | 18.5% | (many diffs) | |
| 26 | mouse | 15463 | Agfg1 | ArfGAP with FG repeats 1 | XR_001781825.1 | 18.5% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1752
- ORF length:
- 1686
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatggc ggccagcgcg aagcggaagc aggaggagaa gcacctgaag atgctgcggg 121 acatgaccgg cctcccgcac aaccgaaagt gcttcgactg cgaccagcgc ggccccacct 181 acgttaacat gacggtcggc tccttcgtgt gtacctcctg ctccggcagc ctgcgaggat 241 taaatccacc acacagggtg aaatctatct ccatgacaac attcacacaa caggaaattg 301 aattcttaca aaaacatgga aatgaagtct gtaagcagat ttggctagga ttatttgatg 361 atagatcttc agcaattcca gacttcaggg atccacaaaa agtgaaagag tttctacaag 421 aaaagtatga aaagaaaaga tggtatgtcc cgccagaaca agccaaagtc gtggcatcag 481 ttcatgcatc tatttcaggg tcctctgcca gtagcacaag cagcacacct gaggtcaaac 541 cactgaaatc tcttttaggg gattctgcac caacactgca cttaaataag ggcacaccta 601 gtcagtcccc agttgtaggt cgttctcaag ggcagcagca ggagaagaag caatttgacc 661 ttttaagtga tctcggctca gacatctttg ctgctccagc tcctcagtca acagctacag 721 ccaattttgc taactttgca catttcaaca gtcatgcagc tcagaattct gcaaatgcag 781 attttgcaaa ctttgatgca tttggacagt ctagtggttc gagtaatttt ggaggtttcc 841 ccacagcaag tcactctcct tttcagcccc aaactacagg tggaagtgct gcatcagtaa 901 atgctaattt tgctcatttt gataacttcc ccaaatcctc cagtgctgat tttggaacct 961 tcaatacttc ccagagtcat caaacagcat cagctgttag taaagtttca acgaacaaag 1021 ctggtttaca gactgcagac aaatatgcag cacttgctaa tttagacaat atcttcagtg 1081 ccgggcaagg tggtgatcag ggaagtggct ttgggaccac aggtaaagct cctgttggtt 1141 ctgtggtttc agttcccagt cagtcaagtg catcttcaga caagtatgca gctctggcag 1201 aactagacag cgttttcagt tctgcagcca cctccagtaa tgcgtatact tccacaagta 1261 atgctagcag caatgttttt ggaacagtgc cagtggttgc ttctgcacag acacagcctg 1321 cttcatcaag tgtgcctgct ccatttggag ctacgccTTC CACAAATCCA TTTGTTGCTG 1381 CTGCTGGTCC TTCTGTGGCA TCTTCTACAA ACCCATTTCA GACCAATGCC AGAGGAGCAA 1441 CAGCGGCAAC CTTTGGCACT GCATCCATGA GCATGCCCAC GGGATTCGGC ACTCCTGCTC 1501 CCTACAGTCT TCCCACCAGC TTTAGTGGCA GCTTTCAGCA GCCTGCCTTT CCAGCCCAAG 1561 CAGCTTTCCC TCAACAGACA GCTTTTTCTC AACAGCCCAA TGGTGCAGGT TTTGCAGCAT 1621 TTGGACAAAC AAAGCCAGTA GTAACCCCTT TTGGTCAAGT TGCAGCTGCT GGAGTATCTA 1681 GTAATCCTTT TATGACTGGT GCACCAACAG GACAATTTCC AACAGGAAGC TCATCAACCA 1741 ATCCTTTCTT ATACCCAACT TTCTTGTACA AAGTGGTTGA TATCGGTAAG CCTATCCCTA 1801 ACCCTCTCCT CGGTCTCGAT TCTACGTAGT AATGAACTAG TCCGTAACTT GAAAGTATTT 1861 CGATTTCTTG GCTTTATATA TCTTGTGGAA AGGACGAAAA CGCCAACCAA GTTGCGGTAC 1921 AACGCGTTAA GTCgacaatc aacctctgga ttacaaaatt tgtgaaagat t