Construct: ORF TRCN0000488171
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF021443.1_s317c1
- DNA Barcode:
- TCATCTCCCGTACCTCATGACTTG
- Epitope Tag:
- V5 (not translated due to prior stop codon)
- Notes:
- Has stop codon in insert
Originally Annotated References:
- Gene:
- ENTPD1 (953)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000488171
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 953 | ENTPD1 | ectonucleoside triphosphate... | NM_001776.6 | 100% | 100% | |
| 2 | human | 953 | ENTPD1 | ectonucleoside triphosphate... | NM_001098175.2 | 98.5% | 97.8% | (many diffs) |
| 3 | human | 953 | ENTPD1 | ectonucleoside triphosphate... | XM_011540371.2 | 98.5% | 97.8% | (many diffs) |
| 4 | human | 953 | ENTPD1 | ectonucleoside triphosphate... | NM_001164178.1 | 97.3% | 96.7% | (many diffs) |
| 5 | human | 953 | ENTPD1 | ectonucleoside triphosphate... | NM_001164179.2 | 91.9% | 91.9% | 810_811ins123 |
| 6 | human | 953 | ENTPD1 | ectonucleoside triphosphate... | XM_011540370.2 | 91.2% | 85.2% | (many diffs) |
| 7 | human | 953 | ENTPD1 | ectonucleoside triphosphate... | XM_017016958.2 | 89.5% | 85.9% | (many diffs) |
| 8 | human | 953 | ENTPD1 | ectonucleoside triphosphate... | NM_001320916.1 | 87% | 83% | (many diffs) |
| 9 | human | 953 | ENTPD1 | ectonucleoside triphosphate... | XM_011540372.2 | 81.3% | 72.4% | (many diffs) |
| 10 | human | 953 | ENTPD1 | ectonucleoside triphosphate... | XM_011540373.2 | 80.1% | 72.3% | (many diffs) |
| 11 | human | 953 | ENTPD1 | ectonucleoside triphosphate... | XM_011540374.3 | 79.6% | 73.1% | (many diffs) |
| 12 | human | 953 | ENTPD1 | ectonucleoside triphosphate... | NM_001164181.1 | 78.8% | 78.8% | 0_1ins324 |
| 13 | human | 953 | ENTPD1 | ectonucleoside triphosphate... | NM_001312654.1 | 78.8% | 78.8% | 0_1ins324 |
| 14 | human | 953 | ENTPD1 | ectonucleoside triphosphate... | XM_011540377.2 | 78.8% | 78.8% | 0_1ins324 |
| 15 | human | 953 | ENTPD1 | ectonucleoside triphosphate... | NM_001164182.2 | 72.9% | 72.9% | 0_1ins414 |
| 16 | human | 953 | ENTPD1 | ectonucleoside triphosphate... | NM_001164183.2 | 72.9% | 72.9% | 0_1ins414 |
| 17 | human | 953 | ENTPD1 | ectonucleoside triphosphate... | XM_017016959.1 | 72.9% | 72.9% | 0_1ins414 |
| 18 | human | 953 | ENTPD1 | ectonucleoside triphosphate... | XM_017016960.1 | 68.5% | 65% | (many diffs) |
| 19 | human | 953 | ENTPD1 | ectonucleoside triphosphate... | XM_011540376.2 | 67.3% | 56.6% | (many diffs) |
| 20 | human | 953 | ENTPD1 | ectonucleoside triphosphate... | XM_017016961.1 | 62.6% | 59.2% | (many diffs) |
| 21 | human | 953 | ENTPD1 | ectonucleoside triphosphate... | XM_017016964.2 | 53.9% | 53.3% | (many diffs) |
| 22 | human | 953 | ENTPD1 | ectonucleoside triphosphate... | XM_017016963.1 | 52.3% | 51.1% | (many diffs) |
| 23 | human | 953 | ENTPD1 | ectonucleoside triphosphate... | XM_017016962.1 | 47.6% | 42.1% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 63
- ORF end:
- 1593
- ORF length:
- 1530
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaagcca 61 ccatggaaga tacaaaggag tctaacgtga agacattttg ctccaagaat atcctagcca 121 tccttggctt ctcctctatc atagctgtga tagctttgct tgctgtgggg ttgacccaga 181 acaaagcatt gccagaaaac gttaagtatg ggattgtgct ggatgcgggt tcttctcaca 241 caagtttata catctataag tggccagcag aaaaggagaa tgacacaggc gtggtgcatc 301 aagtagaaga atgcagggtt aaaggtcctg gaatctcaaa atttgttcag aaagtaaatg 361 aaataggcat ttacctgact gattgcatgg aaagagctag ggaagtgatt ccaaggtccc 421 agcaccaaga gacacccgtt tacctgggag ccacggcagg catgcggttg ctcaggatgg 481 aaagtgaaga gttggcagac agggttctgg atgtggtgga gaggagcctc agcaactacc 541 cctttgactt ccagggtgcc aggatcatta ctggccaaga ggaaggtgcc tatggctgga 601 ttactatcaa ctatctgctg ggcaaattca gtcagaaaac aaggtggttc agcatagtcc 661 catatgaaac caataatcag gaaacctttg gagctttgga ccttggggga gcctctacac 721 aagtcacttt tgtaccccaa aaccagacta tcgagtcccc agataatgct ctgcaatttc 781 gcctctatgg caaggactac aatgtctaca cacatagctt cttgtgctat gggaaggatc 841 aggcactctg gcagaaactg gccaaggaca ttcaggttgc aagtaatgaa attctcaggg 901 acccatgctt tcatcctgga tataagaagg tagtgaacgt aagtgacctt tacaagaccc 961 cctgcaccaa gagatttgag atgactcttc cattccagca gtttgaaatc cagggtattg 1021 gaaactatca acaatgccat caaagcatcc tggagctctt caacaccagt tactgccctt 1081 actcccagtg tgccttcaat gggattttct tgccaccact ccagggggat tttggggcat 1141 tttcagcttt ttactttgtg atgaagtttt taaacttgac atcagagaaa gtctctcagg 1201 aaaaggtgac tgagatgatg aaaaagttct gtgctcagcc ttgggaggag ataaaaacat 1261 cttacgctgg agtaaaggag aagtaccTGA GTGAATACTG CTTTTCTGGT ACCTACATTC 1321 TCTCCCTCCT TCTGCAAGGC TATCATTTCA CAGCTGATTC CTGGGAGCAC ATCCATTTCA 1381 TTGGCAAGAT CCAGGGCAGC GACGCCGGCT GGACTTTGGG CTACATGCTG AACCTGACCA 1441 ACATGATCCC AGCTGAGCAA CCATTGTCCA CACCTCTCTC CCACTCCACC TATGTCTTCC 1501 TCATGGTTCT ATTCTCCCTG GTCCTTTTCA CAGTGGCCAT CATAGGCTTG CTTATCTTTC 1561 ACAAGCCTTC ATATTTCTGG AAAGATATGG TATAGGACCC AGCTTTCTTG TACAAAGTGG 1621 TTGATATCGG TAAGCCTATC CCTAACCCTC TCCTCGGTCT CGATTCTACG TAGTAATGAA 1681 CTAGTCCGTA ACTTGAAAGT ATTTCGATTT CTTGGCTTTA TATATCTTGT GGAAAGGACG 1741 ATCATCTCCC GTACCTCATG ACTTGACGCG TTAAGTCgac aatcaacctc tggattacaa 1801 aatttgtgaa agatt