Construct: ORF TRCN0000487793
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF020560.1_s317c1
- DNA Barcode:
- TGGAGTGGCTTACGTAAATCTGCC
- Epitope Tag:
- V5 (not translated due to prior stop codon)
- Notes:
- Has stop codon in insert
Originally Annotated References:
- Gene:
- MAP4K4 (9448)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000487793
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 9448 | MAP4K4 | mitogen-activated protein k... | XM_017005364.1 | 99.9% | 99.9% | 1808_1809insCAG |
| 2 | human | 9448 | MAP4K4 | mitogen-activated protein k... | XM_017005363.1 | 99.7% | 99.7% | 2062_2070delGGCGAAGTG |
| 3 | human | 9448 | MAP4K4 | mitogen-activated protein k... | XM_005264064.3 | 99.3% | 99.3% | 3370_3393del |
| 4 | human | 9448 | MAP4K4 | mitogen-activated protein k... | XM_005264062.3 | 99.1% | 99.1% | 2062_2070delGGCGAAGTG;3379_3402del |
| 5 | human | 9448 | MAP4K4 | mitogen-activated protein k... | XM_005264063.3 | 99% | 99% | 1808_1809insCAG;2059_2067delGGCGAAGTG;3376_3399del |
| 6 | human | 9448 | MAP4K4 | mitogen-activated protein k... | XM_006712868.2 | 97.2% | 97.2% | 1481_1482ins93;1969_1977delGGCGAAGTG |
| 7 | human | 9448 | MAP4K4 | mitogen-activated protein k... | XM_005264068.3 | 96.8% | 96.8% | 1481_1482ins93;3277_3300del |
| 8 | human | 9448 | MAP4K4 | mitogen-activated protein k... | XM_005264065.3 | 96.6% | 96.6% | 1481_1482ins93;1969_1977delGGCGAAGTG;3286_3309del |
| 9 | human | 9448 | MAP4K4 | mitogen-activated protein k... | XM_005264066.3 | 96.5% | 96.5% | (many diffs) |
| 10 | human | 9448 | MAP4K4 | mitogen-activated protein k... | XM_017005352.1 | 95.6% | 95.6% | 1703_1864del;2224_2232delGGCGAAGTG |
| 11 | human | 9448 | MAP4K4 | mitogen-activated protein k... | XM_017005353.1 | 95.5% | 95.5% | 1703_1864del;1970_1971insCAG;2221_2229delGGCGAAGTG |
| 12 | human | 9448 | MAP4K4 | mitogen-activated protein k... | XM_005264058.3 | 95.2% | 95.2% | 1703_1864del;3532_3555del |
| 13 | human | 9448 | MAP4K4 | mitogen-activated protein k... | XM_017005351.1 | 95.1% | 95.1% | 1703_1864del;1970_1971insCAG;3529_3552del |
| 14 | human | 9448 | MAP4K4 | mitogen-activated protein k... | XM_005264057.3 | 95% | 95% | 1703_1864del;2224_2232delGGCGAAGTG;3541_3564del |
| 15 | human | 9448 | MAP4K4 | mitogen-activated protein k... | XM_017005371.1 | 94.8% | 94.8% | 2356_2357ins192 |
| 16 | human | 9448 | MAP4K4 | mitogen-activated protein k... | XM_005264071.2 | 94.8% | 94.8% | 1808_1809insCAG;2353_2354ins192 |
| 17 | human | 9448 | MAP4K4 | mitogen-activated protein k... | XM_006712869.2 | 94.6% | 94.6% | 2062_2070delGGCGAAGTG;2365_2366ins192 |
| 18 | human | 9448 | MAP4K4 | mitogen-activated protein k... | XM_017005370.1 | 94.5% | 94.5% | 1808_1809insCAG;2059_2067delGGCGAAGTG;2362_2363ins192 |
| 19 | human | 9448 | MAP4K4 | mitogen-activated protein k... | XM_017005350.1 | 93.9% | 93.9% | 1704_1934del;2293_2301delGGCGAAGTG |
