Construct: ORF TRCN0000487850
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF021734.1_s317c1
- DNA Barcode:
- TTTCTCTGAGTTGCTCGTATCGCG
- Epitope Tag:
- V5 (not translated due to prior stop codon)
- Notes:
- Has stop codon in insert
Originally Annotated References:
- Gene:
- FGFR1 (2260)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000487850
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 2260 | FGFR1 | fibroblast growth factor re... | XM_017013230.1 | 82.1% | 82.1% | 1_267del |
| 2 | human | 2260 | FGFR1 | fibroblast growth factor re... | XM_017013229.2 | 74.1% | 68.8% | (many diffs) |
| 3 | human | 2260 | FGFR1 | fibroblast growth factor re... | NM_001354368.2 | 56.1% | 56.1% | 1_960del |
| 4 | human | 2260 | FGFR1 | fibroblast growth factor re... | NM_023106.3 | 55.9% | 55.9% | 1_960del;1006_1011delGTAACA |
| 5 | human | 2260 | FGFR1 | fibroblast growth factor re... | NM_001174066.2 | 55.7% | 55.7% | 1_966del;1012_1017delGTAACA |
| 6 | human | 2260 | FGFR1 | fibroblast growth factor re... | NM_023105.3 | 55.7% | 55.7% | 1_966del;1012_1017delGTAACA |
| 7 | human | 2260 | FGFR1 | fibroblast growth factor re... | XM_017013227.1 | 53.6% | 53.6% | 1_1059del |
| 8 | human | 2260 | FGFR1 | fibroblast growth factor re... | XM_011544450.2 | 53.5% | 53.5% | 1_1059del;1105_1110delGTAACA |
| 9 | human | 2260 | FGFR1 | fibroblast growth factor re... | XM_017013226.1 | 53.5% | 53.5% | 1_1065del |
| 10 | human | 2260 | FGFR1 | fibroblast growth factor re... | XM_011544451.1 | 51.6% | 48.1% | (many diffs) |
| 11 | human | 2260 | FGFR1 | fibroblast growth factor re... | NM_001354370.1 | 51.1% | 47.7% | (many diffs) |
| 12 | human | 2260 | FGFR1 | fibroblast growth factor re... | XM_006716314.2 | 51.1% | 47.7% | (many diffs) |
| 13 | human | 2260 | FGFR1 | fibroblast growth factor re... | XM_006716310.3 | 51% | 47.6% | (many diffs) |
| 14 | human | 2260 | FGFR1 | fibroblast growth factor re... | XM_006716311.1 | 51% | 47.6% | (many diffs) |
| 15 | human | 2260 | FGFR1 | fibroblast growth factor re... | XM_006716312.2 | 51% | 47.6% | (many diffs) |
| 16 | human | 2260 | FGFR1 | fibroblast growth factor re... | NM_001174064.2 | 50.3% | 50.3% | 1_1209del |
| 17 | human | 2260 | FGFR1 | fibroblast growth factor re... | XM_017013224.2 | 50% | 50% | 1_1227del |
| 18 | human | 2260 | FGFR1 | fibroblast growth factor re... | XM_017013225.2 | 50% | 50% | 1_1227del |
| 19 | human | 2260 | FGFR1 | fibroblast growth factor re... | NM_001174063.2 | 49.8% | 49.8% | 1_1233del |
| 20 | human | 2260 | FGFR1 | fibroblast growth factor re... | NM_001174065.2 | 49.8% | 49.8% | 1_1227del;1273_1278delGTAACA |
| 21 | human | 2260 | FGFR1 | fibroblast growth factor re... | NM_015850.4 | 49.8% | 49.8% | 1_1227del;1273_1278delGTAACA |
| 22 | human | 2260 | FGFR1 | fibroblast growth factor re... | NM_023110.3 | 49.7% | 49.7% | 1_1233del;1279_1284delGTAACA |
| 23 | human | 2260 | FGFR1 | fibroblast growth factor re... | XM_011544449.1 | 49% | 45.7% | (many diffs) |
| 24 | human | 2260 | FGFR1 | fibroblast growth factor re... | XM_011544448.1 | 48.9% | 45.6% | (many diffs) |
| 25 | human | 2260 | FGFR1 | fibroblast growth factor re... | XM_017013220.1 | 48% | 48% | 1_1326del |
| 26 | human | 2260 | FGFR1 | fibroblast growth factor re... | NM_001174067.1 | 47.9% | 47.9% | 1_1326del;1372_1377delGTAACA |
| 27 | human | 2260 | FGFR1 | fibroblast growth factor re... | XM_011544447.2 | 47.9% | 47.9% | 1_1332del |
| 28 | human | 2260 | FGFR1 | fibroblast growth factor re... | XM_011544446.2 | 47.8% | 47.8% | 1_1332del;1378_1383delGTAACA |
| 29 | human | 2260 | FGFR1 | fibroblast growth factor re... | XM_024447097.1 | 46.1% | 43% | (many diffs) |
| 30 | human | 2260 | FGFR1 | fibroblast growth factor re... | NM_001354369.1 | 45.8% | 42.8% | (many diffs) |
| 31 | human | 2260 | FGFR1 | fibroblast growth factor re... | NM_001354367.1 | 45.7% | 42.7% | (many diffs) |
