Construct: ORF TRCN0000480690
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF017764.2_s317c1
- Derived from:
- ccsbBroadEn_01930
- DNA Barcode:
- ACCCTTATATAATGGCCCACTCCA
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- CUL2 (8453)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000480690
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 8453 | CUL2 | cullin 2 | NM_001198777.2 | 100% | 100% | |
| 2 | human | 8453 | CUL2 | cullin 2 | NM_003591.4 | 100% | 100% | |
| 3 | human | 8453 | CUL2 | cullin 2 | XM_011519747.1 | 100% | 100% | |
| 4 | human | 8453 | CUL2 | cullin 2 | NM_001198779.1 | 98.2% | 98.2% | 1_39del |
| 5 | human | 8453 | CUL2 | cullin 2 | NM_001198778.2 | 97.5% | 97.5% | 1_57del |
| 6 | human | 8453 | CUL2 | cullin 2 | XM_011519743.1 | 92.2% | 92.2% | 1_189del |
| 7 | human | 8453 | CUL2 | cullin 2 | XM_011519744.1 | 92.2% | 92.2% | 1_189del |
| 8 | human | 8453 | CUL2 | cullin 2 | XM_011519745.1 | 92.2% | 92.2% | 1_189del |
| 9 | human | 8453 | CUL2 | cullin 2 | NM_001324375.2 | 91.5% | 91.4% | 317_318ins189 |
| 10 | human | 8453 | CUL2 | cullin 2 | NM_001324376.2 | 82.4% | 82.4% | 0_1ins393 |
| 11 | mouse | 71745 | Cul2 | cullin 2 | NM_029402.3 | 91.3% | 97.3% | (many diffs) |
| 12 | mouse | 71745 | Cul2 | cullin 2 | XM_006526274.3 | 91.3% | 97.3% | (many diffs) |
| 13 | mouse | 71745 | Cul2 | cullin 2 | XM_006526275.3 | 91.3% | 97.3% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 2301
- ORF length:
- 2235
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgtc tttgaaacca agagtagtag attttgatga aacatggaac aaacttttga 121 cgacaataaa agccgtggtc atgttggaat acgtcgaaag agcaacatgg aatgaccgtt 181 tctcagatat ctatgcttta tgtgtggcct atcctgaacc ccttggagaa agactttata 241 cagaaactaa gatttttttg gaaaatcatg ttcggcattt gcataagaga gttttggagt 301 cagaagaaca agtacttgtt atgtatcata ggtactggga agaatacagc aagggtgcag 361 actatatgga ctgcttatat aggtatctca acacccagtt tattaaaaag aataaattaa 421 cagaagcgga ccttcagtat ggctatggtg gtgtagatat gaatgaacca cttatggaaa 481 taggagagct agcattggat atgtggagga aattgatggt tgaaccactt caggccatcc 541 ttatccgaat gctgctccga gaaatcaaaa atgatcgtgg tggagaagac ccaaaccaga 601 aagtaatcca tggggttatt aactcctttg ttcatgttga acagtataag aaaaaattcc 661 ccttaaagtt ttatcaggaa atttttgagt ctccctttct gactgaaaca ggagagtatt 721 acaaacaaga agcttcaaat ttattacaag aatcaaactg ctcacagtat atggaaaagg 781 ttctaggtag attaaaagat gaagaaattc gatgtcgaaa atacctacat ccaagttcat 841 atactaaggt gattcatgaa tgtcaacaac gaatggtagc agaccactta cagtttttac 901 atgcagaatg tcataatata attcgacaag agaaaaaaaa tgacatggca aatatgtacg 961 tcttactccg tgctgtgtcc actggtttac ctcatatgat tcaggagctg caaaaccaca 1021 tccatgatga gggccttcga gcaaccagca accttactca ggaaaacatg ccaacactat 1081 ttgtggagtc agttttggaa gtgcatggta aatttgttca gcttatcaac actgttttga 1141 atggtgatca gcattttatg agtgcgttgg ataaggccct tacgtcagtt gtaaattaca 1201 gagaacctaa gtctgtttgc aaagcacctg aactgcttgc taagtactgt gacaacttac 1261 tgaagaagtc agcgaaaggg atgacagaga atgaagtgga agacaggctc acgagcttca 1321 tcacagtgtt caaatacatt gatgacaagg acgtctttca aaagttctac gcaagaatgc 1381 tggcaaaacg tttaattcat gggttatcca tgtctatgga ctctgaagaa gccatgatca 1441 acaaattaaa gcaagcctgt ggttatgagt ttaccagcaa gctacatcgg atgtatacag 1501 atatgagtgt cagcgctgat ctcaacaata agttcaacaa ttttatcaaa aaccaagaca 1561 cagtaataga tttgggaatt agttttcaaa tatatgttct acaggctggt gcgtggcctc 1621 ttactcaggc tccttcatct acgtttgcaa ttccccagga attagaaaaa agtgtacaga 1681 tgtttgaatt attttatagc caacatttca gtggaaggaa acttacatgg ttacattatc 1741 tgtgtacagg tgaagttaaa atgaactatt tgggcaaacc atatgtagcc atggttacaa 1801 cataccaaat ggcagttctt cttgccttta acaacagtga aactgtcagt tataaagagc 1861 ttcaggacag cactcagatg aatgaaaagg aactgacaaa aaCAATCAAA TCATTACTTG 1921 ATGTGAAAAT GATTAACCAT GATTCAGAAA AGGAAGATAT TGATGCAGAA TCTTCGTTTT 1981 CATTAAATAT GAACTTTAGC AGTAAAAGAA CAAAATTTAA AATTACTACA TCAATGCAGA 2041 AAGACACACC ACAAGAAATG GAGCAGACTA GAAGTGCAGT TGATGAGGAC CGGAAAATGT 2101 ATCTCCAAGC TGCTATAGTT CGTATCATGA AAGCACGAAA AGTGCTTCGG CACAATGCCC 2161 TTATTCAAGA GGTGATTAGC CAGTCAAGAG CTAGGTTTAA TCCCAGTATC AGCATGATTA 2221 AGAAGTGTAT TGAAGTTCTG ATAGACAAAC AATACATAGA ACGCAGCCAG GCGTCGGCAG 2281 ATGAATACAG CTACGTCGCG TGCCCAACTT TCTTGTACAA AGTGGTTGAT ATCGGTAAGC 2341 CTATCCCTAA CCCTCTCCTC GGTCTCGATT CTACGTAGTA ATGAACTAGT CCGTAACTTG 2401 AAAGTATTTC GATTTCTTGG CTTTATATAT CTTGTGGAAA GGACGAACCC TTATATAATG 2461 GCCCACTCCA ACGCGTTAAG TCgacaatca acctctggat tacaaaattt gtgaaagatt