Transcript: Human NM_006258.4

Homo sapiens protein kinase cGMP-dependent 1 (PRKG1), transcript variant 2, mRNA.

Source:
NCBI, updated 2019-07-31
Taxon:
Homo sapiens (human)
Gene:
PRKG1 (5592)
Length:
6615
CDS:
105..2165

Additional Resources:

NCBI RefSeq record:
NM_006258.4
NBCI Gene record:
PRKG1 (5592)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

Clone ID Target Seq Vector PAM Seq Cut Position Cut % of CDS Length Exon On Target Score[?] Other Matching Genes Orig. Target Gene[?] Notes Addgene[?]
1 BRDN0001147277 TGTGGATTGTATGTACCCGG pXPR_003 TGG 406 20% 2 0.8113 PRKG1 PRKG1 77006
2 BRDN0001146382 CTTACTCTCTGTCAATCACA pXPR_003 AGG 989 48% 8 0.1777 PRKG1 PRKG1 77004
3 BRDN0001147664 GGGTCCAGGAAAAGTGTTTG pXPR_003 GGG 541 26% 3 -0.3895 PRKG1 PRKG1 77005
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shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Human NM_006258.4, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Human Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000256885 ACCTCTCATAGTAGGTATATA pLKO_005 2820 3UTR 100% 15.000 21.000 N PRKG1 n/a
2 TRCN0000355854 ACCTTGACACCTCCTATAATA pLKO_005 2040 CDS 100% 15.000 21.000 N PRKG1 n/a
3 TRCN0000355919 ATTACTGCAGGCCGGTGTATA pLKO_005 2620 3UTR 100% 13.200 18.480 N PRKG1 n/a
4 TRCN0000355918 TCATCAAGCATACCGAGTATA pLKO_005 766 CDS 100% 13.200 18.480 N PRKG1 n/a
5 TRCN0000000996 GTCCATATACTTGTCTAACTA pLKO.1 3541 3UTR 100% 5.625 7.875 N PRKG1 n/a
6 TRCN0000010031 GTACCTTGACACCTCCTATAA pLKO.1 2038 CDS 100% 0.000 0.000 N PRKG1 n/a
7 TRCN0000010026 GGCTCATTCCGATTTCATAGT pLKO.1 1394 CDS 100% 4.950 3.960 N PRKG1 n/a
8 TRCN0000010030 TCCTAATGTATGAACTCCTGA pLKO.1 1786 CDS 100% 2.640 2.112 N PRKG1 n/a
9 TRCN0000194810 CCGGTGTATATACCATACAAA pLKO.1 2631 3UTR 100% 0.563 0.450 N PRKG1 n/a
10 TRCN0000360701 CCTTGCTTTGCTCTGATTATA pLKO_005 2443 3UTR 100% 15.000 10.500 N Prkg1 n/a
11 TRCN0000000997 GCTGGATGATGTTTCTAATAA pLKO.1 1130 CDS 100% 15.000 10.500 N PRKG1 n/a
12 TRCN0000267652 ACAACTCAGGATGGGATATAG pLKO_005 2137 CDS 100% 13.200 9.240 N PRKG1 n/a
13 TRCN0000194729 CCAGTCTTTCTTAGAACTTTA pLKO.1 981 CDS 100% 13.200 9.240 N PRKG1 n/a
14 TRCN0000256886 GACCGTCAAGACTCTTGTAAA pLKO_005 686 CDS 100% 13.200 9.240 N PRKG1 n/a
15 TRCN0000256883 TGCATTCCAAAGGAATCATTT pLKO_005 1573 CDS 100% 13.200 9.240 N PRKG1 n/a
16 TRCN0000360727 TGCATTCCAAAGGAATCATTT pLKO_005 1573 CDS 100% 13.200 9.240 N Prkg1 n/a
17 TRCN0000256884 TTATGCCAAACTGGTTGATTT pLKO_005 1637 CDS 100% 13.200 9.240 N PRKG1 n/a
18 TRCN0000010033 CATCAGCAAAGGAACGGTAAA pLKO.1 929 CDS 100% 10.800 7.560 N PRKG1 n/a
19 TRCN0000055432 CATCAGCAAAGGAACGGTAAA pLKO.1 929 CDS 100% 10.800 7.560 N PRKG1 n/a
20 TRCN0000022781 CCAGAGATCATCCTGAACAAA pLKO.1 1728 CDS 100% 5.625 3.938 N Prkg1 n/a
21 TRCN0000022782 CGACCGTCAAGACTCTTGTAA pLKO.1 685 CDS 100% 5.625 3.938 N Prkg1 n/a
22 TRCN0000195088 CCTCCTATAATACCAAGTGTT pLKO.1 2049 CDS 100% 4.950 3.465 N PRKG1 n/a
23 TRCN0000196847 GCTGTCTGATTTCAACATCAT pLKO.1 1217 CDS 100% 4.950 3.465 N PRKG1 n/a
24 TRCN0000010032 GGACAGGACTCATCAAGCATA pLKO.1 757 CDS 100% 4.950 3.465 N PRKG1 n/a
25 TRCN0000000998 CAGAACATTTAAGGACAGCAA pLKO.1 1424 CDS 100% 2.640 1.848 N PRKG1 n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript NM_006258.4, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_01285 pDONR223 100% 100% 100% None n/a
2 ccsbBroad304_01285 pLX_304 0% 100% 100% V5 n/a
3 TRCN0000481220 TCTTTTCTGTTAAAGGACCACACA pLX_317 23.5% 100% 100% V5 n/a
4 TRCN0000488905 TCATTGATAGAAAGAGTCCTGCAC pLX_317 17.1% 100% 100% V5 (not translated due to prior stop codon) n/a
5 ccsbBroadEn_14797 pDONR223 73.4% 99.8% 63.4% None (many diffs) n/a
6 ccsbBroad304_14797 pLX_304 0% 99.8% 63.4% V5 (not translated due to prior stop codon) (many diffs) n/a
7 TRCN0000470987 CCGTGGCAATCTGGAAATAATCAC pLX_317 18.4% 99.8% 63.4% V5 (not translated due to prior stop codon) (many diffs) n/a
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