Construct: ORF TRCN0000480783
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF004223.1_s317c1
- Derived from:
- ccsbBroadEn_08818
- DNA Barcode:
- GGCGCCCTCACTTTTCACATATCA
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- CXorf56 (63932)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000480783
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 63932 | CXorf56 | chromosome X open reading f... | NM_022101.4 | 99.8% | 100% | 375C>T |
| 2 | human | 63932 | CXorf56 | chromosome X open reading f... | NM_001170570.2 | 93.5% | 93.6% | 242_243ins42;333C>T |
| 3 | human | 63932 | CXorf56 | chromosome X open reading f... | NM_001170569.1 | 77.7% | 77.9% | 0_1ins147;228C>T |
| 4 | human | 728024 | LOC728024 | chromosome X open reading f... | NR_003671.2 | 43.1% | (many diffs) | |
| 5 | mouse | 77644 | C330007P06Rik | RIKEN cDNA C330007P06 gene | NM_029951.1 | 91.8% | 99.5% | (many diffs) |
| 6 | mouse | 77644 | C330007P06Rik | RIKEN cDNA C330007P06 gene | XM_006541410.3 | 83.3% | 87.9% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 732
- ORF length:
- 666
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgcc gaaagtagtg tctcggtcag tagtctgctc tgacactcgg gaccgggagg 121 aatatgacga cggcgagaag cccctccatg tttactactg tttgtgcggc cagatggtcc 181 tagtgctgga ctgccagtta gagaaattgc ccatgaggcc ccgggaccgg tcccgtgtga 241 ttgatgctgc caaacatgcc cataagtttt gtaacacaga agatgaggag actatgtatc 301 tgcggagacc tgaaggcatt gaacgacagt aCAGGAAGAA ATGTGCAAAG TGTGGACTGC 361 CGCTCTTCTA CCAATCCCAG CCAAAGAATG CTCCTGTTAC CTTCATTGTG GATGGAGCAG 421 TAGTCAAGTT TGGCCAGGGT TTTGGGAAAA CGAACATATA TACTCAGAAA CAAGAGCCTC 481 CTAAGAAGGT GATGATGACC AAACGGACCA AAGACATGGG CAAGTTCAGT TCTGTCACCG 541 TGTCTACCAT TGATGAAGAG GAAGAGGAGA TTGAGGCTAG GGAAGTTGCT GACTCATATG 601 CACAGAATGC CAAAGTGATT GAAAAACAGC TGGAGCGCAA AGGCATGAGC AAGAGGCGAC 661 TGCAAGAGCT GGCTGAATTG GAAGCCAAGA AAGCGAAAAT GAAGGGGACC TTGATTGACA 721 ACCAGTTCAA ATACCCAACT TTCTTGTACA AAGTGGTTGA TATCGGTAAG CCTATCCCTA 781 ACCCTCTCCT CGGTCTCGAT TCTACGTAGT AATGAACTAG TCCGTAACTT GAAAGTATTT 841 CGATTTCTTG GCTTTATATA TCTTGTGGAA AGGACGAGGC GCCCTCACTT TTCACATATC 901 AACGCGTTAA GTCgacaatc aacctctgga ttacaaaatt tgtgaaagat t