Transcript: Human NM_001242560.2

Homo sapiens mitogen-activated protein kinase kinase kinase kinase 4 (MAP4K4), transcript variant 5, mRNA.

Source:
NCBI, updated 2019-07-31
Taxon:
Homo sapiens (human)
Gene:
MAP4K4 (9448)
Length:
7523
CDS:
375..4082

Additional Resources:

NCBI RefSeq record:
NM_001242560.2
NBCI Gene record:
MAP4K4 (9448)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

Clone ID Target Seq Vector PAM Seq Cut Position Cut % of CDS Length Exon On Target Score[?] Other Matching Genes Orig. Target Gene[?] Notes Addgene[?]
1 BRDN0001162353 GCGGGGCTTTACCAGTAACA pXPR_003 TGG 1477 40% 14 0.4851 MAP4K4 MAP4K4 76266
2 BRDN0001162594 ACTCACACCAAAGTCAACTG pXPR_003 TGG 508 14% 7 0.4474 MAP4K4 MAP4K4 76264
3 BRDN0001148610 ACGGCCACCTCACAAAGTAA pXPR_003 CGG 2269 61% 21 0.2697 MAP4K4 MAP4K4 76263
4 BRDN0001146036 CTCACCAGGCACGTTCACAA pXPR_003 TGG 1030 28% 12 0.0385 MAP4K4 MAP4K4 76265
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shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Human NM_001242560.2, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Human Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000195258 CCTACATCAGTAGCATATATT pLKO.1 3846 CDS 100% 15.000 21.000 N MAP4K4 n/a
2 TRCN0000025206 GCCTTTAAGTCATTTGGAGAA pLKO.1 3540 CDS 100% 4.050 3.240 N Map4k4 n/a
3 TRCN0000220094 TCCTTCCTGTTCCTCTTATAT pLKO.1 4227 3UTR 100% 15.000 10.500 N MAP4K4 n/a
4 TRCN0000219682 ATTTGGAGAATTGGTACATAA pLKO.1 3551 CDS 100% 13.200 9.240 N MAP4K4 n/a
5 TRCN0000195121 CACAGAAACATTGCAACATAT pLKO.1 609 CDS 100% 13.200 9.240 N MAP4K4 n/a
6 TRCN0000219681 CACCTATGGACAAGTCTATAA pLKO.1 476 CDS 100% 13.200 9.240 N MAP4K4 n/a
7 TRCN0000025205 GCACCTATGGACAAGTCTATA pLKO.1 475 CDS 100% 13.200 9.240 N Map4k4 n/a
8 TRCN0000322213 GCACCTATGGACAAGTCTATA pLKO_005 475 CDS 100% 13.200 9.240 N Map4k4 n/a
9 TRCN0000220091 GAGAAAGATGAAACTGAGTAT pLKO.1 1317 CDS 100% 4.950 3.465 N MAP4K4 n/a
10 TRCN0000220092 GTCTACTATTTGTCCTGGTTA pLKO.1 3333 CDS 100% 4.950 3.465 N MAP4K4 n/a
11 TRCN0000199325 CAGGGATTACTGGCCTCCAGA pLKO.1 4096 3UTR 100% 0.880 0.616 N MAP4K4 n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript NM_001242560.2, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_14940 pDONR223 22.5% 93.1% 18.5% None 685delG;1611_1841del;3324_3325ins24 n/a
2 ccsbBroad304_14940 pLX_304 0% 93.1% 18.5% V5 (not translated due to prior stop codon) 685delG;1611_1841del;3324_3325ins24 n/a
3 TRCN0000469592 CGGCACGATTGGGGAAGCGATCCT pLX_317 10.3% 93.1% 18.5% V5 (not translated due to prior stop codon) 685delG;1611_1841del;3324_3325ins24 n/a
4 TRCN0000487793 TGGAGTGGCTTACGTAAATCTGCC pLX_317 7.3% 86.8% 86.8% V5 (not translated due to prior stop codon) (many diffs) n/a
5 TRCN0000489356 CACTGAGATTGGACCATACTAGGT pLX_317 8.1% 86.7% 86.7% V5 (not translated due to prior stop codon) (many diffs) n/a
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