Construct: ORF TRCN0000481530
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF017973.1_s317c1
- Derived from:
- ccsbBroadEn_04917
- DNA Barcode:
- CGTATCAGCACGACACGCGAAGAC
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- C9orf85 (138241)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000481530
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 138241 | C9orf85 | chromosome 9 open reading f... | NM_182505.5 | 100% | 100% | |
| 2 | human | 138241 | C9orf85 | chromosome 9 open reading f... | NM_001365055.2 | 84.9% | 81% | (many diffs) |
| 3 | human | 138241 | C9orf85 | chromosome 9 open reading f... | NM_001365053.2 | 71.4% | 63.3% | (many diffs) |
| 4 | human | 138241 | C9orf85 | chromosome 9 open reading f... | NM_001365057.2 | 43.9% | 43.9% | 207_208ins264 |
| 5 | human | 138241 | C9orf85 | chromosome 9 open reading f... | NR_157410.2 | 35.8% | 1_96del;196_325del;698_1315del | |
| 6 | human | 138241 | C9orf85 | chromosome 9 open reading f... | NR_157409.2 | 10.6% | (many diffs) | |
| 7 | mouse | 66206 | 1110059E24Rik | RIKEN cDNA 1110059E24 gene | NM_025423.2 | 83.4% | 81.5% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 537
- ORF length:
- 471
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgag ctcccagaaa ggcaacgtgg ctcgttccag accTCAGAAG CACCAGAATA 121 CGTTTAGCTT CAAAAATGAC AAGTTCGATA AAAGTGTGCA GACCAAGAAA ATTAATGCAA 181 AACTTCATGA TGGAGTATGT CAGCGCTGTA AAGAAGTTCT TGAGTGGCGT GTAAAATACA 241 GCAAATACAA ACCATTATCA AAACCTAAAA AGTGTGTTAA ATGTTTACAA AAGACAGTGA 301 AGGATTCTTA TCACATAATG TGCAGGCCAT GTGCCTGTGA ACTTGAAGTT TGCGCAAAAT 361 GTGGAAAGAA AGAAGACATT GTTATTCCGT TGAATAAAGA AACAGAAAAA ATAGAACATA 421 CTGAAAATAA TCTAAGTTCC AACCATAGAA GAAGCTGCAG AAGAAATGAA GAAAGTGATG 481 ATGATTTAGA TTTTGATATT GATTTAGAAG ACACAGGAGG AGACCATCAA ATGAATTACC 541 CAACTTTCTT GTACAAAGTG GTTGATATCG GTAAGCCTAT CCCTAACCCT CTCCTCGGTC 601 TCGATTCTAC GTAGTAATGA ACTAGTCCGT AACTTGAAAG TATTTCGATT TCTTGGCTTT 661 ATATATCTTG TGGAAAGGAC GACGTATCAG CACGACACGC GAAGACACGC GTTAAGTCga 721 caatcaacct ctggattaca aaatttgtga aagatt