Construct: ORF TRCN0000481299
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF015462.3_s317c1
- Derived from:
- ccsbBroadEn_06727
- DNA Barcode:
- CCCACTCTGTAACCGTTGCGGCCC
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- PIK3CG (5294)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000481299
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 5294 | PIK3CG | phosphatidylinositol-4,5-bi... | NM_001282426.2 | 99.8% | 99.9% | (many diffs) |
| 2 | human | 5294 | PIK3CG | phosphatidylinositol-4,5-bi... | NM_001282427.2 | 99.8% | 99.9% | (many diffs) |
| 3 | human | 5294 | PIK3CG | phosphatidylinositol-4,5-bi... | NM_002649.3 | 99.8% | 99.9% | (many diffs) |
| 4 | human | 5294 | PIK3CG | phosphatidylinositol-4,5-bi... | XM_005250443.3 | 99.8% | 99.9% | (many diffs) |
| 5 | human | 5294 | PIK3CG | phosphatidylinositol-4,5-bi... | XM_017012328.1 | 92.6% | 91.9% | (many diffs) |
| 6 | human | 5294 | PIK3CG | phosphatidylinositol-4,5-bi... | XM_011516316.1 | 91.5% | 91.5% | (many diffs) |
| 7 | human | 5294 | PIK3CG | phosphatidylinositol-4,5-bi... | XM_011516317.1 | 79.8% | 79.4% | (many diffs) |
| 8 | mouse | 30955 | Pik3cg | phosphatidylinositol-4,5-bi... | NM_001146200.1 | 87% | 94% | (many diffs) |
| 9 | mouse | 30955 | Pik3cg | phosphatidylinositol-4,5-bi... | NM_001146201.1 | 87% | 94% | (many diffs) |
| 10 | mouse | 30955 | Pik3cg | phosphatidylinositol-4,5-bi... | NM_020272.2 | 87% | 94% | (many diffs) |
| 11 | mouse | 30955 | Pik3cg | phosphatidylinositol-4,5-bi... | XM_006515112.3 | 87% | 94% | (many diffs) |
| 12 | mouse | 30955 | Pik3cg | phosphatidylinositol-4,5-bi... | XM_006515113.3 | 81.4% | 86.4% | (many diffs) |
| 13 | mouse | 30955 | Pik3cg | phosphatidylinositol-4,5-bi... | XR_872416.1 | 55.4% | (many diffs) | |
| 14 | mouse | 30955 | Pik3cg | phosphatidylinositol-4,5-bi... | XR_001780451.1 | 53.3% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 3372
- ORF length:
- 3306
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatggg gctggagaac tataaacagc ccgtggtgct gagagaggac aactgccgaa 121 ggcgccggag gatgaagccg cgcagtgctg cggccagcct gtcctccatg gagctcatcc 181 ccatcgagtt cgtgctgccc accagccagc gcaaatgcaa gagccccgaa acggcgctgc 241 tgcacgtggc cggccacggc aacgtggagc agatgaaggc ccaggtgtgg ctgcgagcgc 301 tggagaccag cgtggcggcg gacttctacc accggctggg accgcatcac ttcctcctgc 361 tctatcagaa gaaggggcag tggtacgaga tctacgacaa gtaccaggtg gtgcagactc 421 tggactgcct gcgctactgg aaggccacgc accggagccc gggccagatc cacctggtgc 481 agcggcaccc gccctccgag gagtcccaag ccttccagcg gcagctcacg gcgctgattg 541 gctatgacgt cactgacgtc agcaacgtgc acgacgatga gctggagttc acgcgccgtg 601 gcttggtgac cccgcgcatg gcggaggtgg ccagccgcga ccccaagctc tacgccatgc 661 acccgtgggt gacgtccaag cccctcccgg agtacctgtg gaagaagatt gccaacaact 721 gcatcttcat cgtcattcac cgcagcacca ccagccagac cattaaggtc tcacccgacg 781 acacccccgg cgccatcctg cagagcttct tcaccaagat ggccaagaag aaatctctga 841 tggatattcc cgaaagccaa agcgaacagg attttgtgct gcgcgtctgt ggccgggatg 901 agtacctggt gggcgaaacg cccatcaaaa acttccagtg ggtgaggcac tgcctcaaga 961 acggagaaga gattcacgtg gtactggaca