Construct: ORF TRCN0000480695
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF013779.1_s317c1
- Derived from:
- ccsbBroadEn_15518
- DNA Barcode:
- TTTATGGTGTACGTAAATGCAATC
- Epitope Tag:
- V5 (not translated due to prior stop codon)
- Notes:
- Early stop codon detected
Originally Annotated References:
- Gene:
- PCDHGC3 (5098)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000480695
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 5098 | PCDHGC3 | protocadherin gamma subfami... | NM_002588.4 | 99.3% | 86.1% | (many diffs) |
| 2 | human | 5098 | PCDHGC3 | protocadherin gamma subfami... | NM_032402.2 | 89.3% | 94% | (many diffs) |
| 3 | human | 5098 | PCDHGC3 | protocadherin gamma subfami... | NM_032403.3 | 13.4% | .9% | (many diffs) |
| 4 | mouse | 93706 | Pcdhgc3 | protocadherin gamma subfami... | NM_033581.3 | 87.4% | 78.4% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 2562
- ORF length:
- 2496
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatggt cccagaggcc tggaggagcg gactggtaag caccgggagg gtagtgggag 121 ttttgcttct gcttggtgcc ttgaacaagg cttccacggt cattcactat gagatcccgg 181 aggaaagaga gaagggtttc gctgtgggca acgtggtcgc gaaccttggt ttggatctcg 241 gtagcctctc agcccgcagg ttccgggtgg tgtctggagc tagccgaaga ttctttgagg 301 tgaaccggga gaccggagag atgtttgtga acgaccgtct ggatcgagag gagctgtgtg 361 ggacactgcc ctcttgcact gtaactctgg agttggtagt ggagaacccg ctggagctgt 421 tcagcgtgga agtggtgatc caggacatca acgacaacaa tcctgctttc cctacccagg 481 aaatgaaatt ggagattagc gaggccgtgg ctccggggac gcgctttccg ctcgagagcg 541 cgcacgatcc cgatgtggga agcaactctt tacaaaccta tgagctgagc cgaaatgaat 601 actttgcgct tcgcgtgcag acgcgggagg acagcaccaa gtacgcggag ctggtgttgg 661 agcgcgccct ggaccgagaa cgggagccta gtctccagtt agtgctgacg gcgttggacg 721 gagggacccc agctctctcc gccagcctgc ctattcacat caaggtgctg gacgcgaatg 781 acaatgcgcc tgtcttcaac cagtccttgt accgggcgcg cgtcctggag gatgcaccct 841 ccggcacgcg cgtggtacaa gtccttgcaa cggatctgga tgaaggcccc aacggtgaaa 901 ttatttactc cttcggcagc cacaaccgcg ccggcgtgcg gcaactattc gccttagacc 961 ttgtaaccgg gatgctgaca atcaagggtc ggctggactt cgaggacacc aaactccatg 1021 agatttacat ccaggccaaa gacaagggcg ccaatcccga aggagcacat tgcaaagtgt 1081 tggtggaggt tgtggatgtg aatgacaacg ccccggagat cacagtcacc tccgtgtaca 1141 gcccagtacc cgaggatgcc cctctgggga ctgtcatcgc tttgctcagt gtgactgacc 1201 tggatgctgg cgagaacggg ctggtgacct gcgaagttcc accgggtctc cctttcagcc 1261 ttacttcttc cctcaagaat tacttcactt tgaaaaccag tgcagacctg gatcgggaga 1321 ctgtgccaga atacaacctc agcatcaccg cccgagacgc cggaacccct tccctctcag 1381 cccttacaat agtgcgtgtt caagtgtccg acatcaatga caaccctcca caatcttctc 1441 aatcttccta cgacgtttac attgaagaaa acaacctccc cggggctcca atactaaacc 1501 taagtgtctg ggaccccgac gccccgcaga atgctcggct ttctttcttt ctcttggagc 1561 aaggagctga aaccgggcta gtgggtcgct atttcacaat aaatcgtgac aatggcatag 1621 tgtcatcctt agtgccccta gactatgagg atcggcggga atttgaatta acagctcata 1681 tcagcgatgg gggcaccccg gtcctagcca ccaacatcag cgtgaacata tttgtcactg 1741 atcgcaatga caatgccccc caggtcctat atcctcggcc aggtgggagc tcggtggaga 1801 tgctgcctcg aggtacctca gctggccacc tagtgtcacg ggtggtaggc tgggacgcgg 1861 atgcagggca caatgcctgg ctctcctaca gtctcttggg atcccctaac cagagccttt 1921 ttgccatagg gctgcacact ggtcaaatca gtactgcccg tccagtccaa gacacagatt 1981 cacccaggca gactctcacg gtcttgatca aagacaatgg ggagccttcg ctctccacca 2041 ctgctaccct cactgtgtca gtaaccgagg actctcctga agcccgagcc gagttcccct 2101 ctggctctgc cccccgggag cagaaaaaaa atctcacctt ttatctactt ctttctctaa 2161 tcctggtttc tgtggggttt gtggtcacag tgttcggagt aatcatattc aaagtttaca 2221 agtggaagca gtctagagac ctataccgag ccccggtgag ctcactgtac cgaacaccag 2281 ggccctcctt gcacgcggac gccgtgcggg gaggcctgat gtcgccgcac ctttaccatc 2341 aggtgtatct caccacggac tcccgccgca gcgacccgct gctgaagaaa cctggtgcag 2401 ccagtccact ggccagccgc cagaacacgc tgcggagctg tgatccggtg ttctataggc 2461 aggtgttggg tgcagagagc gcccctcccg gacagcaagc cccgcACAAA AAAGTTGGCA 2521 TGGTCCCAGA GGCCTGGCAC CAGCGGCTCC CAAAATGGCG ATGACACCGG CACCTGGCCC 2581 AACAACCAGT TTGACACAGA GATGCTGCAA GCCATGATCT TGGCGTCCGC CAGTGAAGCT 2641 GCTGATGGGA GCTCCACCCT GGGAGGGGGT GCCGGCACCA TGGGATTGAG CGCCCGCTAC 2701 GGACCCCAGT TCACCCTGCA GCACGTGCCC GACTACCGCC AGAATGTCTA CATCCCAGGC 2761 AGCAATGCCA CACTGACCAA CGCAGCTGGC AAGCGGGATG GCAAGGCCCC AGCAGGTGGC 2821 AATGGCAACA AGAAGAAGTC GGGCAAGAAG GAGAAGAAGT AACCAACTTT CTTGTACAAA 2881 GTGGTTGATA TCGGTAAGCC TATCCCTAAC CCTCTCCTCG GTCTCGATTC TACGTAGTAA 2941 TGAACTAGTC CGTAACTTGA AAGTATTTCG ATTTCTTGGC TTTATATATC TTGTGGAAAG 3001 GACGATTTAT GGTGTACGTA AATGCAATCA CGCGTTAAGT Cgacaatcaa cctctggatt 3061 acaaaatttg tgaaagatt