Construct: ORF TRCN0000487708

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF021735.1_s317c1
DNA Barcode:
AAGTTCAAGTCTTTTTGTAGGCCG
Epitope Tag:
V5 (not translated due to prior stop codon)
Notes:
Has stop codon in insert

Originally Annotated References:

Gene:
FGFR2 (2263)

Vector Information:

Vector Backbone:
pLX_317
Pol II Cassette 1:
SV40-PuroR
Pol II Cassette 2:
EF1a-TRCN0000487708
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
V5

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 2263 FGFR2 fibroblast growth factor re... XM_024447892.1 89% 89% 1_141del
2 human 2263 FGFR2 fibroblast growth factor re... NM_001320654.1 64.7% 64.7% 1_627del
3 human 2263 FGFR2 fibroblast growth factor re... NM_001144918.2 54.5% 54.5% 1_960del
4 human 2263 FGFR2 fibroblast growth factor re... NM_001144917.2 54.4% 54.4% 1_963del
5 human 2263 FGFR2 fibroblast growth factor re... NM_001144916.1 54.3% 54.3% 1_966del
6 human 2263 FGFR2 fibroblast growth factor re... NM_001144914.1 54.1% 54.1% 1_975del
7 human 2263 FGFR2 fibroblast growth factor re... XM_017015924.2 52.9% 52.9% 1_1023del
8 human 2263 FGFR2 fibroblast growth factor re... XM_024447891.1 52.8% 52.8% 1_1026del
9 human 2263 FGFR2 fibroblast growth factor re... NM_023029.2 52.4% 52.4% 1_1044del
10 human 2263 FGFR2 fibroblast growth factor re... XM_024447889.1 51.2% 51.2% 1_1095del
11 human 2263 FGFR2 fibroblast growth factor re... XM_024447888.1 51.2% 51.2% 1_1098del
12 human 2263 FGFR2 fibroblast growth factor re... XM_024447887.1 51.1% 51.1% 1_1101del
13 human 2263 FGFR2 fibroblast growth factor re... XM_024447890.1 51% 51% 1_1104del
14 human 2263 FGFR2 fibroblast growth factor re... XM_017015925.2 48.1% 47.9% (many diffs)
15 human 2263 FGFR2 fibroblast growth factor re... NM_001144915.1 47.3% 45.7% (many diffs)
16 human 2263 FGFR2 fibroblast growth factor re... NM_001320658.2 46.8% 46.8% 1_1305del
17 human 2263 FGFR2 fibroblast growth factor re... NM_000141.4 46.7% 46.7% 1_1311del
18 human 2263 FGFR2 fibroblast growth factor re... NM_022970.3 46.7% 46.7% 1_1314del
19 human 2263 FGFR2 fibroblast growth factor re... XM_006717708.3 45.7% 45.7% 1_1365del
20 human 2263 FGFR2 fibroblast growth factor re... NM_001144919.2 45.1% 45% 1_1047del;2037_2038insGA;2040_2040delCins158
21 human 2263 FGFR2 fibroblast growth factor re... XM_017015921.2 41.5% 41.4% (many diffs)
22 human 2263 FGFR2 fibroblast growth factor re... XM_017015920.2 41.4% 41.3% (many diffs)
23 human 2263 FGFR2 fibroblast growth factor re... XM_006717710.4 41.3% 41.2% (many diffs)
24 human 2263 FGFR2 fibroblast growth factor re... NM_001144913.1 40.2% 40.1% 1_1314del;2304_2305insGA;2307_2307delCins158
25 human 2263 FGFR2 fibroblast growth factor re... NR_073009.2 26% 1_1747del;2900_4427del
26 mouse 14183 Fgfr2 fibroblast growth factor re... NM_001347638.1 48.4% 52.8% (many diffs)
27 mouse 14183 Fgfr2 fibroblast growth factor re... XM_011241677.2 48.3% 52.7% (many diffs)
28 mouse 14183 Fgfr2 fibroblast growth factor re... XM_006507357.3 48.3% 52.6% (many diffs)
29 mouse 14183 Fgfr2 fibroblast growth factor re... NM_201601.2 48.2% 52.6% (many diffs)