| 20 | human | 9448 | MAP4K4 | mitogen-activated protein k... | XM_017005369.1 | 93.9% | 93.9% | (many diffs) |
| 21 | human | 9448 | MAP4K4 | mitogen-activated protein k... | NM_145686.3 | 93.4% | 93.4% | 1481_1482ins93;1610_1771del |
| 22 | human | 9448 | MAP4K4 | mitogen-activated protein k... | XM_005264050.3 | 93.4% | 93.4% | 1704_1934del;2293_2301delGGCGAAGTG;3610_3633del |
| 23 | human | 9448 | MAP4K4 | mitogen-activated protein k... | XM_005264052.3 | 93.3% | 93.3% | (many diffs) |
| 24 | human | 9448 | MAP4K4 | mitogen-activated protein k... | XM_017005357.1 | 93.2% | 93.2% | 1481_1482ins93;1610_1771del;2131_2139delGGCGAAGTG |
| 25 | human | 9448 | MAP4K4 | mitogen-activated protein k... | XM_017005358.1 | 93.1% | 93.1% | (many diffs) |
| 26 | human | 9448 | MAP4K4 | mitogen-activated protein k... | XM_017005356.1 | 92.9% | 92.9% | 1481_1482ins93;1610_1771del;3439_3462del |
| 27 | human | 9448 | MAP4K4 | mitogen-activated protein k... | XM_005264060.3 | 92.8% | 92.8% | (many diffs) |
| 28 | human | 9448 | MAP4K4 | mitogen-activated protein k... | XM_005264059.3 | 92.6% | 92.6% | (many diffs) |
| 29 | human | 9448 | MAP4K4 | mitogen-activated protein k... | XM_017005375.1 | 92.4% | 92.4% | 1481_1482ins93;2263_2264ins192 |
| 30 | human | 9448 | MAP4K4 | mitogen-activated protein k... | XM_017005374.1 | 92.1% | 92.1% | 1481_1482ins93;1969_1977delGGCGAAGTG;2272_2273ins192 |
| 31 | human | 9448 | MAP4K4 | mitogen-activated protein k... | XM_017005355.1 | 91.8% | 91.8% | 1481_1482ins93;1611_1841del |
| 32 | human | 9448 | MAP4K4 | mitogen-activated protein k... | XM_017005373.1 | 91.8% | 91.8% | 1481_1482ins93;2263_2264ins192;3085_3108del |
| 33 | human | 9448 | MAP4K4 | mitogen-activated protein k... | XM_017005354.1 | 91.6% | 91.6% | 1481_1482ins93;1611_1841del;2200_2208delGGCGAAGTG |
| 34 | human | 9448 | MAP4K4 | mitogen-activated protein k... | XM_017005372.1 | 91.5% | 91.5% | (many diffs) |
| 35 | human | 9448 | MAP4K4 | mitogen-activated protein k... | NM_004834.5 | 91.5% | 91.5% | (many diffs) |
| 36 | human | 9448 | MAP4K4 | mitogen-activated protein k... | XM_006712865.2 | 91.1% | 91.1% | (many diffs) |
| 37 | human | 9448 | MAP4K4 | mitogen-activated protein k... | NM_001242559.2 | 90.8% | 90.8% | 1703_1864del;1970_1971insCAG;2515_2516ins192 |
| 38 | human | 9448 | MAP4K4 | mitogen-activated protein k... | XM_017005365.1 | 90.7% | 90.7% | 1703_1864del;2224_2232delGGCGAAGTG;2527_2528ins192 |
| 39 | human | 9448 | MAP4K4 | mitogen-activated protein k... | XM_005264048.2 | 90.3% | 90.3% | 1704_2096del;2455_2463delGGCGAAGTG |
| 40 | human | 9448 | MAP4K4 | mitogen-activated protein k... | XM_017005361.1 | 89.1% | 89.1% | 1704_1934del;2293_2301delGGCGAAGTG;2596_2597ins192 |
| 41 | human | 9448 | MAP4K4 | mitogen-activated protein k... | XM_017005362.1 | 89% | 89% | (many diffs) |
