Construct: ORF TRCN0000481220
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF017364.1_s317c1
- Derived from:
- ccsbBroadEn_01285
- DNA Barcode:
- TCTTTTCTGTTAAAGGACCACACA
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- PRKG1 (5592)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000481220
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 5592 | PRKG1 | protein kinase cGMP-depende... | NM_006258.4 | 100% | 100% | |
| 2 | human | 5592 | PRKG1 | protein kinase cGMP-depende... | NM_001098512.3 | 91.8% | 88.7% | (many diffs) |
| 3 | human | 5592 | PRKG1 | protein kinase cGMP-depende... | XM_017016412.1 | 85.9% | 85.1% | (many diffs) |
| 4 | human | 5592 | PRKG1 | protein kinase cGMP-depende... | XM_017016413.1 | 85.2% | 84.8% | 0_1ins277;4_5insGACCCTGCCCTTCTACCC;8_9insAGCCCACA |
| 5 | human | 5592 | PRKG1 | protein kinase cGMP-depende... | XM_011539952.2 | 56.9% | 54% | (many diffs) |
| 6 | human | 5592 | PRKG1 | protein kinase cGMP-depende... | XM_024448078.1 | 41.2% | 41.2% | 0_1ins1209 |
| 7 | mouse | 19091 | Prkg1 | protein kinase, cGMP-depend... | NM_011160.3 | 93.1% | 99.5% | (many diffs) |
| 8 | mouse | 19091 | Prkg1 | protein kinase, cGMP-depend... | NM_001013833.3 | 85.7% | 88.3% | (many diffs) |
| 9 | mouse | 19091 | Prkg1 | protein kinase, cGMP-depend... | XM_006526768.3 | 85.7% | 88.3% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 2127
- ORF length:
- 2058
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcaccat gggcaccttg cgggatttac agtacgcgct ccaggagaag atcgaggagc 121 tgaggcagcg ggatgctctc atcgacgagc tggagctgga gttggatcag aaggacgaac 181 tgatccagaa gctgcagaac gagctggaca agtaccgctc ggtgatccga ccagccaccc 241 agcaggcgca gaagcagagc gcgagcacct tgcagggcga gccgcgcacc aagcggcagg 301 cgatctccgc cgagcccacc gccttcgaca tccaggatct cagccatgtg accctgccct 361 tctaccccaa gagcccacag tccaaggatc ttataaagga agctatcctt gacaatgact 421 ttatgaagaa cttggagctg tcgcagatcc aggagattgt ggattgtatg tacccggtgg 481 agtatggcaa ggacagttgc atcatcaaag aaggagacgt ggggtcactg gtgtatgtca 541 tggaagatgg taaggttgaa gttacaaaag aaggtgtgaa gttgtgtacc atgggtccag 601 gaaaagtgtt tggggaattg gctattcttt acaactgtac ccggacagcg accgtcaaga 661 ctcttgtaaa tgtaaaactc tgggccattg atcgacaatg ttttcaaaca ataatgatga 721 ggacaggact catcaagcat accgagtata tggaattttt aaaaagcgtt ccaacattcc 781 agagccttcc tgaagagatc ctcagcaagc ttgctgatgt ccttgaagag acccactatg 841 aaaatggaga atatattatc aggcaaggtg caagagggga caccttcttt atcatcagca 901 aaggaacggt aaatgtcact cgtgaagact caccgagtga agacccagtc tttcttagaa 961 ctttaggaaa aggagactgg tttggagaga aagccttgca gggggaagat gtgagaacag 1021 caaacgtaat tgctgcagaa gctgtaacct gccttgtgat tgacagagac tcttttaaac 1081 atttgattgg agggctggat gatgtttcta ataaagcata tgaagatgca gaagctaaag 1141 caaaatatga agctgaagcg gctttcttcg ccaacctgaa gctgtctgat ttcaacatca 1201 ttgataccct tggagttgga ggtttcggac gagtagaact ggtccagttg aaaagtgaag 1261 aatccaaaac gtttgcaatg aagattctca agaaacgtca cattgtggac acaagacagc 1321 aggagcacat ccgctcagag aagcagatca tgcagggggc tcattccgat ttcatagtga 1381 gactgtacag aacatttaag gacagcaaat atttgtatat gttgatggaa gcttgtctag 1441 gtggagagct ctggaccatt ctcagggata gaggttcgtt tgaagattct acaaccagat 1501 tttacacagc atgtgtggta gaagcttttg cctatctgca ttccaaagga atcatttaca 1561 gggacctcaa gccagaaaat ctcatcctag atcaccgagg ttatgccaaa ctggttgatt 1621 ttggctttgc aaagaaaata ggatttGGAA AGAAAACATG GACTTTTTGT GGGACTCCAG 1681 AGTATGTAGC CCCAGAGATC ATCCTGAACA AAGGCCATGA CATTTCAGCC GACTACTGGT 1741 CACTGGGAAT CCTAATGTAT GAACTCCTGA CTGGCAGCCC ACCTTTCTCA GGCCCAGATC 1801 CTATGAAAAC CTATAACATC ATATTGAGGG GGATTGACAT GATAGAATTT CCAAAGAAGA 1861 TTGCCAAAAA TGCTGCTAAT TTAATTAAAA AACTATGCAG GGACAATCCA TCAGAAAGAT 1921 TAGGGAATTT GAAAAATGGA GTAAAAGACA TTCAAAAGCA CAAATGGTTT GAGGGCTTTA 1981 ACTGGGAAGG CTTAAGAAAA GGTACCTTGA CACCTCCTAT AATACCAAGT GTTGCATCAC 2041 CCACAGACAC AAGTAATTTT GACAGTTTCC CTGAGGACAA CGATGAACCA CCACCTGATG 2101 ACAACTCAGG ATGGGATATA GACTTCTTGC CAACTTTCTT GTACAAAGTG GTTGATATCG 2161 GTAAGCCTAT CCCTAACCCT CTCCTCGGTC TCGATTCTAC GTAGTAATGA ACTAGTCCGT 2221 AACTTGAAAG TATTTCGATT TCTTGGCTTT ATATATCTTG TGGAAAGGAC GATCTTTTCT 2281 GTTAAAGGAC CACACAACGC GTTAAGTCga caatcaacct ctggattaca aaatttgtga 2341 aagatt