Transcript: Human XM_024448078.1

PREDICTED: Homo sapiens protein kinase cGMP-dependent 1 (PRKG1), transcript variant X4, mRNA.

Source:
NCBI, updated 2019-09-08
Taxon:
Homo sapiens (human)
Gene:
PRKG1 (5592)
Length:
6127
CDS:
826..1677

Additional Resources:

NCBI RefSeq record:
XM_024448078.1
NBCI Gene record:
PRKG1 (5592)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Human XM_024448078.1, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Human Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000256885 ACCTCTCATAGTAGGTATATA pLKO_005 2332 3UTR 100% 15.000 21.000 N PRKG1 n/a
2 TRCN0000355854 ACCTTGACACCTCCTATAATA pLKO_005 1552 CDS 100% 15.000 21.000 N PRKG1 n/a
3 TRCN0000355919 ATTACTGCAGGCCGGTGTATA pLKO_005 2132 3UTR 100% 13.200 18.480 N PRKG1 n/a
4 TRCN0000000996 GTCCATATACTTGTCTAACTA pLKO.1 3053 3UTR 100% 5.625 7.875 N PRKG1 n/a
5 TRCN0000010031 GTACCTTGACACCTCCTATAA pLKO.1 1550 CDS 100% 0.000 0.000 N PRKG1 n/a
6 TRCN0000010026 GGCTCATTCCGATTTCATAGT pLKO.1 906 CDS 100% 4.950 3.960 N PRKG1 n/a
7 TRCN0000010030 TCCTAATGTATGAACTCCTGA pLKO.1 1298 CDS 100% 2.640 2.112 N PRKG1 n/a
8 TRCN0000194810 CCGGTGTATATACCATACAAA pLKO.1 2143 3UTR 100% 0.563 0.450 N PRKG1 n/a
9 TRCN0000360701 CCTTGCTTTGCTCTGATTATA pLKO_005 1955 3UTR 100% 15.000 10.500 N Prkg1 n/a
10 TRCN0000000997 GCTGGATGATGTTTCTAATAA pLKO.1 642 5UTR 100% 15.000 10.500 N PRKG1 n/a
11 TRCN0000267652 ACAACTCAGGATGGGATATAG pLKO_005 1649 CDS 100% 13.200 9.240 N PRKG1 n/a
12 TRCN0000194729 CCAGTCTTTCTTAGAACTTTA pLKO.1 493 5UTR 100% 13.200 9.240 N PRKG1 n/a
13 TRCN0000256883 TGCATTCCAAAGGAATCATTT pLKO_005 1085 CDS 100% 13.200 9.240 N PRKG1 n/a
14 TRCN0000360727 TGCATTCCAAAGGAATCATTT pLKO_005 1085 CDS 100% 13.200 9.240 N Prkg1 n/a
15 TRCN0000256884 TTATGCCAAACTGGTTGATTT pLKO_005 1149 CDS 100% 13.200 9.240 N PRKG1 n/a
16 TRCN0000010033 CATCAGCAAAGGAACGGTAAA pLKO.1 441 5UTR 100% 10.800 7.560 N PRKG1 n/a
17 TRCN0000055432 CATCAGCAAAGGAACGGTAAA pLKO.1 441 5UTR 100% 10.800 7.560 N PRKG1 n/a
18 TRCN0000022781 CCAGAGATCATCCTGAACAAA pLKO.1 1240 CDS 100% 5.625 3.938 N Prkg1 n/a
19 TRCN0000195088 CCTCCTATAATACCAAGTGTT pLKO.1 1561 CDS 100% 4.950 3.465 N PRKG1 n/a
20 TRCN0000196847 GCTGTCTGATTTCAACATCAT pLKO.1 729 5UTR 100% 4.950 3.465 N PRKG1 n/a
21 TRCN0000000998 CAGAACATTTAAGGACAGCAA pLKO.1 936 CDS 100% 2.640 1.848 N PRKG1 n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript XM_024448078.1, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_01285 pDONR223 100% 41.2% 41.2% None 0_1ins1209 n/a
2 ccsbBroad304_01285 pLX_304 0% 41.2% 41.2% V5 0_1ins1209 n/a
3 TRCN0000481220 TCTTTTCTGTTAAAGGACCACACA pLX_317 23.5% 41.2% 41.2% V5 0_1ins1209 n/a
4 TRCN0000488905 TCATTGATAGAAAGAGTCCTGCAC pLX_317 17.1% 41.2% 41.2% V5 (not translated due to prior stop codon) 0_1ins1209 n/a
5 ccsbBroadEn_14797 pDONR223 73.4% 41.1% 4.8% None (many diffs) n/a
6 ccsbBroad304_14797 pLX_304 0% 41.1% 4.8% V5 (not translated due to prior stop codon) (many diffs) n/a
7 TRCN0000470987 CCGTGGCAATCTGGAAATAATCAC pLX_317 18.4% 41.1% 4.8% V5 (not translated due to prior stop codon) (many diffs) n/a
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