| 42 | human | 9448 | MAP4K4 | mitogen-activated protein k... | XM_017005359.1 | 88.6% | 88.6% | (many diffs) |
| 43 | human | 9448 | MAP4K4 | mitogen-activated protein k... | XM_005264069.2 | 88.5% | 88.5% | 1481_1482ins93;1610_1771del;2425_2426ins192 |
| 44 | human | 9448 | MAP4K4 | mitogen-activated protein k... | XM_017005360.1 | 88.5% | 88.5% | (many diffs) |
| 45 | human | 9448 | MAP4K4 | mitogen-activated protein k... | NM_145687.4 | 88.3% | 88.3% | (many diffs) |
| 46 | human | 9448 | MAP4K4 | mitogen-activated protein k... | XM_017005368.1 | 88.2% | 88.2% | (many diffs) |
| 47 | human | 9448 | MAP4K4 | mitogen-activated protein k... | XM_017005349.1 | 88% | 88% | (many diffs) |
| 48 | human | 9448 | MAP4K4 | mitogen-activated protein k... | NM_001242560.2 | 86.8% | 86.8% | (many diffs) |
| 49 | human | 9448 | MAP4K4 | mitogen-activated protein k... | XM_017005367.1 | 86.7% | 86.7% | (many diffs) |
| 50 | human | 9448 | MAP4K4 | mitogen-activated protein k... | XM_017005366.1 | 86.3% | 86.3% | (many diffs) |
| 51 | mouse | 26921 | Map4k4 | mitogen-activated protein k... | XM_017321021.1 | 90.7% | 98.3% | (many diffs) |
| 52 | mouse | 26921 | Map4k4 | mitogen-activated protein k... | XM_017321018.1 | 90.5% | 98% | (many diffs) |
| 53 | mouse | 26921 | Map4k4 | mitogen-activated protein k... | NM_001252201.1 | 88% | 95.6% | (many diffs) |
| 54 | mouse | 26921 | Map4k4 | mitogen-activated protein k... | XM_017321011.1 | 86.7% | 94% | (many diffs) |
| 55 | mouse | 26921 | Map4k4 | mitogen-activated protein k... | XM_017321013.1 | 85.4% | 90% | (many diffs) |
| 56 | mouse | 26921 | Map4k4 | mitogen-activated protein k... | XM_017321004.1 | 85.3% | 92.5% | (many diffs) |
| 57 | mouse | 26921 | Map4k4 | mitogen-activated protein k... | XM_006496042.1 | 85.2% | 92.4% | (many diffs) |
| 58 | mouse | 26921 | Map4k4 | mitogen-activated protein k... | XM_006496043.1 | 84.5% | 91.8% | (many diffs) |
| 59 | mouse | 26921 | Map4k4 | mitogen-activated protein k... | XM_017321016.1 | 84.3% | 91.6% | (many diffs) |
| 60 | mouse | 26921 | Map4k4 | mitogen-activated protein k... | XM_006496046.1 | 82.8% | 90.4% | (many diffs) |
| 61 | mouse | 26921 | Map4k4 | mitogen-activated protein k... | XM_017321002.1 | 82.2% | 86.5% | (many diffs) |
| 62 | mouse | 26921 | Map4k4 | mitogen-activated protein k... | XM_017321001.1 | 82.1% | 89% | (many diffs) |
| 63 | mouse | 26921 | Map4k4 | mitogen-activated protein k... | XM_017320998.1 | 82% | 88.9% | (many diffs) |
| 64 | mouse | 26921 | Map4k4 | mitogen-activated protein k... | XM_006496044.1 | 82% | 89.2% | (many diffs) |
| 65 | mouse | 26921 | Map4k4 | mitogen-activated protein k... | XM_006496041.1 | 81.9% | 88.8% | (many diffs) |
| 66 | mouse | 26921 | Map4k4 | mitogen-activated protein k... | XM_017320994.1 | 81.8% | 88.7% | (many diffs) |