30 mouse 14183 Fgfr2 fibroblast growth factor re... XM_011241676.2 46.7% 50.9% (many diffs)
31 mouse 14183 Fgfr2 fibroblast growth factor re... XM_011241675.2 46.6% 50.8% (many diffs)
32 mouse 14183 Fgfr2 fibroblast growth factor re... XM_006507354.3 46.5% 50.7% (many diffs)
33 mouse 14183 Fgfr2 fibroblast growth factor re... XM_011241674.2 41.8% 45.5% (many diffs)
34 mouse 14183 Fgfr2 fibroblast growth factor re... XM_006507350.3 41.7% 45.5% (many diffs)
35 mouse 14183 Fgfr2 fibroblast growth factor re... NM_010207.2 41.7% 45.4% (many diffs)
36 mouse 14183 Fgfr2 fibroblast growth factor re... XM_006507349.2 41.6% 45.4% (many diffs)
37 mouse 14183 Fgfr2 fibroblast growth factor re... XM_011241673.2 41.6% 45.4% (many diffs)
38 mouse 14183 Fgfr2 fibroblast growth factor re... XM_017321975.1 41.6% 45.4% (many diffs)
39 mouse 14183 Fgfr2 fibroblast growth factor re... XM_017321977.1 38% 41.2% (many diffs)
40 mouse 14183 Fgfr2 fibroblast growth factor re... XM_017321976.1 37.9% 41.2% (many diffs)
41 mouse 14183 Fgfr2 fibroblast growth factor re... XM_006507352.2 37.8% 41.1% (many diffs)
42 mouse 14183 Fgfr2 fibroblast growth factor re... XR_001785477.1 15.7% (many diffs)
43 mouse 14183 Fgfr2 fibroblast growth factor re... XR_001785478.1 15.5% (many diffs)
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Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
153
ORF end:
1305
ORF length:
1152
Sequence:
1tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag
61caggcttcag cagccagccg gctgtgcaca agctgaccaa acgtatcccc ctgcggagac
121aggtaacagt ttcggctgag tccagctcct ccatgaactc caacaccccg ctggtgagga
181taacaacacg cctctcttca acggcagaca cccccatgct ggcaggggtc tccgagtatg
241aacttccaga ggacccaaaa tgggagtttc caagagataa gctgacactg ggcaagcccc
301tgggagaagg ttgctttggg caagtggtca tggcggaagc agtgggaatt gacaaagaca
361agcccaagga ggcggtcacc gtggccgtga agatgttgaa agatgatgcc acagagaaag
421acctttctga tctggtgtca gagatggaga tgatgaagat gattgggaaa cacaagaata
481tcataaatct tcttggagcc tgcacacagg atgggcctct ctatgtcata gttgagtatg
541cctctaaagg caacctccga gaatacctcc gagcccggag gccacccggg atggagtact
601cctatgacat taaccgtgtt cctgaggagc agatgacctt caaggacttg gtgtcatgca
661cctaccagct ggccagaggc atggagtact tggcttccca aaaatgtatt catcgagatt
721tagcagccag aaatgttttg gtaacagaaa acaatgtgat gaaaatagca gactttggac
781tcgccagaga tatcaacaat atagactatt acaaaaagac caccaatggg cggcttccag
841tcaagtggat ggctccagaa gccctgtttg atagagtata cactcatcag agtgatgtct
901ggtccttcgg ggtgttaatg tgggagatct tcactttagg gggctcgccc tacccaggga
961ttcccgtgga ggaacttttt aagctgctga aggaaggaca cagaatggat aagccagcca
1021actgcaccaa cgaactgtac atgatgatga gggactgttg gcatgcagtg ccctcccaga
1081gaccaacgtt caagcagttg gtagaagact tggatcgaaT TCTCACTCTC ACAACCAATG
1141AGGAATACTT GGACCTCAGC CAACCTCTCG AACAGTATTC ACCTAGTTAC CCTGACACAA
1201GAAGTTCTTG TTCTTCAGGA GATGATTCTG TTTTTTCTCC AGACCCCATG CCTTACGAAC
1261CATGCCTTCC TCAGTATCCA CACATAAACG GCAGTGTTAA AACATGAGAC CCAGCTTTCT
1321TGTACAAAGT GGTTGATATC GGTAAGCCTA TCCCTAACCC TCTCCTCGGT CTCGATTCTA
1381CGTAGTAATG AACTAGTCCG TAACTTGAAA GTATTTCGAT TTCTTGGCTT TATATATCTT
1441GTGGAAAGGA CGAAAGTTCA AGTCTTTTTG TAGGCCGACG CGTTAAGTCg acaatcaacc
1501tctggattac aaaatttgtg aaagatt

Download FASTA (ORF) (Full)