Construct: ORF TRCN0000481299

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF015462.3_s317c1
Derived from:
ccsbBroadEn_06727
DNA Barcode:
CCCACTCTGTAACCGTTGCGGCCC
Epitope Tag:
V5
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
PIK3CG (5294)

Vector Information:

Vector Backbone:
pLX_317
Pol II Cassette 1:
SV40-PuroR
Pol II Cassette 2:
EF1a-TRCN0000481299
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
V5

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 5294 PIK3CG phosphatidylinositol-4,5-bi... NM_001282426.2 99.8% 99.9% (many diffs)
2 human 5294 PIK3CG phosphatidylinositol-4,5-bi... NM_001282427.2 99.8% 99.9% (many diffs)
3 human 5294 PIK3CG phosphatidylinositol-4,5-bi... NM_002649.3 99.8% 99.9% (many diffs)
4 human 5294 PIK3CG phosphatidylinositol-4,5-bi... XM_005250443.3 99.8% 99.9% (many diffs)
5 human 5294 PIK3CG phosphatidylinositol-4,5-bi... XM_017012328.1 92.6% 91.9% (many diffs)
6 human 5294 PIK3CG phosphatidylinositol-4,5-bi... XM_011516316.1 91.5% 91.5% (many diffs)
7 human 5294 PIK3CG phosphatidylinositol-4,5-bi... XM_011516317.1 79.8% 79.4% (many diffs)
8 mouse 30955 Pik3cg phosphatidylinositol-4,5-bi... NM_001146200.1 87% 94% (many diffs)
9 mouse 30955 Pik3cg phosphatidylinositol-4,5-bi... NM_001146201.1 87% 94% (many diffs)
10 mouse 30955 Pik3cg phosphatidylinositol-4,5-bi... NM_020272.2 87% 94% (many diffs)
11 mouse 30955 Pik3cg phosphatidylinositol-4,5-bi... XM_006515112.3 87% 94% (many diffs)
12 mouse 30955 Pik3cg phosphatidylinositol-4,5-bi... XM_006515113.3 81.4% 86.4% (many diffs)
13 mouse 30955 Pik3cg phosphatidylinositol-4,5-bi... XR_872416.1 55.4% (many diffs)
14 mouse 30955 Pik3cg phosphatidylinositol-4,5-bi... XR_001780451.1 53.3% (many diffs)
Download CSV

Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
66
ORF end:
3372
ORF length:
3306
Sequence:
1tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag
61ttggcatggg gctggagaac tataaacagc ccgtggtgct gagagaggac aactgccgaa
121ggcgccggag gatgaagccg cgcagtgctg cggccagcct gtcctccatg gagctcatcc
181ccatcgagtt cgtgctgccc accagccagc gcaaatgcaa gagccccgaa acggcgctgc
241tgcacgtggc cggccacggc aacgtggagc agatgaaggc ccaggtgtgg ctgcgagcgc
301tggagaccag cgtggcggcg gacttctacc accggctggg accgcatcac ttcctcctgc
361tctatcagaa gaaggggcag tggtacgaga tctacgacaa gtaccaggtg gtgcagactc
421tggactgcct gcgctactgg aaggccacgc accggagccc gggccagatc cacctggtgc
481agcggcaccc gccctccgag gagtcccaag ccttccagcg gcagctcacg gcgctgattg
541gctatgacgt cactgacgtc agcaacgtgc acgacgatga gctggagttc acgcgccgtg
601gcttggtgac cccgcgcatg gcggaggtgg ccagccgcga ccccaagctc tacgccatgc
661acccgtgggt gacgtccaag cccctcccgg agtacctgtg gaagaagatt gccaacaact
721gcatcttcat cgtcattcac cgcagcacca ccagccagac cattaaggtc tcacccgacg
781acacccccgg cgccatcctg cagagcttct tcaccaagat ggccaagaag aaatctctga
841tggatattcc cgaaagccaa agcgaacagg attttgtgct gcgcgtctgt ggccgggatg
901agtacctggt gggcgaaacg cccatcaaaa acttccagtg ggtgaggcac tgcctcaaga
961acggagaaga gattcacgtg gtactggaca cgcctccaga cccggcccta gacgaggtga
1021ggaaggaaga gtggccgctg gtggacgact gcacgggagt caccggctac catgagcagc
1081ttaccatcca cggcaaggac cacgagagtg tgttcaccgt gtccctgtgg gactgcgacc
1141gcaagttcag ggtcaagatc agaggcattg atatccccgt cctgcctcgg aacaccgacc
1201tcacagtttt tgtagaggca aacatccagc atgggcaaca agtcctttgc caaaggagaa
1261ccagccccaa acccttcaca gaggaggtgc tgtggaatgt gtggcttgag ttcagtatca
1321aaatcaaaga cttgcccaaa ggggctctac tgaacctcca gatctactgc ggtaaagctc
1381cagcactgtc cagcaaggcc tctgcagagt cccccagttc tgagtccaag ggcaaagttc
1441agcttctcta ttatgtgaac ctgctgctga tagaccaccg tttcctcctg cgccgtggag
1501aatacgtcct ccacatgtgg cagatatctg ggaagggaga agaccaagga agcttcaatg
1561ctgacaaact cacgtctgca actaacccag acaaggagaa ctcaatgtcc atctccattc
1621ttctggacaa ttactgccac ccgatagccc tgcctaagca tcagcccacc cctgacccgg
1681aaggggaccg ggttcgagca gaaatgccca accagcttcg caagcaattg gaggcgatca
1741tagccactga tccacttaac cctctcacag cagaggacaa agaattgctc tggcatttta
1801gatacgaaag ccttaagcac ccaaaagcat atcctaagct atttagttca gtgaaatggg
1861gacagcaaga aattgtggcc aaaacatacc aattgttggc cagaagggaa gtctgggatc
1921aaagtgcttt ggatgttggg ttaacaatgc agctcctgga ctgcaacttc tcagatgaaa
1981atgtaagagc cattgcagtt cagaaactgg agagcttgga ggacgatgat gttctgcatt
2041accttctaca attggtccag gctgtgaaat ttgaaccata ccatgatagt gcccttgcca
2101gatttctgct gaagcgtggt ttaagaaaca aaagaattgg tcactttttg ttttggttct
2161tgagaagtga gatagcccag tccagacact atcagcagag gttcgctgtg attctggaag
2221cctatctgag gggctgtggc acagccatgc tgcacgactt tacccaacaa gtccaagtaa
2281tcgagatgtt acaaaaagtc acccttgata ttaaatcgct ctctgctgaa aagtatgacg
2341tcagttccca agttatttca caacttaaac aaaagcttga aaacctgcag aattctcaac
2401tccccgaaag ctttagagtt ccatatgatc ctggactgaa agcaggagcg ctggcaattg
2461aaaaatgtaa agtaatggcc tccaagaaaa aaccactatg gcttgagttt aaatgtgccg
2521atcctacagc cctatcaaat gaaacaattg gaattatctt taaacatggt gatgatctgc
2581gccaagacat gcttatttta cagattctac gaatcatgga gtctatttgg gagactgaat
2641ctttggatct atgcctcctg ccatatggtt gcatttcaac tggtgacaaa ataggaatga
2701tcgagattgt gaaagacgcc acgacaattg ccaaaattca gcaaagcaca gtgggcaaca
2761cgggagcatt taaagatgaa gtcctgaatc actggctcaa agaaaaatcc cctactgaag
2821aaaagtttca ggcagcagtg gagagatttg tttattcctg tgcaggctac tgtgtggcaa
2881cctttgttct tggaataggc gacagacaca atgacaatat tatgatcacc gagacaggaa
2941accTATTTCA TATTGACTTC GGGCACATTC TTGGGAATTA CAAAAGTTTC CTGGGCATTA
3001ATAAAGAGAG AGTGCCATTT GTGCTAACCC CTGACTTCCT CTTTGTGATG GGAACTTCTG
3061GAAAGAAGAC AAGCCCACAC TTCCAGAAAT TTCAGGACAT CTGTGTTAAG GCTTATCTAG
3121CCCTTCGTCA TCACACAAAC CTACTGATCA TCCTGTTCTC CATGATGCTG ATGACAGGAA
3181TGCCCCAGTT AACAAGCAAA GAAGACATTG AATATATCCG GGATGCCCTC ACAGTGGGGA
3241AAAATGAGGA GGATGCTAAA AAGTATTTTC TTGATCAGAT CGAAGTTTGC AGAGACAAAG
3301GATGGACTGT GCAGTTTAAT TGGTTTCTAC ATCTTGTTCT TGGCATCAAA CAAGGAGAGA
3361AACATTCAGC CTACCCAACT TTCTTGTACA AAGTGGTTGA TATCGGTAAG CCTATCCCTA
3421ACCCTCTCCT CGGTCTCGAT TCTACGTAGT AATGAACTAG TCCGTAACTT GAAAGTATTT
3481CGATTTCTTG GCTTTATATA TCTTGTGGAA AGGACGACCC ACTCTGTAAC CGTTGCGGCC
3541CACGCGTTAA GTCgacaatc aacctctgga ttacaaaatt tgtgaaagat t

Download FASTA (ORF) (Full)