| 67 | mouse | 26921 | Map4k4 | mitogen-activated protein k... | NM_008696.2 | 80.6% | 85.2% | (many diffs) |
| 68 | mouse | 26921 | Map4k4 | mitogen-activated protein k... | NM_001252202.1 | 79.7% | 86.9% | (many diffs) |
| 69 | mouse | 26921 | Map4k4 | mitogen-activated protein k... | XM_006496045.1 | 79.5% | 86.7% | (many diffs) |
| 70 | mouse | 26921 | Map4k4 | mitogen-activated protein k... | NM_001252200.1 | 77.6% | 81.9% | (many diffs) |
| 71 | mouse | 26921 | Map4k4 | mitogen-activated protein k... | XM_017321010.1 | 77.5% | 84.3% | (many diffs) |
| 72 | mouse | 26921 | Map4k4 | mitogen-activated protein k... | XM_011238530.1 | 77.3% | 84.1% | (many diffs) |
| 73 | mouse | 26921 | Map4k4 | mitogen-activated protein k... | XR_001784730.1 | 57.2% | (many diffs) | |
| 74 | mouse | 26921 | Map4k4 | mitogen-activated protein k... | XR_001784729.1 | 55% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 84
- ORF end:
- 3834
- ORF length:
- 3750
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 caggctccgc ggcccccttc accatggcga acgactcccc tgcaaaaagt ctggtggaca 121 tcgacctctc ctccctgcgg gatcctgctg ggatttttga gctggtggaa gtggttggaa 181 atggcaccta tggacaagtc tataagggtc gacatgttaa aacgggtcag ttggcagcca 241 tcaaagttat ggatgtcact gaggatgaag aggaagaaat caaactggag ataaatatgc 301 taaagaaata ctctcatcac agaaacattg caacatatta tggtgctttc atcaaaaaga 361 gccctccagg acatgatgac caactctggc ttgttatgga gttctgtggg gctgggtcca 421 ttacagacct tgtgaagaac accaaaggga acacactcaa agaagactgg atcgcttaca 481 tctccagaga aatcctgagg ggactggcac atcttcacat tcatcatgtg attcaccggg 541 atatcaaggg ccagaatgtg ttgctgactg agaatgcaga ggtgaaactt gttgactttg 601 gtgtgagtgc tcagctggac aggactgtgg ggcggagaaa tacgttcata ggcactccct 661 actggatggc tcctgaggtc atcgcctgtg atgagaaccc agatgccacc tatgattaca 721 gaagtgatct ttggtcttgt ggcattacag ccattgagat ggcagaaggt gctccccctc 781 tctgtgacat gcatccaatg agagcactgt ttctcattcc cagaaaccct cctccccggc 841 tgaagtcaaa aaaatggtcg aagaagtttt ttagttttat agaagggtgc ctggtgaaga 901 attacatgca gcggccctct acagagcagc ttttgaaaca tccttttata agggatcagc 961 caaatgaaag gcaagttaga atccagctta aggatcatat agatcgtacc aggaagaaga 1021 gaggcgagaa agatgaaact gagtatgagt acagtgggag tgaggaagaa gaggaggaag 1081 tgcctgaaca ggaaggagag ccaagttcca ttgtgaacgt gcctggtgag tctactcttc 1141 gccgagattt cctgagactg cagcaggaga acaaggaacg ttccgaggct cttcggagac 1201 aacagttact acaggagcaa cagctccggg agcaggaaga atataaaagg caactgctgg 1261 cagagagaca gaagcggatt gagcagcaga aagaacagag gcgacggcta gaagagcaac 1321 aaaggagaga gcgggaagct agaaggcagc aggaacgtga acagcgaagg agagaacaag 1381 aagaaaagag gcgtctagag gagttggaga gaaggcgcaa agaagaagag gagaggagac 1441 gggcagaaga agaaaagagg agagttgaaa gagaacagga gtatatcagg cgacagctag 1501 aagaggagca gcggcacttg gaagtccttc agcagcagct gctccaggag caggccatgt 1561 tactggagtg ccgatggcgg gagatggagg agcaccggca ggcagagagg ctccagaggc 1621 agttgcaaca agaacaagca tatctcctgt