Construct: ORF TRCN0000481296
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF000335.2_s317c1
- Derived from:
- ccsbBroadEn_01249
- DNA Barcode:
- GTATAACGCCGGAGATCCACGACA
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- PPARG (5468)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000481296
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 5468 | PPARG | peroxisome proliferator act... | NM_001354666.2 | 100% | 100% | |
| 2 | human | 5468 | PPARG | peroxisome proliferator act... | NM_001354667.2 | 100% | 100% | |
| 3 | human | 5468 | PPARG | peroxisome proliferator act... | NM_001374263.1 | 100% | 100% | |
| 4 | human | 5468 | PPARG | peroxisome proliferator act... | NM_001374264.1 | 100% | 100% | |
| 5 | human | 5468 | PPARG | peroxisome proliferator act... | NM_005037.6 | 100% | 100% | |
| 6 | human | 5468 | PPARG | peroxisome proliferator act... | NM_138711.4 | 100% | 100% | |
| 7 | human | 5468 | PPARG | peroxisome proliferator act... | NM_138712.4 | 100% | 100% | |
| 8 | human | 5468 | PPARG | peroxisome proliferator act... | NM_015869.5 | 94.4% | 94.4% | 1_84del |
| 9 | human | 5468 | PPARG | peroxisome proliferator act... | NM_001354669.2 | 55.7% | 47.6% | 0_1ins433;102_103ins200 |
| 10 | human | 5468 | PPARG | peroxisome proliferator act... | NM_001354670.2 | 54.6% | 52% | (many diffs) |
| 11 | human | 5468 | PPARG | peroxisome proliferator act... | NM_001354668.2 | 51.6% | 49.2% | (many diffs) |
| 12 | human | 5468 | PPARG | peroxisome proliferator act... | NM_001330615.2 | 51.6% | 51.3% | (many diffs) |
| 13 | human | 5468 | PPARG | peroxisome proliferator act... | NM_001374261.1 | 51.6% | 51.3% | (many diffs) |
| 14 | human | 5468 | PPARG | peroxisome proliferator act... | NM_001374262.1 | 51.6% | 51.3% | (many diffs) |
| 15 | human | 5468 | PPARG | peroxisome proliferator act... | NM_001374265.1 | 48.8% | 48.5% | (many diffs) |
| 16 | human | 5468 | PPARG | peroxisome proliferator act... | NM_001374266.1 | 46.3% | 46.3% | (many diffs) |
| 17 | mouse | 19016 | Pparg | peroxisome proliferator act... | NM_001127330.2 | 90.2% | 97.9% | (many diffs) |
| 18 | mouse | 19016 | Pparg | peroxisome proliferator act... | NM_001308352.1 | 90.2% | 97.9% | (many diffs) |
| 19 | mouse | 19016 | Pparg | peroxisome proliferator act... | NM_001308354.1 | 90.2% | 97.9% | (many diffs) |
| 20 | mouse | 19016 | Pparg | peroxisome proliferator act... | XM_006505743.3 | 90.2% | 97.9% | (many diffs) |
| 21 | mouse | 19016 | Pparg | peroxisome proliferator act... | XM_011241252.1 | 90.2% | 97.9% | (many diffs) |
| 22 | mouse | 19016 | Pparg | peroxisome proliferator act... | XM_017321455.1 | 90.2% | 97.9% | (many diffs) |
| 23 | mouse | 19016 | Pparg | peroxisome proliferator act... | XM_006505737.3 | 86.4% | 93.6% | (many diffs) |
| 24 | mouse | 19016 | Pparg | peroxisome proliferator act... | XM_006505738.3 | 86.4% | 93.6% | (many diffs) |
| 25 | mouse | 19016 | Pparg | peroxisome proliferator act... | XM_006505739.2 | 86.4% | 93.6% | (many diffs) |
| 26 | mouse | 19016 | Pparg | peroxisome proliferator act... | NM_011146.3 | 85.6% | 92.6% | (many diffs) |
| 27 | mouse | 19016 | Pparg | peroxisome proliferator act... | XR_001785108.1 | 61.7% | (many diffs) | |
| 28 | mouse | 19016 | Pparg | peroxisome proliferator act... | XM_017321456.1 | 55.8% | 60.5% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1497
- ORF length:
- 1431
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgac catggttgac acagagatgc cattctggcc caccaacttt gggatcagct 121 ccgtggatct ctccgtaatg gaagaccact cccactcctt tgatatcaag cccttcacta 181 ctgttgactt ctccagcatt tctactccac attacgaaga cattccattc acaagaacag 241 atccagtggt tgcagattac aagtatgacc tgaaacttca agagtaccaa agtgcaatca 301 aagtggagcc tgcatctcca ccttattatt ctgagaagac tcagctctac aataagcctc 361 atgaagagcc ttccaactcc ctcatggcaa ttgaatgtcg tgtctgtgga gataaagctt 421 ctggatttca ctatggagtt catgcttgtg aaggatgcaa gggtttcttc cggagaacaa 481 tcagattgaa gcttatctat gacagatgtg atcttaactg tcggatccac aaaaaaagta 541 gaaataaatg tcagtactgt cggtttcaga aatgccttgc agtggggatg tctcataatg 601 ccatcaggtt tgggcggatg ccacaggccg agaaggagaa gctgttggcg gagatctcca 661 gtgatatcga ccagctgaat ccagagtccg ctgacctccg ggccctggca aaacatttgt 721 atgactcata cataaagtcc ttcccgctga ccaaagcaaa ggcgagggcg atcttgacag 781 gaaagacaac agacaaatca ccattcgtta tctatgacat gaattcctta atgatgggag 841 aagataaaat caagttcaaa cacatcaccc ccctgcagga gcagagcaaa gaggtggcca 901 tccgcatctt tcagggctgc cagtttcgct ccgtggaggc tgtgcaggag atcacagagt 961 atgccaaaag cattcctggt tttgtaaatc ttgacttgaa cgaccaagta actctcctca 1021 aatatggagt ccacgagatc atttacacaa tgctggcctc cttgatgaat aaagatggGG 1081 TTCTCATATC CGAGGGCCAA GGCTTCATGA CAAGGGAGTT TCTAAAGAGC CTGCGAAAGC 1141 CTTTTGGTGA CTTTATGGAG CCCAAGTTTG AGTTTGCTGT GAAGTTCAAT GCACTGGAAT 1201 TAGATGACAG CGACTTGGCA ATATTTATTG CTGTCATTAT TCTCAGTGGA GACCGCCCAG 1261 GTTTGCTGAA TGTGAAGCCC ATTGAAGACA TTCAAGACAA CCTGCTACAA GCCCTGGAGC 1321 TCCAGCTGAA GCTGAACCAC CCTGAGTCCT CACAGCTGTT TGCCAAGCTG CTCCAGAAAA 1381 TGACAGACCT CAGACAGATT GTCACGGAAC ACGTGCAGCT ACTGCAGGTG ATCAAGAAGA 1441 CGGAGACAGA CATGAGTCTT CACCCGCTCC TGCAGGAGAT CTACAAGGAC TTGTACTACC 1501 CAACTTTCTT GTACAAAGTG GTTGATATCG GTAAGCCTAT CCCTAACCCT CTCCTCGGTC 1561 TCGATTCTAC GTAGTAATGA ACTAGTCCGT AACTTGAAAG TATTTCGATT TCTTGGCTTT 1621 ATATATCTTG TGGAAAGGAC GAGTATAACG CCGGAGATCC ACGACAACGC GTTAAGTCga 1681 caatcaacct ctggattaca aaatttgtga aagatt