Construct: ORF TRCN0000481220

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF017364.1_s317c1
Derived from:
ccsbBroadEn_01285
DNA Barcode:
TCTTTTCTGTTAAAGGACCACACA
Epitope Tag:
V5
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
PRKG1 (5592)

Vector Information:

Vector Backbone:
pLX_317
Pol II Cassette 1:
SV40-PuroR
Pol II Cassette 2:
EF1a-TRCN0000481220
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
V5

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 5592 PRKG1 protein kinase cGMP-depende... NM_006258.4 100% 100%
2 human 5592 PRKG1 protein kinase cGMP-depende... NM_001098512.3 91.8% 88.7% (many diffs)
3 human 5592 PRKG1 protein kinase cGMP-depende... XM_017016412.1 85.9% 85.1% (many diffs)
4 human 5592 PRKG1 protein kinase cGMP-depende... XM_017016413.1 85.2% 84.8% 0_1ins277;4_5insGACCCTGCCCTTCTACCC;8_9insAGCCCACA
5 human 5592 PRKG1 protein kinase cGMP-depende... XM_011539952.2 56.9% 54% (many diffs)
6 human 5592 PRKG1 protein kinase cGMP-depende... XM_024448078.1 41.2% 41.2% 0_1ins1209
7 mouse 19091 Prkg1 protein kinase, cGMP-depend... NM_011160.3 93.1% 99.5% (many diffs)
8 mouse 19091 Prkg1 protein kinase, cGMP-depend... NM_001013833.3 85.7% 88.3% (many diffs)
9 mouse 19091 Prkg1 protein kinase, cGMP-depend... XM_006526768.3 85.7% 88.3% (many diffs)
Download CSV

Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
69
ORF end:
2127
ORF length:
2058
Sequence:
1tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag
61ttggcaccat gggcaccttg cgggatttac agtacgcgct ccaggagaag atcgaggagc
121tgaggcagcg ggatgctctc atcgacgagc tggagctgga gttggatcag aaggacgaac
181tgatccagaa gctgcagaac gagctggaca agtaccgctc ggtgatccga ccagccaccc
241agcaggcgca gaagcagagc gcgagcacct tgcagggcga gccgcgcacc aagcggcagg
301cgatctccgc cgagcccacc gccttcgaca tccaggatct cagccatgtg accctgccct
361tctaccccaa gagcccacag tccaaggatc ttataaagga agctatcctt gacaatgact
421ttatgaagaa cttggagctg tcgcagatcc aggagattgt ggattgtatg tacccggtgg
481agtatggcaa ggacagttgc atcatcaaag aaggagacgt ggggtcactg gtgtatgtca
541tggaagatgg taaggttgaa gttacaaaag aaggtgtgaa gttgtgtacc atgggtccag
601gaaaagtgtt tggggaattg gctattcttt acaactgtac ccggacagcg accgtcaaga
661ctcttgtaaa tgtaaaactc tgggccattg atcgacaatg ttttcaaaca ataatgatga
721ggacaggact catcaagcat accgagtata tggaattttt aaaaagcgtt ccaacattcc
781agagccttcc tgaagagatc ctcagcaagc ttgctgatgt ccttgaagag acccactatg
841aaaatggaga atatattatc aggcaaggtg caagagggga caccttcttt atcatcagca
901aaggaacggt aaatgtcact cgtgaagact caccgagtga agacccagtc tttcttagaa
961ctttaggaaa aggagactgg tttggagaga aagccttgca gggggaagat gtgagaacag
1021caaacgtaat tgctgcagaa gctgtaacct gccttgtgat tgacagagac tcttttaaac
1081atttgattgg agggctggat gatgtttcta ataaagcata tgaagatgca gaagctaaag
1141caaaatatga agctgaagcg gctttcttcg ccaacctgaa gctgtctgat ttcaacatca
1201ttgataccct tggagttgga ggtttcggac gagtagaact ggtccagttg aaaagtgaag
1261aatccaaaac gtttgcaatg aagattctca agaaacgtca cattgtggac acaagacagc
1321aggagcacat ccgctcagag aagcagatca tgcagggggc tcattccgat ttcatagtga
1381gactgtacag aacatttaag gacagcaaat atttgtatat gttgatggaa gcttgtctag
1441gtggagagct ctggaccatt ctcagggata gaggttcgtt tgaagattct acaaccagat
1501tttacacagc atgtgtggta gaagcttttg cctatctgca ttccaaagga atcatttaca
1561gggacctcaa gccagaaaat ctcatcctag atcaccgagg ttatgccaaa ctggttgatt
1621ttggctttgc aaagaaaata ggatttGGAA AGAAAACATG GACTTTTTGT GGGACTCCAG
1681AGTATGTAGC CCCAGAGATC ATCCTGAACA AAGGCCATGA CATTTCAGCC GACTACTGGT
1741CACTGGGAAT CCTAATGTAT GAACTCCTGA CTGGCAGCCC ACCTTTCTCA GGCCCAGATC
1801CTATGAAAAC CTATAACATC ATATTGAGGG GGATTGACAT GATAGAATTT CCAAAGAAGA
1861TTGCCAAAAA TGCTGCTAAT TTAATTAAAA AACTATGCAG GGACAATCCA TCAGAAAGAT
1921TAGGGAATTT GAAAAATGGA GTAAAAGACA TTCAAAAGCA CAAATGGTTT GAGGGCTTTA
1981ACTGGGAAGG CTTAAGAAAA GGTACCTTGA CACCTCCTAT AATACCAAGT GTTGCATCAC
2041CCACAGACAC AAGTAATTTT GACAGTTTCC CTGAGGACAA CGATGAACCA CCACCTGATG
2101ACAACTCAGG ATGGGATATA GACTTCTTGC CAACTTTCTT GTACAAAGTG GTTGATATCG
2161GTAAGCCTAT CCCTAACCCT CTCCTCGGTC TCGATTCTAC GTAGTAATGA ACTAGTCCGT
2221AACTTGAAAG TATTTCGATT TCTTGGCTTT ATATATCTTG TGGAAAGGAC GATCTTTTCT
2281GTTAAAGGAC CACACAACGC GTTAAGTCga caatcaacct ctggattaca aaatttgtga
2341aagatt

Download FASTA (ORF) (Full)