ctctacagca tgaccatagg aggccgcacc 1681 cgcagcactc gcagcagccg ccaccaccgc agcaggaaag gagcaagcca agcttccatg 1741 ctcccgagcc caaagcccac tacgagcctg ctgaccgagc gcgagaggtt cctgtgagaa 1801 caacatctcg ctcccctgtt ctgtcccgtc gagattcccc actgcagggc agtgggcagc 1861 agaatagcca ggcaggacag agaaactcca ccagcagtat tgagcccagg cttctgtggg 1921 agagagtgga gaagctggtg cccagacctg gcagtggcag ctcctcaggg tccagcaact 1981 caggatccca gcccgggtct caccctgggt ctcagagtgg ctccggggaa cgcttcagag 2041 tgagatcatc atccaagtct gaaggctctc catctcagcg cctggaaaat gcagtgaaaa 2101 aacctgaaga taaaaaggaa gttttcagac ccctcaagcc tgctgatctg accgcactgg 2161 ccaaagagct tcgagcagtg gaagatgtac ggccacctca caaagtaacg gactactcct 2221 catccagtga ggagtcgggg acgacggatg aggaggacga cgatgtggag caggaagggg 2281 ctgacgagtc cacctcagga ccagaggaca ccagagcagc gtcatctctg aatttgagca 2341 atggtgaaac ggaatctgtg aaaaccatga ttgtccatga tgatgtagaa agtgagccgg 2401 ccatgacccc atccaaggag ggcactctaa tcgtccgcca gagtacagtt gaccaaaagc 2461 gtgccagcca tcatgagagc aatggctttg ccggtcgcat tcacctcttg ccagatctct 2521 tacagcaaag ccattcctcc tccacttcct ccacctcctc ctccccatcc tccagccagc 2581 cgacacccac catgtcccca cagacacccc aggacaagct cactgctaat gagactcagt 2641 ccgctagtag cacactccag aaacacaaat cttcctcctc ctttacacct tttatagacc 2701 ccagattact acagatttct ccatctagcg gaacaacagt gacatctgtg gtgggatttt 2761 cctgtgatgg gatgagacca gaagccataa ggcaagatcc tacccggaaa ggctcagtgg 2821 tcaatgtgaa tcctaccaac actaggccac agagtgacac cccggagatt cgtaaataca 2881 agaagaggtt taactctgag attctgtgtg ctgccttatg gggagtgaat ttgctagtgg 2941 gtacagagag tggcctgatg ctgctggaca gaagtggcca agggaaggtc tatcctctta 3001 tcaaccgaag acgatttcaa caaatggacg tacttgaggg cttgaatgtc ttggtgacaa 3061 tatctggcaa aaaggataag ttacgtgtct actatttgtc ctggttaaga aataaaatac 3121 ttcacaatga tccagaagtt gagaagaagc agggatggac aaccgtaggg gatttggaag 3181 gatgtgtaca ttataaagtt gtaaaatatg aaagaatcaa atttctggtg attgctttga 3241 agagttctgt ggaagtctat gcgtgggcac caaagccata tcacaaattt atggccttta 3301 agtcatttgg agaattggta cataagccat tactggtgga tctcactgtt gaggaaggcc 3361 agaggttgaa agtgatctat ggatcctgtg ctggattcca tgctgttgat gtggattcag 3421 gatcagtcta tgacatttat ctaccaacac atatccagtg tagcatcaaa ccccatgcaa 3481 tcatcatcct ccccaataca gatggaatgg agcttctggt gtgctatgaa gatgaggggg 3541 tttatgtaaa cacatatgga aggatcacca aggatgtagt tctacagtgG GGAGAGATGC 3601 CTACATCAGT AGCATATATT CGATCCAATC AGACAATGGG CTGGGGAGAG AAGGCCATAG 3661 AGATCCGATC TGTGGAAACT GGTCACTTGG ATGGTGTGTT CATGCACAAA AGGGCTCAAA 3721 GACTAAAATT CTTGTGTGAA CGCAATGACA AGGTGTTCTT TGCCTCTGTT CGGTCTGGTG 3781 GCAGCAGTCA GGTTTATTTC ATGACCTTAG GCAGGACTTC TCTTCTGAGC TGGTAGACTA 3841 GTAAGGGTGG GCGCGCCGAC CCAGCTTTCT TGTACAAAGT GGTTGATATC GGTAAGCCTA 3901 TCCCTAACCC TCTCCTCGGT CTCGATTCTA CGTAGTAATG AACTAGTCCG TAACTTGAAA 3961 GTATTTCGAT TTCTTGGCTT TATATATCTT GTGGAAAGGA CGATGGAGTG GCTTACGTAA 4021 ATCTGCCACG CGTTAAGTCg acaatcaacc tctggattac aaaatttgtg aaagatt