Transcript: Human XM_011539952.2

PREDICTED: Homo sapiens protein kinase cGMP-dependent 1 (PRKG1), transcript variant X3, mRNA.

Source:
NCBI, updated 2019-09-08
Taxon:
Homo sapiens (human)
Gene:
PRKG1 (5592)
Length:
2879
CDS:
462..1682

Additional Resources:

NCBI RefSeq record:
XM_011539952.2
NBCI Gene record:
PRKG1 (5592)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

Clone ID Target Seq Vector PAM Seq Cut Position Cut % of CDS Length Exon On Target Score[?] Other Matching Genes Orig. Target Gene[?] Notes Addgene[?]
1 BRDN0001147277 TGTGGATTGTATGTACCCGG pXPR_003 TGG 406 33% 2 0.8278 PRKG1 PRKG1 77006
2 BRDN0001146382 CTTACTCTCTGTCAATCACA pXPR_003 AGG 989 81% 8 0.2073 PRKG1 PRKG1 77004
3 BRDN0001147664 GGGTCCAGGAAAAGTGTTTG pXPR_003 GGG 541 44% 3 -0.3815 PRKG1 PRKG1 77005
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shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Human XM_011539952.2, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Human Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000355918 TCATCAAGCATACCGAGTATA pLKO_005 1123 CDS 100% 13.200 18.480 N PRKG1 n/a
2 TRCN0000000997 GCTGGATGATGTTTCTAATAA pLKO.1 1487 CDS 100% 15.000 10.500 N PRKG1 n/a
3 TRCN0000194729 CCAGTCTTTCTTAGAACTTTA pLKO.1 1338 CDS 100% 13.200 9.240 N PRKG1 n/a
4 TRCN0000256886 GACCGTCAAGACTCTTGTAAA pLKO_005 1043 CDS 100% 13.200 9.240 N PRKG1 n/a
5 TRCN0000010033 CATCAGCAAAGGAACGGTAAA pLKO.1 1286 CDS 100% 10.800 7.560 N PRKG1 n/a
6 TRCN0000055432 CATCAGCAAAGGAACGGTAAA pLKO.1 1286 CDS 100% 10.800 7.560 N PRKG1 n/a
7 TRCN0000022782 CGACCGTCAAGACTCTTGTAA pLKO.1 1042 CDS 100% 5.625 3.938 N Prkg1 n/a
8 TRCN0000010032 GGACAGGACTCATCAAGCATA pLKO.1 1114 CDS 100% 4.950 3.465 N PRKG1 n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript XM_011539952.2, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_14797 pDONR223 73.4% 57.4% 81.8% None (many diffs) n/a
2 ccsbBroad304_14797 pLX_304 0% 57.4% 81.8% V5 (not translated due to prior stop codon) (many diffs) n/a
3 TRCN0000470987 CCGTGGCAATCTGGAAATAATCAC pLX_317 18.4% 57.4% 81.8% V5 (not translated due to prior stop codon) (many diffs) n/a
4 ccsbBroadEn_01285 pDONR223 100% 56.9% 54% None (many diffs) n/a
5 ccsbBroad304_01285 pLX_304 0% 56.9% 54% V5 (many diffs) n/a
6 TRCN0000481220 TCTTTTCTGTTAAAGGACCACACA pLX_317 23.5% 56.9% 54% V5 (many diffs) n/a
7 TRCN0000488905 TCATTGATAGAAAGAGTCCTGCAC pLX_317 17.1% 56.9% 54% V5 (not translated due to prior stop codon) (many diffs) n/a
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