| 32 | human | 2260 | FGFR1 | fibroblast growth factor re... | XM_006716307.1 | 45.7% | 42.7% | (many diffs) |
| 33 | human | 2260 | FGFR1 | fibroblast growth factor re... | XM_017013222.2 | 45.7% | 42.7% | (many diffs) |
| 34 | human | 2260 | FGFR1 | fibroblast growth factor re... | XM_006716303.3 | 45.6% | 42.6% | (many diffs) |
| 35 | human | 2260 | FGFR1 | fibroblast growth factor re... | XM_006716304.1 | 45.6% | 42.6% | (many diffs) |
| 36 | human | 2260 | FGFR1 | fibroblast growth factor re... | XM_017013221.1 | 45.6% | 42.6% | (many diffs) |
| 37 | human | 2260 | FGFR1 | fibroblast growth factor re... | XM_017013219.1 | 44.1% | 41.2% | (many diffs) |
| 38 | human | 2260 | FGFR1 | fibroblast growth factor re... | XM_011544444.1 | 44% | 41.1% | (many diffs) |
| 39 | human | 2260 | FGFR1 | fibroblast growth factor re... | XM_011544445.2 | 44% | 41.1% | (many diffs) |
| 40 | human | 2260 | FGFR1 | fibroblast growth factor re... | XM_011544443.2 | 43.9% | 41% | (many diffs) |
| 41 | human | 2260 | FGFR1 | fibroblast growth factor re... | XR_001745495.1 | 33.6% | (many diffs) | |
| 42 | human | 2260 | FGFR1 | fibroblast growth factor re... | XR_001745496.1 | 28.5% | (many diffs) | |
| 43 | mouse | 14182 | Fgfr1 | fibroblast growth factor re... | XM_011242121.1 | 50.2% | 55.5% | (many diffs) |
| 44 | mouse | 14182 | Fgfr1 | fibroblast growth factor re... | NM_001079909.2 | 50.1% | 55.3% | (many diffs) |
| 45 | mouse | 14182 | Fgfr1 | fibroblast growth factor re... | NM_001079908.2 | 44.8% | 49.5% | (many diffs) |
| 46 | mouse | 14182 | Fgfr1 | fibroblast growth factor re... | XM_006509013.1 | 44.8% | 49.5% | (many diffs) |
| 47 | mouse | 14182 | Fgfr1 | fibroblast growth factor re... | XM_011242120.1 | 44.8% | 49.5% | (many diffs) |
| 48 | mouse | 14182 | Fgfr1 | fibroblast growth factor re... | NM_010206.3 | 44.7% | 49.3% | (many diffs) |
| 49 | mouse | 14182 | Fgfr1 | fibroblast growth factor re... | XM_006509010.2 | 44.7% | 49.3% | (many diffs) |
| 50 | mouse | 14182 | Fgfr1 | fibroblast growth factor re... | XM_006509012.1 | 44.7% | 49.3% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 78
- ORF end:
- 1305
- ORF length:
- 1227
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 caggcttcca cagccagatg gctgtgcaca agctggccaa gagcatccct ctgcgcagac 121 aggtgtctgc tgactccagt gcatccatga actctggggt tcttctggtt cggccatcac 181 ggctctcctc cagtgggact cccatgctag caggggtctc tgagtatgag cttcccgaag 241 accctcgctg ggagctgcct cgggacagac tggtcttagg caaacccctg ggagagggct 301 gctttgggca ggtggtgttg gcagaggcta tcgggctgga caaggacaaa cccaaccgtg 361 tgaccaaagt ggctgtgaag atgttgaagt cggacgcaac agagaaagac ttgtcagacc 421 tgatctcaga aatggagatg atgaagatga tcgggaagca taagaatatc atcaacctgc 481 tgggggcctg cacgcaggat ggtcccttgt atgtcatcgt ggagtatgcc tccaagggca 541 acctgcggga gtacctgcag gcccggaggc ccccagggct ggaatactgc tacaacccca 601 gccacaaccc agaggagcag ctctcctcca aggacctggt gtcctgcgcc taccaggtgg 661 cccgaggcat ggagtatctg gcctccaaga agtgcataca ccgagacctg gcagccagga 721 atgtcctggt gacagaggac aatgtgatga agatagcaga ctttggcctc gcacgggaca 781 ttcaccacat cgactactat aaaaagacaa ccaacggccg actgcctgtg aagtggatgg 841 cacccgaggc attatttgac cggatctaca cccaccagag tgatgtgtgg tctttcgggg 901 tgctcctgtg ggagatcttc actctgggcg gctccccata ccccggtgtg cctgtggagg 961 aacttttcaa gctgctgaag gagggtcacc gcatggacaa gcccagtaac tgcaccaacg 1021 agctgtacat gatgatgcgg gactgctggc atgcagtgcc cTCACAGAGA CCCACCTTCA 1081 AGCAGCTGGT GGAAGACCTG GACCGCATCG TGGCCTTGAC CTCCAACCAG GAGTACCTGG 1141 ACCTGTCCAT GCCCCTGGAC CAGTACTCCC CCAGCTTTCC CGACACCCGG AGCTCTACGT 1201 GCTCCTCAGG GGAGGATTCC GTCTTCTCTC ATGAGCCGCT GCCCGAGGAG CCCTGCCTGC 1261 CCCGACACCC AGCCCAGCTT GCCAATGGCG GACTCAAACG CCGCTGAGAC CCAGCTTTCT 1321 TGTACAAAGT GGTTGATATC GGTAAGCCTA TCCCTAACCC TCTCCTCGGT CTCGATTCTA 1381 CGTAGTAATG AACTAGTCCG TAACTTGAAA GTATTTCGAT TTCTTGGCTT TATATATCTT 1441 GTGGAAAGGA CGATTTCTCT GAGTTGCTCG TATCGCGACG CGTTAAGTCg acaatcaacc 1501 tctggattac aaaatttgtg aaagatt