Construct: ORF TRCN0000480996
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF016890.1_s317c1
- Derived from:
- ccsbBroadEn_05497
- DNA Barcode:
- TCGCGGAGACTCACCCAATCCCGC
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- ACTBL2 (345651)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000480996
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 345651 | ACTBL2 | actin beta like 2 | NM_001017992.4 | 100% | 100% | |
| 2 | human | 60 | ACTB | actin beta | NM_001101.5 | 81.6% | 91.2% | (many diffs) |
| 3 | human | 71 | ACTG1 | actin gamma 1 | NM_001199954.2 | 80.9% | 90.9% | (many diffs) |
| 4 | human | 71 | ACTG1 | actin gamma 1 | NM_001614.5 | 80.9% | 90.9% | (many diffs) |
| 5 | human | 72 | ACTG2 | actin gamma 2, smooth muscle | NM_001615.4 | 80.4% | 87.7% | (many diffs) |
| 6 | human | 72 | ACTG2 | actin gamma 2, smooth muscle | NM_001199893.2 | 71.4% | 77.3% | (many diffs) |
| 7 | mouse | 238880 | Actbl2 | actin, beta-like 2 | NM_175497.3 | 89.4% | 97% | (many diffs) |
| 8 | mouse | 11461 | Actb | actin, beta | NM_007393.5 | 81.4% | 91.2% | (many diffs) |
| 9 | mouse | 11465 | Actg1 | actin, gamma, cytoplasmic 1 | NM_009609.3 | 80.7% | 90.9% | (many diffs) |
| 10 | mouse | 11459 | Acta1 | actin, alpha 1, skeletal mu... | NM_001272041.1 | 79.2% | 87% | (many diffs) |
| 11 | mouse | 11459 | Acta1 | actin, alpha 1, skeletal mu... | NM_009606.3 | 79.2% | 87% | (many diffs) |
| 12 | mouse | 11465 | Actg1 | actin, gamma, cytoplasmic 1 | NM_001313923.1 | 54.5% | 62.7% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 1197
- ORF length:
- 1128
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcaccat gactgacaat gagctgtctg ccttggtagt ggataatggg tcagggatgt 121 gcaaggcagg ctttggtggt gacgatgccc cccgggctgt gttcccctcc atgatagggc 181 gtcctcgaca ccagggcgtt atggtaggca tgggccagaa ggactgctac gtgggagatg 241 aggctcagag caagagaggc gtcctgaccc tgaagtatcc tatcgagcat ggagtggtca 301 ccaactggga cgatatggaa aagatctggt accacacatt ctacaatgag ctccgtgtgg 361 caccagatga gcatcccatc ctcctcaccg aggcacccct gaaccccaag atcaaccggg 421 aaaagatgac tcagatcatg ttcgaggctt tcaacacacc agccatgtat gtcgccatcc 481 aggctgtgct gtccctctat gcctcaggac ggaccacagg catcgtgatg gattctgggg 541 atggggtcac tcacatcgtg cccatctatg aaggttatgc cctgcctcat gccattctac 601 gcctggatct ggcagggaga gacctgactg attacctcat gaagatcctg acagagcgag 661 gctataactt caccaccact gctgagcggg agattgtgcg agatgtcaaa gagaagctgt 721 gctacgtagc cctggacttt gagcaggaga tggtcagggc agccgcatcc tcctcaccgg 781 aacggagcta tgaacttccT GATGGGCAGG TGATCACCAT TGGGAATGAA CGCTTCCGAT 841 GCCCTGAAGC CATTTTCCAG CCTTCCTTTC TGGGCATTGA GTCCAGTGGG ATCCATGAGA 901 CAACCTTCAA CTCTATCATG AAGTGCGATG TGGATATTCG CAAGGATCTC TATGCCAACA 961 CCGTGTTATC TGGAGGGAGC ACCATGTACC CAGGCATTGC TGACAGGATG CAGAAAGAAA 1021 TCATAACCCT GGCACCCAGC ACCATGAAAA TCAAGATCAT AGCTCCCCCA GAGCGGAAGT 1081 ATTCTGTTTG GATTGGGGGC TCCATCCTTG CCAGCCTGTC CACATTCCAG CAGATGTGGA 1141 TCAGTAAGCA GGAATATGAT GAGGCTGGTC CTCCTATCGT TCACAGAAAA TGTTTCTTGC 1201 CAACTTTCTT GTACAAAGTG GTTGATATCG GTAAGCCTAT CCCTAACCCT CTCCTCGGTC 1261 TCGATTCTAC GTAGTAATGA ACTAGTCCGT AACTTGAAAG TATTTCGATT TCTTGGCTTT 1321 ATATATCTTG TGGAAAGGAC GATCGCGGAG ACTCACCCAA TCCCGCACGC GTTAAGTCga 1381 caatcaacct ctggattaca aaatttgtga aagatt