Construct: ORF TRCN0000481572
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF011315.2_s317c1
- Derived from:
- ccsbBroadEn_02305
- DNA Barcode:
- GTGAACGCAATAACATTAGTACCT
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- GPC6 (10082)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000481572
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 10082 | GPC6 | glypican 6 | NM_005708.5 | 100% | 100% | |
| 2 | human | 10082 | GPC6 | glypican 6 | XM_011521044.2 | 87.3% | 87.3% | 0_1ins210 |
| 3 | human | 10082 | GPC6 | glypican 6 | XM_017020298.1 | 87.3% | 87.3% | 0_1ins210 |
| 4 | human | 10082 | GPC6 | glypican 6 | XM_017020299.2 | 87.3% | 87.3% | 0_1ins210 |
| 5 | human | 10082 | GPC6 | glypican 6 | XM_017020300.1 | 87.3% | 87.3% | 0_1ins210 |
| 6 | human | 10082 | GPC6 | glypican 6 | XM_017020301.1 | 78% | 78% | 0_1ins366 |
| 7 | human | 10082 | GPC6 | glypican 6 | XM_017020302.1 | 54.1% | 49.8% | (many diffs) |
| 8 | mouse | 23888 | Gpc6 | glypican 6 | NM_011821.3 | 88.5% | 96.7% | (many diffs) |
| 9 | mouse | 23888 | Gpc6 | glypican 6 | NM_001079844.2 | 86.9% | 95% | (many diffs) |
| 10 | mouse | 23888 | Gpc6 | glypican 6 | XM_006518929.3 | 48.9% | 52.2% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 1734
- ORF length:
- 1665
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcaccat gccttcttgg atcggggctg tgattcttcc cctcttgggg ctgctgctct 121 ccctccccgc cggggcggat gtgaaggctc ggagctgcgg agaggtccgc caggcgtacg 181 gtgccaaggg attcagcctg gcggacatcc cctaccagga gatcgcaggg gaacacttaa 241 gaatctgtcc tcaggaatat acatgctgca ccacagaaat ggaagacaag ttaagccaac 301 aaagcaaact cgaatttgaa aaccttgtgg aagagacaag ccattttgtg cgcaccactt 361 ttgtgtccag gcataagaaa tttgacgaat ttttccgaga gctcctggag aatgcagaaa 421 agtcactaaa tgatatgttt gtacggacct atggcatgct gtacatgcag aattcagaag 481 tcttccagga cctcttcaca gagctgaaaa ggtactacac tgggggtaat gtgaatctgg 541 aggaaatgct caatgacttt tgggctcggc tcctggaacg gatgtttcag ctgataaacc 601 ctcagtatca cttcagtgaa gactacctgg aatgtgtgag caaatacact gaccagctca 661 agccatttgg agacgtgccc cggaaactga agattcaggt tacccgcgcc ttcattgctg 721 ccaggacctt tgtccagggg ctgactgtgg gcagagaagt tgcaaaccga gtttccaagg 781 tcagcccaac cccagggtgt atccgtgccc tcatgaagat gctgtactgc ccatactgtc 841 gggggcttcc cactgtgagg ccctgcaaca actactgtct caacgtcatg aagggctgct 901 tggcaaatca ggctgacctc gacacagagt ggaatctgtt tatagatgca atgctcttgg 961 tggcagagcg actggagggg ccattcaaca ttgagtcggt catggacccg atagatgtca 1021 agatttctga agccattatg aacatgcaag aaaacagcat gcaggtgtct gcaaaggtct 1081 ttcagggatg tggtcagccc aaacctgctc cagccctcag atctgcccgc tcagctcctg 1141 aaaattttaa tacacgtttc aggccctaca atcctgagga aagaccaaca actgctgcag 1201 gcacaagctt ggaccggctg gtcacagaca taaaagagaa attgaagctc tctaaaaagg 1261 tctggtcagc attaCCCTAC ACTATCTGCA AGGACGAGAG CGTGACAGCG GGCACGTCCA 1321 ACGAGGAGGA ATGCTGGAAC GGGCACAGCA AAGCCAGATA CTTGCCTGAG ATCATGAATG 1381 ATGGGCTCAC CAACCAGATC AACAATCCCG AGGTGGATGT GGACATCACT CGGCCTGACA 1441 CTTTCATCAG ACAGCAGATT ATGGCTCTCC GTGTGATGAC CAACAAACTA AAAAACGCCT 1501 ACAATGGCAA TGATGTCAAT TTCCAGGACA CAAGTGATGA ATCCAGTGGC TCAGGGAGTG 1561 GCAGTGGGTG CATGGATGAC GTGTGTCCCA CGGAGTTTGA GTTTGTCACC ACAGAGGCCC 1621 CCGCAGTGGA TCCCGACCGG AGAGAGGTGG ACTCTTCTGC AGCCCAGCGT GGCCACTCCC 1681 TGCTCTCCTG GTCTCTCACC TGCATTGTCC TGGCACTGCA GAGACTGTGC AGATTGCCAA 1741 CTTTCTTGTA CAAAGTGGTT GATATCGGTA AGCCTATCCC TAACCCTCTC CTCGGTCTCG 1801 ATTCTACGTA GTAATGAACT AGTCCGTAAC TTGAAAGTAT TTCGATTTCT TGGCTTTATA 1861 TATCTTGTGG AAAGGACGAG TGAACGCAAT AACATTAGTA CCTACGCGTT AAGTCgacaa 1921 tcaacctctg gattacaaaa tttgtgaaag att