cgcctccaga cccggcccta gacgaggtga 1021 ggaaggaaga gtggccgctg gtggacgact gcacgggagt caccggctac catgagcagc 1081 ttaccatcca cggcaaggac cacgagagtg tgttcaccgt gtccctgtgg gactgcgacc 1141 gcaagttcag ggtcaagatc agaggcattg atatccccgt cctgcctcgg aacaccgacc 1201 tcacagtttt tgtagaggca aacatccagc atgggcaaca agtcctttgc caaaggagaa 1261 ccagccccaa acccttcaca gaggaggtgc tgtggaatgt gtggcttgag ttcagtatca 1321 aaatcaaaga cttgcccaaa ggggctctac tgaacctcca gatctactgc ggtaaagctc 1381 cagcactgtc cagcaaggcc tctgcagagt cccccagttc tgagtccaag ggcaaagttc 1441 agcttctcta ttatgtgaac ctgctgctga tagaccaccg tttcctcctg cgccgtggag 1501 aatacgtcct ccacatgtgg cagatatctg ggaagggaga agaccaagga agcttcaatg 1561 ctgacaaact cacgtctgca actaacccag acaaggagaa ctcaatgtcc atctccattc 1621 ttctggacaa ttactgccac ccgatagccc tgcctaagca tcagcccacc cctgacccgg 1681 aaggggaccg ggttcgagca gaaatgccca accagcttcg caagcaattg gaggcgatca 1741 tagccactga tccacttaac cctctcacag cagaggacaa agaattgctc tggcatttta 1801 gatacgaaag ccttaagcac ccaaaagcat atcctaagct atttagttca gtgaaatggg 1861 gacagcaaga aattgtggcc aaaacatacc aattgttggc cagaagggaa gtctgggatc 1921 aaagtgcttt ggatgttggg ttaacaatgc agctcctgga ctgcaacttc tcagatgaaa 1981 atgtaagagc cattgcagtt cagaaactgg agagcttgga ggacgatgat gttctgcatt 2041 accttctaca attggtccag gctgtgaaat ttgaaccata ccatgatagt gcccttgcca 2101 gatttctgct gaagcgtggt ttaagaaaca aaagaattgg tcactttttg ttttggttct 2161 tgagaagtga gatagcccag tccagacact atcagcagag gttcgctgtg attctggaag 2221 cctatctgag gggctgtggc acagccatgc tgcacgactt tacccaacaa gtccaagtaa 2281 tcgagatgtt acaaaaagtc acccttgata ttaaatcgct ctctgctgaa aagtatgacg 2341 tcagttccca agttatttca caacttaaac aaaagcttga aaacctgcag aattctcaac 2401 tccccgaaag ctttagagtt ccatatgatc ctggactgaa agcaggagcg ctggcaattg 2461 aaaaatgtaa agtaatggcc tccaagaaaa aaccactatg gcttgagttt aaatgtgccg 2521 atcctacagc cctatcaaat gaaacaattg gaattatctt taaacatggt gatgatctgc 2581 gccaagacat gcttatttta cagattctac gaatcatgga gtctatttgg gagactgaat 2641 ctttggatct atgcctcctg ccatatggtt gcatttcaac tggtgacaaa ataggaatga 2701 tcgagattgt gaaagacgcc acgacaattg ccaaaattca gcaaagcaca gtgggcaaca 2761 cgggagcatt taaagatgaa gtcctgaatc actggctcaa agaaaaatcc cctactgaag 2821 aaaagtttca ggcagcagtg gagagatttg tttattcctg tgcaggctac tgtgtggcaa 2881 cctttgttct tggaataggc gacagacaca atgacaatat tatgatcacc gagacaggaa 2941 accTATTTCA TATTGACTTC GGGCACATTC TTGGGAATTA CAAAAGTTTC CTGGGCATTA 3001 ATAAAGAGAG AGTGCCATTT GTGCTAACCC CTGACTTCCT CTTTGTGATG GGAACTTCTG 3061 GAAAGAAGAC AAGCCCACAC TTCCAGAAAT TTCAGGACAT CTGTGTTAAG GCTTATCTAG 3121 CCCTTCGTCA TCACACAAAC CTACTGATCA TCCTGTTCTC CATGATGCTG ATGACAGGAA 3181 TGCCCCAGTT AACAAGCAAA GAAGACATTG AATATATCCG GGATGCCCTC ACAGTGGGGA 3241 AAAATGAGGA GGATGCTAAA AAGTATTTTC TTGATCAGAT CGAAGTTTGC AGAGACAAAG 3301 GATGGACTGT GCAGTTTAAT TGGTTTCTAC ATCTTGTTCT TGGCATCAAA CAAGGAGAGA 3361 AACATTCAGC CTACCCAACT TTCTTGTACA AAGTGGTTGA TATCGGTAAG CCTATCCCTA 3421 ACCCTCTCCT CGGTCTCGAT TCTACGTAGT AATGAACTAG TCCGTAACTT GAAAGTATTT 3481 CGATTTCTTG GCTTTATATA TCTTGTGGAA AGGACGACCC ACTCTGTAAC CGTTGCGGCC 3541 CACGCGTTAA GTCgacaatc aacctctgga ttacaaaatt tgtgaaagat t